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Antibacterial silk fibroin and preparation method thereof

A technology of silk fibroin and antibacterial silk, which is applied in the field of antibacterial silk fibroin and its preparation, can solve the problems of application limitation, silk fibroin does not have antibacterial, antiviral and antitumor properties, and achieves short culture period and human safety. Harmless, low production cost effect

Inactive Publication Date: 2021-08-10
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, silk fibroin itself does not have antibacterial, antiviral and antitumor properties, and its application is limited.

Method used

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  • Antibacterial silk fibroin and preparation method thereof
  • Antibacterial silk fibroin and preparation method thereof
  • Antibacterial silk fibroin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 An antibacterial silk fibroin, which is obtained by loading actinomycin X2 on the silk fibroin through a dyeing process, and the specific preparation method is as follows:

[0028] Actinomycin X2 in this example is prepared from Streptomyces cyaneofuscatus isolated from the cyanobacterium Lyngbya sp. collected from the waters near Nanji Island, Wenzhou, through fermentation, extraction, purification, etc., with a purity of >99% %, the chemical structural formula is:

[0029]

[0030] , where the numbers indicate the positions of the carbon atoms.

[0031] (1) Put silkworm cocoon fragments into a container, add an appropriate amount of ultrapure water and place it in an ultrasonic cleaner, shake and wash at 100HZ for 5 minutes, take it out after washing and rinse with ultrapure water. Repeat 3 times, put into 60°C constant temperature drying oven for drying.

[0032] (2) Put the dried cocoons into 0.02M sodium carbonate solution, and degumming for 30-45...

Embodiment 2

[0051] The preparation of the antibacterial silk fibroin of embodiment 2 loaded actinomycin D:

[0052] Actinomycin D in this example was purchased from Nanjing Luomeimei Biotechnology Co., Ltd., with a purity of ≥98%, and its chemical structural formula is:

[0053]

[0054] According to o.w.f 0.1%, 0.25%, 1% and 2%, the dyeing combination experiment of actinomycin D and degummed silk was carried out, and the specific implementation steps were the same as in Example 1. By comparing the color of silk fibroin before and after dyeing, it is obvious that compared with pure silk fibroin, the dyed silk fibroin is yellow, indicating that actinomycin D has successfully combined with silk fibroin. As shown in Table 2, the K / S value of silk fibroin fibers combined with actinomycin D gradually increased with the increase of o.w.f ratio.

[0055] Table 2 Color intensity, colorimetric parameters and color difference (ΔE*) of actinomycin D-silk fibroin samples with different o.w.f rati...

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Abstract

The invention discloses antibacterial silk fibroin, actinomycin is loaded on the silk fibroin through a specific method, and the silk fibroin is functionalized by fully utilizing the wide biological activities of the actinomycin, such as anti-tumor, anti-bacterial, anti-virus and the like, so that the silk fibroin with high antibacterial property is prepared; the actinomycin has a good dyeing effect on the silk fibroin, the dye uptake is as high as 89.50%, and the actinomycin and the silk fibroin are firmly combined in a chemical mode, so that the prepared silk fibroin has stable antibacterial performance, and the silk fibroin combined with the actinomycin has an obvious inhibiting effect on staphylococcus aureus.

Description

technical field [0001] The invention relates to a silk fibroin, in particular to an antibacterial silk fibroin and a preparation method thereof. Background technique [0002] Silk fibroin (SF) is a natural polymer fibrin extracted from silk, which has superior mechanical properties, good cell compatibility, controllable biodegradability and low immunogenicity. These properties make it widely used in biomedical fields, such as wound dressings, tissue engineering, and sustained drug release. Therefore, silk fibroin is an ideal and safe biomedical material. However, silk fibroin itself does not have antibacterial, antiviral and antitumor properties, so its application is limited. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide an antibacterial silk fibroin with high antibacterial properties and a preparation method thereof. [0004] One of the technical solutions adopted by the present invention to solve the abov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K38/08A61P31/04A61L31/04A61L31/16
CPCA61K47/42A61K38/08A61P31/04A61L31/047A61L31/16C08L89/00
Inventor 韩兵男司冉冉陈佳宁陈薇谢宏宝
Owner ZHEJIANG SCI-TECH UNIV
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