Graphene-based polymer composite nerve conduit and preparation method thereof

Abstract

The invention provides a preparation method of a graphene-based polymer composite nerve conduit. The preparation method comprises the following steps: (1) preparing graphene oxide: at a first stage, preparing expanded graphite; at a second stage, preparing high-concentration graphene oxide dispersion liquid; (2) preparing printing ink, wherein a sodium alginate solution is printing ink I; an ethylenediamine solution is added into the graphene dispersion liquid to obtain printing ink II; (3) carrying out 3D printing of the rGO catheter: adopting a coaxial bi-material 3D printing needle, enabling the ink I to flow out through a main printing needle of a 3D printer and the ink II to flow out through an auxiliary printing needle, and forming the macroscopic three-dimensional rGO catheter and freeze-drying for standby application; and (4) preparing a graphene-based polymer tube: burning and reducing the rGO conduit, dissolving the polymer in dichloromethane, soaking the rGO conduit in the liquid, fishing out the rGO conduit after soaking for a period of time, and air-drying under natural conditions to complete preparation of the graphene-based polymer composite nerve conduit.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a graphene-based polymer composite nerve guide and a preparation method thereof. Background technique [0002] Peripheral nerves play an important role in supporting structural stability in the motor and sensory nervous system. Its destruction can lead to paralysis of patients and loss of essential functions. The number of peripheral nerve injuries is increasing every year, posing a great threat to human health. . At present, autologous nerve transplantation is the gold standard for the treatment of severe peripheral nerve defects. However, autologous nerve transplantation has great limitations, such as a serious shortage of donors and inevitable secondary damage to the donor site. Therefore, the artificial nerve repair catheter has become a hot spot in modern medical research. [0003] A good neural implant should have the following characteristics: 1. Good neurocom...

AI Technical Summary

Problems solved by technology

However, it is difficult for a single material to meet the above characteristics, so people have developed a variety of composite materials, among which the composite of polymer and graphene materials is particularly important

[0004] Graphene materials have been proven to have good biocompatibility and electrical conductivity, but at present, most of its applications in nerve conduits are based on other materials. Graphene is mixed into printing ink as an additive material, or coated as a coating on On the surface of nerve guides made of other materials, the carrying of the conductive network depends on the random stacking of graphene sheets. The porous structure mainly comes from the preparation process (electrospinning or printing molds, etc.), which greatly reduces the graphene The applicability and processability of materials in nerve tissue engineering materials, therefore, it is particularly necessary to develop a nerve guide with graphene three-dimensional network tubular structure as the main body and other polymer materials as auxiliary materials

Images