Unlock instant, AI-driven research and patent intelligence for your innovation.

A preparation method and application of a pet cell carrier capable of high temperature sterilization

A high-temperature-resistant, cell-based technology, applied in biochemical equipment and methods, cell culture support/coating, physical treatment, etc., can solve the complex structure of bioreactor equipment, the inability to sterilize by radiation, and poor mechanical properties To achieve the effect of promoting cell adhesion, proliferation and expression of normal functions, maintaining mechanical properties and cytocompatibility, good mechanical properties and cytocompatibility

Active Publication Date: 2022-05-27
SUN YAT SEN UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bioreactor equipment has a complex structure, large volume, many components, including precision sensing elements and control components. Generally, it can only be sterilized by high temperature with damp heat method, and cannot be sterilized by radiation or ethylene oxide.
[0005] However, the current cell culture carriers used in bioreactors generally have problems such as poor thermal stability and poor mechanical properties, which prevent them from being sterilized together with bioreactors, which greatly increases the operational complexity of cell culture.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method and application of a pet cell carrier capable of high temperature sterilization
  • A preparation method and application of a pet cell carrier capable of high temperature sterilization
  • A preparation method and application of a pet cell carrier capable of high temperature sterilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Surface modification of PET materials to prepare cell-loaded materials that can be used for high temperature and autoclave sterilization in bioreactors (PET-thermal treatment-PDA-sterilization, PET-thermal-PDA-Gel)

[0046] Polyethylene terephthalate (PET) fiber material with high adsorption polydopamine particles attached to the surface was prepared by solution oxidation method, and then gelatin macromolecules were immobilized on the surface of PET material by genipin coupling to prepare cell carrier materials, the preparation of which includes:

[0047] (1) Heat treatment of the PET sponge material: place the PET sponge sheet material in a blue-mouth bottle, pass argon to exhaust the air in the bottle, prevent the PET material from being oxidized during the heating process, and then place it in an oil bath at 140°C Heating (DF-101S heat-collecting constant temperature heating magnetic stirrer) for 3h, and natural cooling before subsequent modification treatm...

Embodiment 2

[0052] Example 2 High-temperature and high-pressure sterilization of cell-loaded materials (PET-thermal-PDA-Gel-sterilization)

[0053] The cell-loaded material prepared in Example 1 was placed in a high-temperature autoclave (model: BBS-H1100, brand: BIOBASE) for high-temperature autoclaving (120° C., 30 min).

Embodiment 1

[0067] For Example 1 (PET-thermal-PDA-Gel), Example 2 (PET-thermal-PDA-Gel-sterilization), Comparative Example 1 (PET), Comparative Example 2 (PET-sterilization), Comparative Example 3 (PET-sterilization) -thermal), Comparative Example 4 (PET-thermal-sterilization), Comparative Example 5 (PET-thermal-PDA) and Comparative Example 6 (PET-thermal-PDA-sterilization) PET materials were observed by scanning electron microscope, and their SEM morphology Analysis as figure 1 As shown, A is the comparative example 1, which is a porous fiber structure; B is the comparative example 2, the morphology of the fiber has not changed significantly; C is the comparative example 3, it can be found that there are many nodes in the fiber, which is presumed to be inside the fiber. Grains of different sizes appear; D is the comparative example 4, the morphology of the PET fiber does not change significantly; E is the comparative example 5, the polydopamine is adhered to the surface of the fiber in t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of cell culture loading materials, and specifically relates to a preparation method and application of a high-temperature sterilization-resistant PET cell carrier. The invention first uses a heat treatment process to fully crystallize the PET, and then attaches a high-adsorption method through a solution oxidation method. A PET cell carrier was prepared after using genipin as a coupling agent to fix gelatin macromolecules. The carrier has good thermal stability, can withstand conventional high temperature and high pressure sterilization and maintains good mechanical properties and cell compatibility; at the same time, the carrier has a loose and porous structure, which can provide a three-dimensional growth space for cells and can accommodate a large number of Cell growth can be used in all kinds of commonly used culture devices, especially suitable for use as a fixed bed filler to support cell growth in large-scale bioreactors that must be packaged as a whole and sterilized at high temperature; in addition, the preparation process of the present invention is simple and can be used Simple and flexible, wide applicability.

Description

technical field [0001] The invention belongs to the technical field of cell culture load materials, and in particular relates to a preparation method and application of a high temperature sterilization-resistant PET cell carrier. Background technique [0002] Cell culture refers to the technology in which animal cells grow and proliferate in vitro and maintain their characteristics and functions under suitable culture conditions. Most cells from animal solid tissues need to grow in an adherent monolayer, and as long as they are not transformed into an anchorage-independent, they must be attached and spread on a suitable solid medium before they can begin to grow. A cell culture carrier often referred to by those skilled in the art. [0003] Traditional cell culture uses cell culture dishes, which are treated for cell attachment and growth. A traditional culture dish provides 78.52cm 2 and can support 10 6 -10 7 cell growth. Subsequently, people began to use cell spinner...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): D06M10/00D06M15/61D06M15/15C12N5/00D06M101/32
CPCD06M10/00D06M15/61D06M15/15C12N5/0062C12N2513/00C12N2533/30D06M2101/32
Inventor 龚逸鸿曹聪罗语溪李燕叶浩刘海东郑毓灵欧金淼韦秋君
Owner SUN YAT SEN UNIV