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Photodynamic nano platform with mitochondrial targeting characteristic and preparation method and application thereof

A mitochondrial and photodynamic technology, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, nanotechnology, etc., can solve the problem of single role of nano-drug carriers, difficulty in achieving tumor treatment effects, and new applications to be developed Explore and other issues to achieve effects that are beneficial to drug loading and transportation, superior in vivo circulation and tumor uptake capabilities, and efficient cell uptake characteristics

Pending Publication Date: 2021-07-13
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the single role of flexible organosilica nanoparticles as a nano-drug carrier, it is difficult to achieve the effect of treating tumors. Combining the characteristics of mitochondrial targeting and photodynamic therapy, flexible mesoporous organosilica nanoparticles can be used in biomedical applications. has been extensively studied, but more new applications remain to be explored

Method used

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  • Photodynamic nano platform with mitochondrial targeting characteristic and preparation method and application thereof
  • Photodynamic nano platform with mitochondrial targeting characteristic and preparation method and application thereof
  • Photodynamic nano platform with mitochondrial targeting characteristic and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Synthesis of mesoporous organosilica nanoparticles (MONs):

[0037] All synthesis experiments were completed in a water bath; first, 75 ml of deionized water, 30 ml of ethanol, 1 ml of concentrated ammonia water and 0.16 g of cetyltrimethylammonium bromide were added to the reaction flask in sequence, at 35 ° C , stirred at 1100rpm for 1h, then added 0.25ml TEOS and 0.1ml TETS mixed solution into the above reaction flask, and kept stirring for 24 hours to obtain a white suspension, which was separated and washed with ethanol for 3 times. The obtained nanoparticles were dispersed in a mixed solution of 120 ml ethanol and 240 μl concentrated hydrochloric acid, and heated at 60° C. for 12 hours to remove the surfactant, and finally obtained mesoporous organic silica nanoparticles.

Embodiment 2

[0039] Surface modification: It is necessary to modify the amino group on the surface of the particle, and then react with the carboxyl group on CTPP and Ce6.

[0040]The first step is to break the disulfide bond: wash 10 mg of nanomaterials with deionized water, disperse them in a mixed solution of 1.2 ml of deionized water and 4.4 ml of dioxane, then add 0.19 g of triphenylphosphine and 80 μl of concentrated hydrochloric acid, Ultrasonic mix for 10 minutes, put into a water bath, react at 40°C and 800rpm in a nitrogen environment for two hours, wash once with alcohol, and wash twice with water.

[0041] In the second step, the treated samples were respectively dispersed in 1.2ml of water, 0.12ml of DMF and 4mg of NH2-MAL, reacted for 12 hours, washed three times with deionized water, and then dispersed in 1ml of water.

[0042] In the third step, CTPP is activated and connected to the carboxyl group of ce6. First, 1ml CTPP (20mg / ml), 1ml ce6 (20mg / ml) were mixed with 0.5ml ...

Embodiment 3

[0044] Preparation of flexible MONs-CTPP-Ce6

[0045] Centrifuge 2mg of MONs-CTPP-Ce6 with surface modification at 10000rpm, disperse in 1ml of water, add 100μL of 1M NaOH solution, place on a shaker for 15min, wash with water 3 times, and obtain flexible mesoporous organic nanoparticles (SMONs-CTPP-Ce6) , dispersed in ethanol.

[0046] Figure 4 a~c, the prepared SMONs-CTPP-Ce6 nanoparticles were observed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The flexible nanoparticles have good dispersion, uniform size, particle diameter of about 200nm, and obvious shrinkage of the surface was observed, and the thickness of the silicon layer was about 25nm.

[0047] Figure 5 d-f are high-magnification transmission electron microscope bright field and element distribution images of flexible mesoporous silicone nanoparticles. The element distribution images show that phosphorus is uniformly distributed on the surface of flexible mesoporous silico...

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Abstract

The invention belongs to the technical field of photodynamic therapy, and discloses a photodynamic nano platform with a mitochondrial targeting characteristic and a preparation method and application thereof. The photodynamic nano platform with the mitochondrial targeting characteristic is composed of flexible mesoporous organic silicon oxide, mitochondrial targeting molecules and a photosensitizer, has a collapsed surface, a large internal cavity and mesoporous channels, can efficiently load drugs and has an efficient cell uptake characteristic. The preparation method comprises the following steps: etching through an alkaline solution to obtain the flexible mesoporous organic silicon oxide shell layer, and functionally modifying the mitochondrial targeting molecules and the photosensitizer Ce6 on the surface of the flexible mesoporous organic silicon oxide shell layer. The flexible mesoporous organic silicon nanoparticles are combined with a mitochondrial targeting function, so that the intracellular phagocytic efficiency, the mitochondrial targeting capability and the photodynamic therapy effect are improved. The preparation method is simple and easy to obtain, and the obtained product is high in yield and has huge application potential in the fields of tumor photodynamic therapy and the like.

Description

technical field [0001] The invention belongs to the technical field of photodynamic therapy, and specifically relates to a photodynamic nano-platform with mitochondrial targeting properties, a preparation method and application thereof. Background technique [0002] Photodynamic therapy is an emerging tumor treatment method after surgical resection, chemotherapy and radiotherapy. Photodynamic therapy drugs, that is, photosensitizers that can selectively act on tumor sites, have the advantages of less trauma, good reproducibility, no cumulative toxicity, and fewer adverse reactions, showing more and more obvious advantages in tumor treatment. Although it has a bright prospect in tumor therapy, there are still many deficiencies in the clinical application of traditional photodynamic therapy. First of all, since photosensitizers are mostly aromatic molecules, they have poor solubility in water and are easy to aggregate, which can easily lead to skin phototoxicity. On the othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/24A61P35/00B82Y5/00B82Y40/00
CPCA61K41/0071A61K47/24A61P35/00B82Y5/00B82Y40/00
Inventor 滕兆刚史文慧
Owner NANJING UNIV OF POSTS & TELECOMM
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