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Preparation of a metal-organic framework composite and its application as a ratiometric fluorescent probe in the detection of hydrogen peroxide and pi

A metal-organic framework and composite material technology is applied in the field of preparation of metal-organic framework composite materials, which can solve the problems of expensive preparation, time-consuming, and easy deactivation.

Active Publication Date: 2022-05-03
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural enzymes have some disadvantages, such as easy inactivation in harsh chemical environments, expensive and time-consuming preparations

Method used

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  • Preparation of a metal-organic framework composite and its application as a ratiometric fluorescent probe in the detection of hydrogen peroxide and pi
  • Preparation of a metal-organic framework composite and its application as a ratiometric fluorescent probe in the detection of hydrogen peroxide and pi
  • Preparation of a metal-organic framework composite and its application as a ratiometric fluorescent probe in the detection of hydrogen peroxide and pi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of metal-organic framework composite material RhB@UiO-66(Zr / Fe)

[0036] (1) Take a 100 mL beaker, pipette 25 mL of DMF into the beaker with a graduated cylinder, then pipette 2.5 mL of glacial acetic acid into the beaker, then add 2.5 mM zirconium chloride and 2.5 mM ferric chloride into the beaker , sonicate until the zirconium chloride and ferric chloride dissolve.

[0037] (2) Take a 50 mL beaker, pipette 25 mL of DMF into the beaker, add 5 mM terephthalic acid and 50 mg RhB, and sonicate until dissolved.

[0038] (3) Transfer the solution in step (2) to the beaker in step (1), and stir for 30 minutes.

[0039] (4) Transfer the solution in step (3) into an 80 mL polytetrafluoroethylene autoclave, keep it warm at 120°C for 12 hours, then directly cool down, centrifuge at 8000 rpm for 5 minutes, and wash the solid powder with DMF After washing 3 times, and then washing 3 times with ethanol, the powder was collected by centrifugation, and vacuum...

Embodiment 2

[0040] Example 2 Metal-organic framework composites as ratiometric fluorescent probes for the detection of H 2 o 2

[0041] Add 20 μL NO to 20 μL RhB@UiO-66(Zr / Fe) acetic acid-sodium acetate buffer solution 3 - 、SCN - , S 2 o 3 2- , NO 2 - , F - , SO 3 2- , I - , Fe 2+ , Fe 3+ 、CN - and H 2 o 2 (1mM), if the fluorescence intensity ratio of RhB@UiO-66(Zr / Fe) in acetic acid-sodium acetate buffer solution I 573 / I 438 Significantly weakened, indicating that hydrogen peroxide was added; if the fluorescence intensity ratio I of the acetic acid-sodium acetate buffer solution of RhB@UiO-66(Zr / Fe) 573 / I 438 No significant change occurred, indicating that other ions were added.

Embodiment 3

[0042] Example 3 Metal-organic framework composites used as ratiometric fluorescent probes for continuous detection of Pi

[0043] In 20 μL RhB@UiO-66(Zr / Fe) acetic acid-sodium acetate buffer solution, first add 20 μL H 2 o 2 (1mM) to form a RhB@UiO-66-OH(Zr / Fe) solution, and then add 20 μL Ac - , SO 3 2- , SO 4 2- , NO 3 - 、Br - , Cl - 、Na + 、K + , Mn 2+ 、Al 3+ , Mg 2+ 、Co 2+ 、Cu 2+ and Pi (1mM), if the fluorescence intensity ratio of RhB@UiO-66-OH(Zr / Fe) solution I 573 / I 438 Significantly enhanced, indicating that Pi was added; if the fluorescence intensity ratio I of the RhB@UiO-66-OH(Zr / Fe) solution 573 / I 438 No significant change occurred, indicating that other ions were added.

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Abstract

The invention discloses a method for preparing a metal organic framework composite material, which comprises adding zirconium chloride, ferric chloride and glacial acetic acid into DMF, and ultrasonically dissolving to obtain a mixed solution of zirconium and iron; dissolving phthalic acid and rhodamine B in A mixed solution of phthalic acid and rhodamine B was obtained in DMF; the mixed solution of zirconium and iron was mixed with the mixed solution of phthalic acid and rhodamine B, stirred evenly, reacted at 100~120°C for 10~12 h, and then cooled , washed, and vacuum-dried to obtain the metal-organic framework composite RhB@UiO‑66 (Zr / Fe). h 2 o 2 Has a fluorescence-on effect on RhB@UiO‑66(Zr / Fe), and has a 2 o 2 On the basis of the fluorescence on, Pi has a fluorescence quenching effect on it, and the fluorescence on or fluorescence quenching is used as the response signal, while the fluorescence emission of RhB is not affected by the H 2 o 2 And the influence of Pi, use it as a stable reference signal, so as to achieve the H 2 o 2 The fluorescence ratio method detection of Pi and Pi has high sensitivity and strong selectivity, and can better avoid systematic errors and human errors, and has better reproducibility and stability.

Description

technical field [0001] The invention belongs to the technical field of chemical synthesis and fluorescent probes, and relates to a preparation method of a metal-organic framework composite material; 2 o 2 and applications in the Pi. Background technique [0002] Phosphate ion (Pi), an essential nutrient for phytoplankton and aquatic plants, is widely distributed in groundwater and stream water. Uncontrolled anthropogenic discharges, including agricultural fertilizer losses, industrial discharges, and domestic sewage discharges, pose a huge threat of Pi pollution to the aquatic environment. Excess phosphorus in lakes and rivers can cause a pollution phenomenon known as water eutrophication, which causes some aquatic plants to bloom and others to rot and die. Until now, efforts have been made to develop methods for the detection of Pi, including chromatographic, colorimetric, electroanalytical, and enzyme-based biosensors. Although these efforts have greatly advanced the f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C08G83/00G01N21/64
CPCC09K11/06C08G83/008G01N21/6428C09K2211/187C09K2211/183C09K2211/1088G01N2021/6432
Inventor 卢小泉阮晴贾元琪刘娟张蓉芳何耀荣王妮焦小梅白蕾韩振刚
Owner NORTHWEST NORMAL UNIVERSITY