Hydrogel immobilized microorganism preservation method

Abstract

The invention discloses a hydrogel immobilized microorganism preservation method, and relates to the technical field of immobilized biologication. The hydrogel immobilized microorganism preservation method comprises the steps that microorganisms are immobilized in a hydrogel immobilized carrier through an embedding method, and storage and preservation are conducted at the normal temperature. The carrier is a composite gel ball, and comprises: an embedding filler, which comprises N-(1, 8-dimethylimidazo [1, 2-a] quinoxaline-4-yl)-1, 2-ethidene diamine modified chitosan microspheres, and a framework material which comprises sodium alginate, and the framework material and the embedding filler form an interpenetrating or semi-interpenetrating network structure. According to the microorganism preservation method provided by the invention, the hydrogel is used as a carrier and is embedded in the carrier through an immobilization technology, so that normal-temperature long-time storage, preservation and transportation can be realized; and the prepared composite gel ball carrier has good compressive strength, high porosity and good stability, and is more beneficial to microbial attachment.

Description

technical field [0001] The invention belongs to the technical field of immobilized biology, and in particular relates to a method for preserving microorganisms immobilized by hydrogel. Background technique [0002] Bacterial strains are important biological resources of the country and basic materials for production, teaching and scientific research. In recent years, more and more microbial strains have been studied and applied to various fields such as food, medicine, and agriculture. As the source of production, the quality of strains is directly related to the output and quality of products. In the field of microbiology, whether it is basic scientific research or applied research on biotechnology, correct strain preservation methods and technologies are required to ensure the quality and vitality of strains. Our country started relatively late in the field of strain preservation technology research, and has not yet formed a systematic operating procedure. The main proble...

AI Technical Summary

Problems solved by technology

Our country started relatively late in the field of strain preservation technology research, and has not yet formed a systematic operating procedure. The main problems are: the number of controlled tests is limited, the types of tests are small, and the research technical means need to be further updated. Insufficient research on the control of bacterial age, size of inoculated mycelium block, medium type, speed of cooling and freezing and warming and recovery, detection indicators, type of antifreeze protectant, etc.)

However, there are still problems. For example, even for the same species of Cordyceps militaris, different researchers use similar methods and draw different conclusions.

At the same time, liquid nitrogen cryopreservation and vacuum freeze-drying storage methods require good equipment and technology, and the investment is too high to be widely used in production; although the latter two methods are simple and cheap, they occupy a large storage space, and due to Too many tube transfers will increase the possibility of variation, which will easily cause strain degradation and bring losses to production

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