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A sodium alginate/collagen composite bone scaffold and its preparation method and application

A sodium alginate and bone scaffold technology, applied in the field of regenerative medicine, can solve the problems of poor mechanical properties and unstable structure, and achieve the effects of high porosity, good biocompatibility, and easy attachment and proliferation.

Active Publication Date: 2022-08-09
SHANGHAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure collagen hydrogel bone scaffolds have the problems of poor mechanical properties and unstable structure.

Method used

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  • A sodium alginate/collagen composite bone scaffold and its preparation method and application
  • A sodium alginate/collagen composite bone scaffold and its preparation method and application
  • A sodium alginate/collagen composite bone scaffold and its preparation method and application

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preparation example Construction

[0032] The invention provides a preparation method of a sodium alginate / collagen composite bone scaffold, comprising the following steps:

[0033] Provide hydroxyapatite hollow microspheres loaded with antibacterial drugs;

[0034] Water, hydroxyapatite (HAP) nanoparticles, collagen cross-linking agent, collagen (Col), D-gluconolactone, sodium alginate (SA) and the antibacterial drug-loaded hydroxyapatite hollow microparticles were combined. The ball is subjected to a pre-crosslinking reaction to obtain a pre-crosslinking reaction mixture;

[0035] printing the pre-crosslinking reaction mixture to obtain a primary bone scaffold;

[0036] The primary bone scaffold and divalent copper ions are subjected to a re-crosslinking reaction to obtain the sodium alginate / collagen composite bone scaffold.

[0037] In the present invention, unless otherwise specified, the raw materials used in the present invention are preferably commercially available products.

[0038] The present inv...

Embodiment 1

[0084] Provides hydroxyapatite hollow microspheres loaded with antibacterial drugs:

[0085] (1) preparation of hydroxyapatite hollow microspheres, comprising the following steps:

[0086] 2.5g of polyaspartic acid and 200mL of 0.1mol / L sodium hydroxide solution were mixed in the first stage to obtain solution A;

[0087] Calcium nitrate tetrahydrate solution (2.365g calcium nitrate tetrahydrate dissolved in 100 mL of deionized water) was mixed with solution A in the second stage to obtain solution B;

[0088] The diammonium hydrogen phosphate solution (0.792 g of diammonium hydrogen phosphate dissolved in 100 mL of deionized water) is mixed with solution B in the third stage, and the mixing mode is constant magnetic stirring for 30 minutes to obtain a mixed solution;

[0089] Adjust the pH of the mixed solution to 5.0 with dilute nitric acid;

[0090] The mixed solution was transferred to a stainless steel autoclave lined with Teflon, heated at 180 °C for 3 h, centrifuged a...

Embodiment 2

[0102] The sodium alginate / collagen composite bone scaffold was prepared according to the method of Example 1, the only difference being:

[0103] The concentration of amoxicillin was 10 mg / mL.

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Abstract

The invention belongs to the technical field of regenerative medicine, and in particular relates to a sodium alginate / collagen composite bone support and a preparation method and application thereof, comprising the following steps: providing hydroxyapatite hollow microspheres loaded with antibacterial drugs; Limestone nanoparticles, collagen cross-linking agent, collagen, D-gluconolactone, sodium alginate and the antibacterial drug-loaded hydroxyapatite hollow microspheres are subjected to a pre-cross-linking reaction to obtain a pre-cross-linked mixture; The pre-crosslinking mixture is printed to obtain a primary bone scaffold; the primary bone scaffold and divalent copper ions are subjected to a re-crosslinking reaction to obtain the sodium alginate / collagen composite bone scaffold. The sodium alginate / collagen composite bone scaffold obtained by the preparation method of the present invention has good mechanical properties, relatively stable structure and antibacterial effect.

Description

technical field [0001] The invention belongs to the technical field of regenerative medicine, and in particular relates to a sodium alginate / collagen composite bone support and a preparation method and application thereof. Background technique [0002] Infected bone defect is an extremely serious complication, most often caused by trauma, tumor resection, and congenital diseases. For large-scale bone defects, there is no way for the body to fully heal itself, and in most cases, external surgical intervention is required to return to normal. Implant-related bone infections such as osteomyelitis frequently occur during bone trauma surgery, which hinders bone repair. Therefore, infected bone defects have always been a challenging clinical problem. [0003] Currently, methods of treating bone defects include autologous bone grafting, allogeneic bone grafting, and biomaterial scaffold grafting. Autologous bone transplantation has the disadvantages of insufficient bone supply a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/54A61L27/46A61L27/58A61L27/56
CPCA61L27/48A61L27/54A61L27/46A61L27/58A61L27/56A61L2430/02A61L2300/404A61L2300/622A61L2300/112C08L89/00C08L5/04
Inventor 张海光宋永腾胡庆夕王国印
Owner SHANGHAI UNIV
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