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Application of rubidium salt and rubidium salt solution in preparation of life-prolonging and/or anti-aging products

A life-prolonging and anti-aging technology, applied in the field of biomedicine, can solve problems such as unclear mechanisms, and achieve the effects of prolonging life and enhancing heat stress resistance

Active Publication Date: 2021-08-27
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2019, ZhengxiaoOuyang et al. reported (Frontiers In Pharmacology, 2019,(10):1–12.) that RbCl has an anti-osteoporosis effect, and its mechanism is still unclear

Method used

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  • Application of rubidium salt and rubidium salt solution in preparation of life-prolonging and/or anti-aging products
  • Application of rubidium salt and rubidium salt solution in preparation of life-prolonging and/or anti-aging products
  • Application of rubidium salt and rubidium salt solution in preparation of life-prolonging and/or anti-aging products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Application of Rubidium Chloride in Prolonging the Lifespan of Caenorhabditis elegans

[0046] (1) Strain preparation: Inoculate Escherichia coli (Escherichia coli) OP50 into LB liquid medium (4g peptone, 4g NaCl, 2g yeast extract, 4mL pH 8.0 1MTirs-HCl buffer solution, add ultrapure water to 400mL, high pressure steam sterilized), shake culture overnight and store in a 4°C refrigerator for later use.

[0047] (2) Preparation of NGM medium: add 1.2g NaCl, 6.8g agar, 1g peptone to a 500mL Erlenmeyer flask, add ultrapure water to 400mL, and sterilize with high pressure steam. When the solution is cooled to about 60°C, add 400 μl 1MCaCl on the ultra-clean bench 2 , 400 μl 5 mg / mL cholesterol, 400 μl 1MMgSO4, 10 mL 1M KHPO 4 Stir the buffer solution evenly and pour it into a Petri dish (35mm), let it solidify and store it in a 4°C refrigerator for later use.

[0048] (3) Seed plate: Use a pipette gun to absorb 200 μl of OP50 bacterial liquid in the center of th...

Embodiment 2

[0072] Example 2 Rubidium sulfate has no effect on the fecundity of Caenorhabditis elegans

[0073] Preparation of nematode strains, preparation of NGM medium, seeding plate, transfer of nematodes, subculture of nematodes, and synchronization of nematodes are as described in Example 1. Synchronized nematodes were placed on NGM medium (WT, 100 μM Rb 2 SO 4 , 10mM Rb 2 SO 4 ), which was recorded as Day 0 (Day 0), and cultured in a constant temperature incubator at 20° C. for 3 days. Adult nematodes were transferred to corresponding fresh NGM media (4 media per group, 1 nematode on each media). Cultivate in a constant temperature incubator at 20°C, count the number of eggs laid by nematodes every day, and transfer the nematodes to fresh NGM medium until the nematodes stop laying eggs.

[0074] Experimental results: In Caenorhabditis elegans, lifespan extension is often accompanied by a decrease in nematode fecundity. To investigate the changes in the number of eggs laid by ...

Embodiment 3

[0075] Example 3 Rubidium acetate enhances heat stress resistance of Caenorhabditis elegans

[0076] Strain preparation, NGM medium preparation, seeding plate, nematode transfer, nematode subculture, and nematode synchronization methods are as described in Example 1. Synchronized nematodes were placed on NGM medium (WT, 100 μM CH 3 COORb, 10mM CH 3 COORb), which is recorded as Day 0 (Day 0), and cultured in a constant temperature incubator at 20° C. for 3 days. Adult nematodes were transferred to corresponding groups of fresh NGM media (3 media for each group, 50 nematodes on each media), and the culture was continued until Day 6 (Day 6). The culture medium was transferred to a constant temperature incubator at 35°C, and the survival and death of nematodes were observed and counted every hour.

[0077] Experimental results: While the compound prolongs the lifespan of nematodes, it also tends to enhance the resistance of nematodes to various environmental stresses. To inves...

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Abstract

The invention discloses an application of rubidium salt and a rubidium salt solution in the preparation of life-prolonging and / or anti-aging products. The correlativity between rubidium salt and aging is confirmed through phenotypic experiments, the rubidium salt can activate DAF-16 through an AMPK pathway to prolong the service life of caenorhabditis elegans and enhance the heat stress resistance, ultraviolet radiation resistance and oxidative stress resistance, normal vital signs and reproductive capacity of the caenorhabditis elegans are not affected, and the rubidium salt is safe and free of toxic and side effects on the caenorhabditis elegans. Therefore, the rubidium salt and the rubidium salt solution have a good anti-aging effect and can be applied to preparation of anti-aging and life-prolonging products.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of rubidium salt and rubidium salt solution in the preparation of life-prolonging and / or anti-aging products. Background technique [0002] Aging is an inevitable part of the life process that can be delayed. Aging is characterized by decreased responsiveness to stress, increased homeostasis imbalances, and increased risk of age-related diseases, including cancer and heart disease. Aging is defined as "the progressive deterioration of physiological function, an age-related process of loss of vitality and increased vulnerability". Aging is a major contributor to the substantial increase in the incidence of chronic diseases such as dementia, cardiovascular disease, stroke, chronic respiratory disease, and cancer. These diseases are therefore also known as geriatric diseases and place a huge burden on the healthcare system. [0003] Lithium, sodium, potassium,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/16A23K20/20
CPCA23L33/16A23K20/30A23V2002/00A23V2200/302A23V2250/156
Inventor 徐峻郝梦娇张志康
Owner SUN YAT SEN UNIV
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