Long-acting recombinant elastin expressed by saccharomyces cerevisiae and application of the long-acting recombinant elastin in cosmetics

A technology of elastin and Saccharomyces cerevisiae, applied in the emerging biomedical field, can solve problems such as heterologous difficulties, and achieve the effect of low rejection reaction, no virus and low hidden danger

Pending Publication Date: 2021-07-02
WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the existing technical problems: due to the macromolecular nature of natural elastin, it is difficult to directly heterologous to its original sequence, the present invention provides a long-acting recombinant elastin expressed by Saccharomyces cerevisiae and its application in cosmetics

Method used

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  • Long-acting recombinant elastin expressed by saccharomyces cerevisiae and application of the long-acting recombinant elastin in cosmetics
  • Long-acting recombinant elastin expressed by saccharomyces cerevisiae and application of the long-acting recombinant elastin in cosmetics
  • Long-acting recombinant elastin expressed by saccharomyces cerevisiae and application of the long-acting recombinant elastin in cosmetics

Examples

Experimental program
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Effect test

Embodiment 1

[0024] This embodiment provides a long-acting recombinant elastin expressed by Saccharomyces cerevisiae, and the long-acting recombinant elastin has good biocompatibility, certain antioxidant activity and reducing ability. Moreover, the advantages of the long-acting recombinant elastin compared with the elastin extracted from animal tissues and variants are its solubility, no hidden danger of virus and low rejection reaction.

[0025] The nucleotide sequence of the long-acting recombinant elastin is shown in Seq ID NO.1. The amino acid sequence corresponding to the nucleotide sequence of the long-acting recombinant elastin is shown in Seq ID NO.2.

[0026] The nucleotide sequence of long-acting recombinant elastin is obtained from the original sequence of natural elastin after yeast codon optimization, mainly through the artificial design of the key hexapeptide repeat sequence GVGVAP in natural elastin.

[0027] The nucleotide sequence obtained after the original sequence of ...

Embodiment 2

[0036] In order to verify the antioxidant activity of the long-acting recombinant elastin expressed by Saccharomyces cerevisiae described in Example 1 on superoxide anion, this example provides the detection of the scavenging activity of the long-acting recombinant elastin in Example 1 on superoxide anion .

[0037] The detection reagents used in this detection include: superoxide anion scavenging activity detection kit purchased from Solarbio, product number BC1410, specification 50 tubes / 48 samples. The principle of the detection kit is that the AP-TEMED system generates superoxide anion, which reacts with hydroxylamine hydrochloride to generate NO 2 － , NO 2 － with sulfanilic acid and alpha － The action of naphthylamine produces a red azo compound with a characteristic absorption peak at 530nm. The scavenging ability of the sample to superoxide anion is negatively correlated with the absorbance value at 530nm.

[0038] The test includes the following measurement opera...

Embodiment 3

[0047] This embodiment provides an application of Saccharomyces cerevisiae expressing long-acting recombinant elastin in cosmetics, which uses Saccharomyces cerevisiae as described in Example 1 to express long-acting recombinant elastin. The long-acting recombinant elastin has good biocompatibility and good cell adhesion, can promote the formation of new cells, and maintain skin elasticity. And as described in Example 2, the long-acting recombinant elastin in Example 1 also has superoxide radical scavenging activity. It can be seen that the long-acting recombinant elastin described in Example 1 has broad application prospects in cosmetics.

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Abstract

The invention discloses a long-acting recombinant elastin expressed by saccharomyces cerevisiae and application of the long-acting recombinant elastin in cosmetics. The nucleotide sequence of the long-acting recombinant elastin is shown as Seq ID NO.1. The nucleotide sequence of the long-acting recombinant elastin is obtained by optimizing an original sequence of natural elastin through a yeast codon, and is mainly obtained by artificially designing a key hexapeptide repetitive sequence GVGVAP. The long-acting recombinant elastin provided by the invention has good biocompatibility, antioxidant activity and reducing ability. Compared with the elastin extracted from animal tissues and variants, the long-acting recombinant elastin has the advantages of solubility, no virus hidden danger and low rejection reaction. Compared with the elastin extracted from animal tissues and variants, the long-acting recombinant elastin provided by the invention has the advantages of solubility, no virus hidden danger and low rejection reaction.

Description

technical field [0001] The invention relates to the emerging biomedical field, in particular to a long-acting recombinant elastin expressed by Saccharomyces cerevisiae and an application of the long-acting recombinant elastin expressed by Saccharomyces cerevisiae in cosmetics. Background technique [0002] Native elastin is a low water-soluble, amorphous, highly hydrophobic, and extensively cross-linked extracellular matrix protein. The human tropoelastin domain is characterized by a hydrophobic domain rich in non-polar amino acids, especially glycine, lysine, proline, and alanine, such as the GVGVAP hexapeptide repeat sequence, which is present in human tropoelastin There are 5 in elastin; hydrophilic domains rich in lysines and alanines involved in cross-linking, these regions usually consist of lysines separated by two to three alanines, such as AAAKAAKAA. Elastin is abundant in tissues such as the skin, lungs, ligaments and blood vessels, and plays an important role in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12N15/12C12N15/81A61K8/64A61Q19/08C12R1/865
CPCC07K14/78C12N15/81A61K8/64A61Q19/08C12N2800/22
Inventor 赵俊王梦刘洁李诚茹夏洁吴博蒋敏之
Owner WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD
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