Tea saponin purification method

A purification method and technology of tea saponin, applied in the direction of chemical instruments and methods, organic chemistry, steroids, etc., can solve the problems of high solubility in supercritical extraction, reduce the contact range of materials, and reduce the yield of tea saponin, etc., to achieve The effect of expanding the target range, improving the infiltration effect, and improving the extraction rate

Active Publication Date: 2021-06-25
贵州乔盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is environmentally friendly and easy to operate. However, the equipment is complicated and not suitable for most people. In addition, the supercritical extraction itself has a high solubility, and the accuracy in a small range is very poor during extraction, and it is easy to trap many structural analogues. ; Another example is that the patent number is CN201911294499.0, a rapid refining method of tea saponin, which first uses hydrous ethanol to quickly extract tea saponin at a lower temperature, and concentrates to remove the organic solvent, and then uses a small molecule organic solvent-salt double water The phase extraction system separates most of the polar impurities at one time, and finally removes salt and organic solvents, and drying can obtain high-purity tea saponin products. Since it is treated with solvents at the beginning, the contact range of materials is reduced, making tea soap Therefore, it is extremely necessary to develop a method for purifying tea saponin that is easy to operate and is conducive to improving the purity of tea saponin.

Method used

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Experimental program
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Embodiment 1

[0022] A method for purifying tea saponin, comprising the following steps:

[0023] (1) Camellia oleifera seed meal treatment

[0024] Crush the camellia seed meal into 100-mesh powder, dry at 0.1 standard atmospheric pressure and 15°C for 3 hours, mix the powder with pure water 10 times its mass, adjust the pH of the mixed solution to 5.3 with acetic acid solution, and add 5% of the mass of the mixed solution Stir at 80r / min for 50min at 40°C, adjust the pH of the mixture to 8 with calcium hydroxide solution, add 2% protease to the mixture, and stir at 100r / min for 30min at 53°C , the mixed solution is spray-dried, and dried at 0.01 standard atmospheric pressure and 15° C. for 30 minutes to obtain enzymatic hydrolysis camellia seed meal; the mass fraction of the acetic acid solution is 8%; the mass fraction of the calcium hydroxide solution is 0.3%;

[0025] (2) Crude extraction of tea saponin

[0026] Mix the enzymolyzed camellia oleifera seed meal with the extraction solv...

Embodiment 2

[0030] A method for purifying tea saponin, comprising the following steps:

[0031] (1) Camellia oleifera seed meal treatment

[0032] Crush the camellia seed meal into 200-mesh powder, dry at 0.3 standard atmospheric pressure and 20°C for 5 hours, mix the powder with pure water 13 times its mass, adjust the pH of the mixture to 5.5 with acetic acid solution, and add 8% of the mass of the mixture Stir at 200r / min for 80min at 45°C, then adjust the pH of the mixture to 8.5 with calcium hydroxide solution, add 3% protease in the mass of the mixture, and stir at 150r / min for 50min at 55°C , the mixed solution is spray-dried, and dried at 0.03 standard atmospheric pressure and 20° C. for 50 minutes to obtain enzymatic hydrolysis camellia oleifera seed meal; the mass fraction of the acetic acid solution is 12%; the mass fraction of the calcium hydroxide solution is 0.5%;

[0033] (2) Crude extraction of tea saponin

[0034] Mix the enzymolyzed camellia oleifera seed meal with the...

Embodiment 3

[0038] A method for purifying tea saponin, comprising the following steps:

[0039] (1) Camellia oleifera seed meal treatment

[0040]Crush camellia seed meal into 200-mesh powder, dry at 0.1 standard atmospheric pressure and 18°C ​​for 4 hours, mix the powder with pure water 13 times its mass, adjust the pH of the mixed solution to 5.3 with acetic acid solution, and add 8% of the mixed solution Cellulase, stirred at 200r / min at 40°C for 50min, then adjusted the pH of the mixed solution to 8.5 with calcium hydroxide solution, added protease with 2% mass of the mixed solution, and stirred at 100r / min at 55°C for 50min, The mixed solution was spray-dried, and dried at 0.03 standard atmospheric pressure and 15° C. for 35 minutes to obtain enzymolyzed Camellia oleifera seed meal; the mass fraction of the acetic acid solution was 11%; the mass fraction of the calcium hydroxide solution was 0.45%;

[0041] (2) Crude extraction of tea saponin

[0042] Mix the enzymolyzed Camellia o...

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PUM

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Abstract

The invention relates to the technical field of tea saponin, in particular to a tea saponin purification method. The method comprises the following steps: (1) camellia seed meal treatment; (2) tea saponin crude extraction; and (3) tea saponin crude product refining. According to the invention, the camellia seed meal is crushed and dried, and impurities in the camellia seed meal are further removed, so that material gaps are large, and more space is provided for contacting with a solvent; the camellia seed meal is decomposed by mixing water and adding enzyme, so that the cell molecular structure of the camellia seed meal is dispersed, and the infiltration effect of the solvent is improved; and then spray drying is performed, so that moisture is volatilized, and complete components of the materials are kept. By using the extraction solvent formed by mixing the deionized water, the absolute ethyl alcohol and the ethyl acetate, the dissolvable target range is expanded, the tea saponin combined with other water-soluble and alcohol-soluble substances can be extracted more easily, the extraction rate of the tea saponin can be remarkably improved, and the prepared tea saponin is higher in activity and more in application.

Description

technical field [0001] The invention relates to the technical field of tea saponin, in particular to a method for purifying tea saponin. Background technique [0002] Camellia oleifera is the basic edible oil species in my country, and my country is also the country with the largest area of ​​cultivated camellia oleifera in the world. Camellia oleifera seed meal contains 10%-16% tea saponin, which is an important source of tea saponin. In addition, it also contains rich polysaccharides and proteins, and is an excellent feed. [0003] Along with the industrialized extraction of tea saponin and the development of application research, the production technology of tea saponin is constantly improving, but there is no big breakthrough so far. Conventional extraction processes mainly include extraction processes such as water extraction, hydrous ethanol, and hydrous methanol. In particular, auxiliary measures such as supercritical extraction and microbial decomposition are also u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07J63/008C07H15/256C07H1/08
Inventor 张梦福刘见祥郭光平李梦萱
Owner 贵州乔盛生物科技有限公司
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