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Preparation method of physalis pubescens persistent calyx extract

A technology of physalis and extract, applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, can solve the problems of limited correlation research, and achieve the purpose of improving the purity, improving the inhibitory effect, and inducing apoptosis. Effect

Inactive Publication Date: 2021-07-30
JINZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research mainly focuses on the antitumor activity of withanolalide, and the correlation between a large number of chemical substances, especially flavonoids, and their efficacy is extremely limited. In the research, there are few studies on the extraction method of the persistent calyx extract of Physalis trichomes

Method used

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  • Preparation method of physalis pubescens persistent calyx extract
  • Preparation method of physalis pubescens persistent calyx extract
  • Preparation method of physalis pubescens persistent calyx extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A preparation method of Physalis edulis extract, comprising:

[0055] (1) Ultrasonic-assisted water-bath oscillation (UAWBSE) crude extraction, with flavonoids as evaluation indicators.

[0056] Physalis trichomes with calyx fruit in Jinzhou Fruit Wholesale Market. The fruit is separated from the persistent calyx, and the persistent calyx is oven-dried at 50°C to constant weight, crushed into powder by a pulverizer, sealed and stored in a -80°C refrigerator for later use. Take 14g of Physalis trichomes powder, add ethanol concentration 74% according to the solid-liquid ratio of 80mL / g, extract in a 30W power ultrasonic machine for 15min, then shake in a water bath at 50rpm at 65°C for 3.4h, filter and concentrate to 10mL, It is required that the flavonoid content of Physalis trichomes should reach 8.71±0.11mg / g DW.

[0057] According to the influence of solid-liquid ratio, the influence of ethanol concentration, the influence of ultrasonic time, the influence of extra...

Embodiment 2

[0093] Screening and composition of the ethyl acetate EAFPC "efficacy group" prepared in Example 1

[0094] Equipment required for experiment

[0095] Agilent 1209Infinity LC system and 6540 accurate-mass quadrupole time-of-flight mass spectrometry system (Accurate-mass Quadrupole Time-of-Flight (Q-TOF) MS system);

[0096] Chromatographic column: Agilent Zorbax Eclipse Plus C18 chromatographic column (2.1×50mm, 1.8μm), which is a high-performance silica gel-based chromatographic column upgraded by Agilent through silica gel manufacturing technology and bonding technology. The 1.8μm chromatographic column has the characteristics of high resolution and excellent compound identification ability.

[0097] HPLC-Q-TOF-MS / MS detection conditions

[0098] Mobile phase: water with 0.1% formic acid (A) and chromatographic grade acetonitrile (B).

[0099] Gradient conditions: Take B as an example, initially 15%, rise to 40% after 25 minutes, then rise to 95% after 10 minutes, keep fo...

Embodiment 3E

[0105] Example 3 The inhibitory effect of EAFPC on liver cancer cells

[0106] online databases used

[0107] PubChem database (https: / / pubchem.ncbi.nlm.nih.gov / )

[0108] Targetswiss (http: / / www.swisstargetprediction.ch / )

[0109] STINCH (http: / / stitch.embl.de / )

[0110] TCGA database (https: / / cancergenome.nih.gov / )

[0111] TTD (http: / / database.idrb.cqu.edu.cn / TTD / )

[0112] DrugBank database (http: / / www.drugbank.ca / )

[0113] OMIM database (http: / / www.omim.org / )

[0114] GAD (http: / / geneticassociationdb.nih.gov / )

[0115] PharmGKB (https: / / www.pharmgkb.org / index.jsp)

[0116] (1) Screening of EAFPC targets

[0117] The targets of the 16 components of EAFPC were predicted according to the principle of structural similarity, and the two databases of targetswiss and STINCH were used for joint prediction. The component structure information is obtained from the PubChem database, and the SMILES entry of the component is recorded, and then the SMILES is input into the Ta...

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Abstract

The invention discloses a preparation method of a physalis pubescens persistent calyx extract, which comprises the following steps: drying and crushing physalis pubescens persistent calyx to obtain physalis pubescens persistent calyx powder. The method comprises the following steps: adding ethanol into persistent calyx of physalis pubescens according to a material-to-liquid ratio of 20-100mL / g, carrying out ultrasonic extraction for 10-60 minutes, oscillating in a water bath at 60-80 DEG C for 3-5 hours, filtering and concentrating to obtain a crude extract of persistent calyx of physalis pubescens; adding the physalis pubescens persistent calyx crude extract into macroporous resin for adsorption and elution to obtain an eluent; and carrying out filtration, vacuum rotary concentration and freeze-drying on the eluent, dissolving the eluent in water, separating with petroleum ether for 2-4 times, extracting and separating with ethyl acetate for 3-7 times, and then carrying out filtration, concentration and vacuum drying to obtain the physalis pubescens persistent calyx extract. By performing two-step purification on the physalis pubescens persistent calyx crude extract, the purity of the extract is remarkably improved, and the inhibiting effect on liver cancer cells is further improved.

Description

technical field [0001] The invention relates to the technical field of plant extraction, and more specifically relates to a preparation method of the persistent calyx extract of Physalis trichomes. Background technique [0002] Physalis is rich in characteristic phytochemicals such as withanolide and a variety of significant physiological activities, so it has been widely concerned by the industries of medicine, food, cosmetics and so on. Among them, physalis is an annual herbaceous plant, also known as foreign girl, yellow mushroom mother, etc. It is a characteristic plant in Northeast China that has only attracted the attention of the pharmaceutical and food industries in recent years. Physalis edulis can not only be used as a good source of dietary nutrients, but also its anti-inflammatory, detoxification, anti-oxidation, anti-tumor and other health benefits are widely spread, but there is a lack of material basis research and modern scientific evidence. [0003] At pres...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P35/00A61P1/16
CPCA61K36/81A61K2236/333A61K2236/53A61K2236/55A61P1/16A61P35/00
Inventor 王晶晶
Owner JINZHOU MEDICAL UNIV