Three-dimensional porous collagen scaffold and double-layer collagen scaffold, as well as preparation methods and application thereof

Abstract

The invention provides a three-dimensional porous collagen scaffold and a double-layer collagen scaffold, as well as preparation methods and application thereof, and relates to the technical field of biomedical materials. The three-dimensional porous collagen scaffold is prepared according to the following steps: subjecting a collagen solution to freeze drying treatment, and sequentially carrying out pressing treatment and heat treatment to obtain the three-dimensional porous collagen scaffold. According to the preparation method of the three-dimensional porous collagen scaffold provided by the invention, the pressing treatment is set between the freeze drying treatment and the heat treatment, and the three-dimensional porous collagen scaffold, prepared without using the crosslinking agent, has excellent mechanical properties, degradation resistance and biocompatibility and good dimensional stability (especially in a wetting state) and thermal stability, and a three-dimensional porous structure of the collagen can still be maintained on the premise of ensuring mechanical properties, so that the three-dimensional porous collagen scaffold has an excellent tissue regeneration induction property.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method of a three-dimensional porous collagen scaffold, a three-dimensional porous collagen scaffold, a double-layer collagen scaffold, a preparation method and an application thereof. Background technique [0002] Collagen is a structural protein of the extracellular matrix, which plays a role in supporting organs and protecting the body. Collagen molecules have a unique tertiary structure. The primary structure is that amino acids such as glycine, proline and hydroxyproline are linked by peptide bonds; the secondary structure refers to the formation of a left-handed helix in space; the tertiary structure refers to three The left-handed helical polypeptide chain relies on ionic bonds, hydrogen bonds and other forces to form a right-handed superhelical structure. This superhelical structure makes the properties of collagen very stable, and has histocom...

AI Technical Summary

Problems solved by technology

Excellent biological properties make collagen materials a research hotspot in the field of biomaterials, with great market development space and potential, especially as bio-scaffold materials, ideal bio-scaffold materials require appropriate degradation rates and certain mechanical properties , but the shortcomings of traditional collagen-based scaffold materials such as: insufficient degradation resistance, weak mechanical properties, and difficulty in maintaining its inherent shape during application, thus limiting its application

[0003] At present, physical modification such as high temperature, ultraviolet rays, γ-rays, etc., chemical cross-linking by adding glutaraldehyde and other cross-linking agents or adjustment of process parameters are generally used to improve The mechanical strength of the collagen scaffold, but the above physical modification method can only slightly improve the mechanical properties of the collagen scaffold, which cannot meet the application requirements, and the chemical cross-linking is prone to cross-linking agent residue, resulting in biological toxicity, and adjusting the process parameters is likely to cause the collagen to be porous The pore size of the scaffold material is reduced, which is not conducive to cell proliferation and affects the induced tissue regeneration of the collagen scaffold

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