Preparation method of monodisperse magnetic polystyrene microspheres, microspheres and application thereof

A polystyrene microsphere and cross-linked polystyrene technology, which is applied in the preparation method of peptide, microsphere preparation, microcapsule preparation, etc., can solve the difficult process of magnetic polystyrene microsphere, magnetic polystyrene microsphere The problem of pellet agglomeration, affecting the magnetic application of materials, etc., achieves the effects of good dispersion, fast magnetic separation and uniform particle size.

Pending Publication Date: 2021-08-20
SUZHOU ENRICHING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the particle size requirements of ferric iron tetroxide nanoparticles and quantum dots (CdSe@ZnS) used in this method are 1-20nm and 1-10nm respectively. The process of making magnetic polystyrene microspheres is difficult
Moreover, the magnetic polystyrene microspheres prepared by this method are prone to magnetic flux leakage during use, which affects the magnetic properties of the material and subsequent further applications.
In addition, after the reaction is completed, the magnetic properties of the ferroferric oxide on the surface of the material will be changed after the adsorption and separation by the magnet. After the magnetic adsorption, the magnetic properties will be changed to paramagnetic, which will lead to the agglomeration of the magnetic polystyrene microspheres, which will affect the follow-up process. modification and application

Method used

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  • Preparation method of monodisperse magnetic polystyrene microspheres, microspheres and application thereof
  • Preparation method of monodisperse magnetic polystyrene microspheres, microspheres and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of monodisperse magnetic polystyrene microspheres, comprising the following steps:

[0029] S11 prepares seed microsphere:

[0030] Weigh 4g of polyvinylpyrrolidone PVP, dissolve it in 267ml of absolute ethanol and 133ml of ultrapure water, heat to 60°C, and stir for 25min;

[0031] In addition, measure 57ml of styrene and 267mg of AIBN and mix them evenly. After ultrasonic dissolution, add them to the above solution. Under the protection of nitrogen, heat up to 65°C and react for 24h. Wash the precipitate with absolute ethanol and ultrapure water, and obtain seed microspheres after drying;

[0032] The particle size of the seed microspheres is 800-1000nm;

[0033] S12 swelling polymerization to prepare porous cross-linked polystyrene microspheres.

[0034] Measure 20ml of divinylbenzene, 40ml of toluene, and 1ml of (2-ethylhexanoic acid) tert-butyl peroxide into a glass beaker, mix well, weigh 1g of PVP and dissolve it in 100ml of 0.45% SDS solu...

Embodiment 2

[0046] A preparation method of monodisperse magnetic polystyrene microspheres, comprising the following steps:

[0047] S21 prepares seed microspheres:

[0048] Weigh 5g of polyvinylpyrrolidone PVP, dissolve in 300ml of absolute ethanol and 100ml of ultrapure water, heat to 70°C, and stir for 30min;

[0049] In addition, measure 30ml of styrene and 300mg of AIBN and mix them evenly. After ultrasonic dissolution, add them to the above solution. Under the protection of nitrogen, heat up to 75°C and react for 24h. Wash the precipitate with absolute ethanol and ultrapure water, and obtain seed microspheres after drying;

[0050] The particle size of the seed microspheres is 800-1000nm;

[0051] S22 swelling polymerization to prepare porous cross-linked polystyrene microspheres.

[0052] Measure 20ml of divinylbenzene, 40ml of toluene, and 1ml of (2-ethylhexanoic acid) tert-butyl peroxide into a glass beaker, mix well, weigh 1.5g of PVP and dissolve it in 100ml of 0.5% SDS solut...

Embodiment 3

[0061] A preparation method of monodisperse magnetic polystyrene microspheres, comprising the following steps:

[0062] S31 prepares seed microsphere:

[0063] Weigh 5.2g of polyvinylpyrrolidone PVP, dissolve in 311ml of absolute ethanol and 89ml of ultrapure water, heat to 75°C, and stir for 35min;

[0064] In addition, measure 36ml of styrene and 305mg of AIBN and mix them evenly. After ultrasonic dissolution, add them to the above solution. Under the protection of nitrogen, heat up to 80°C and react for 24h. Wash the precipitate with absolute ethanol and ultrapure water, and obtain seed microspheres after drying;

[0065] The particle size of the seed microspheres is 800-1000nm;

[0066] S32 swelling polymerization to prepare porous cross-linked polystyrene microspheres.

[0067] Measure 20ml of divinylbenzene, 40ml of toluene, and 1ml of (2-ethylhexanoic acid) tert-butyl peroxide into a glass beaker, mix well, weigh 2g of PVP and dissolve it in 100ml of 0.6% SDS solutio...

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Abstract

The invention discloses a preparation method of monodisperse magnetic polystyrene microspheres, microspheres prepared by the preparation method and application of the microspheres. The preparation method comprises the following steps that: seed microspheres are prepared by a solution polymerization method; porous crosslinked polystyrene microspheres are prepared by swelling polymerization; nitro porous crosslinked polystyrene microspheres are prepared under acidic conditions; finally, through an in-situ reaction, the microspheres in the previous step is mixed with inorganic salt with ferrous ions, an initiator and ammonia water for reaction, and performing centrifugal cleaning to obtain the monodisperse magnetic polystyrene microspheres. In the preparation process, the usage amount of an organic reagent and the amount of wastes are less than those in the prior art; and the prepared magnetic polystyrene microspheres have the advantages of monodispersity, low sedimentation rate, high magnetic separation speed and mild separation conditions.

Description

technical field [0001] The invention belongs to the technical field of magnetic material synthesis, and in particular relates to a preparation method of monodisperse magnetic polystyrene microspheres, the microspheres and applications. Background technique [0002] In recent years, magnetic polystyrene microspheres have been widely used in the fields of magnetic particle chemiluminescence, immunodiagnosis, and NGS gene sequencing (fragmentation screening). [0003] The methods of monodisperse polystyrene microspheres mainly include dispersion polymerization, suspension polymerization, and seed multi-step swelling polymerization. Among them, the seed multi-step swelling polymerization method is to swell the polymer seed in the organic phase, and the particle size of the particles can be further controlled by controlling the amount of monomer added. The different particle sizes of polystyrene microspheres and whether they are magnetic also affect their subsequent application ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/40C08J9/36B01J13/02B01J13/14C07K1/14C12N15/10C08L25/06
CPCC08J9/40C08J9/365B01J13/14B01J13/02C12N15/1006C07K1/145C08J2325/06
Inventor 张佳斌张振宇尚春庆安新
Owner SUZHOU ENRICHING BIOTECH CO LTD
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