Preparation of rumen bypass coated bacteriophage and application of rumen bypass coated bacteriophage in prevention and treatment of buffalo diarrhea
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A phage and rumen-passing technology, applied in the field of microbiology and animal husbandry
Inactive Publication Date: 2021-06-22
GUANGXI UNIV
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However, how to select and screen suitable phages for assembly is a difficult point res
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Embodiment 1
[0027] Example 1: Screening for phages with higher correlation
[0028] Based on the sequencing of virus particles and DNA in buffalo rectal feces, combined with the bioinformatics analysis of buffalo, the phages with high correlation were screened.
[0029] In this example, 20 buffaloes in the breeding base of Guangxi Huaxu Buffalo Biotechnology Co., Ltd. were selected, among which 10 were healthy and 10 were suffering from diarrheal diseases. Feces samples were taken directly from the rectum of buffaloes, and each sample was divided into two parts. The sample is used to extract virions, which are sequenced by third-generation PacBio to obtain the genome sequences of bacteriophages and other viruses; another sample is used to extract DNA for direct third-generation PacBio sequencing, and the sequencing results are assembled and followed by bioinformatics analysis; GO (commonly used database for gene function analysis) and KEGG (commonly used database and tools for metabolic p...
Embodiment 2
[0030] Embodiment 2: breeding test verification
[0031] Firstly, rumen-coated phages were prepared, which were assembled from six kinds of phages, including: Staphylococcus aureus phage 52, Staphylococcus aureus subspecies phage B1, coliphage T1, coliphage T3, Escherichia coli phage S13, and Bacillus subtilis phage phi-e; the above six phages were purchased from ATCC in the United States;
[0032] The preparation method of described rumen-coated phage comprises the steps:
[0033]S1. Mix the freeze-dried powders of the above six bacteriophages and auxiliary materials evenly in a weight ratio of 3:10;
[0034] S2. Adding a 2% aqueous solution of hydroxypropyl methylcellulose to the above mixture to prepare a soft material;
[0035] S3, making the above-mentioned soft materials into strips, and cutting, rounding, and drying to obtain pellet cores;
[0036] S4, above-mentioned ball core is added in the air fluidized bed coating machine, the coating solution that sprays in, pr...
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Abstract
The invention relates to preparation of rumen bypass coated bacteriophage and an application of the rumen bypass coated bacteriophage in prevention and treatment of buffalo diarrhea. The rumen bypass coated bacteriophage is prepared by compounding six bacteriophages, comprising a staphylococcus aureus bacteriophage 52, a staphylococcus aureus subspecies bacteriophage B1, an escherichia coli bacteriophage T1, an escherichia coli bacteriophage T3, an escherichia coli bacteriophage S13 and a bacillus subtilis bacteriophage phi-e. The rumen bypass coated bacteriophage provided by the invention is added into a buffalo concentrate supplement according to the proportion that 30-70kg of the rumen bypass coated bacteriophage is added into each ton of concentrate. The rumen bypass coated bacteriophage is administrated to buffalo in a rumen bypass coating mode, the effect of preventing and treating buffalo diarrhea is ideal, and reference is provided for research of screening specific bacteriophages in the later period.
Description
technical field [0001] The invention relates to the field of microorganisms and animal husbandry, in particular to the preparation of a rumen-passing coated phage and its application in the prevention and treatment of buffalo diarrhea. [0002] technical background [0003] Buffalo has biological characteristics such as strong adaptability, high temperature and high humidity resistance, strong disease resistance, rough feeding resistance, easy breeding, and long service life. The development of the buffalo industry is of great significance to targeted poverty alleviation and improving farmers' economic income in Guangxi and other provinces . Diarrhea is a disease with high morbidity, difficulty in treatment and high mortality in buffaloes. It can be divided into three types: septicemia, enterotype and enterotoxic blood type. Diseases caused by intestinal bacteria are one of the main pathogenic factors of buffaloes. The widespread use of antibiotics can control intestinal bac...
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