ULDPE-containing multi-layer co-extrusion disposable biotechnological bag membrane material and preparation method thereof

A one-time, membrane technology, applied in the directions of biochemical equipment and methods, chemical instruments and methods, bags, etc., can solve the problem of water resistance, bending resistance and puncture resistance. effect, the processing cost of bioprocess bags, etc., to achieve the effect of good biocompatibility, good cell culture effect, and easy operation

Inactive Publication Date: 2021-08-27
ZHEJIANG JINYISHENGSHI BIOENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the membrane materials of PALL, GE and CN201821441762.5 use nylon (PA) as its wear-resistant layer (outer layer). However, PA is not suitable for lap joint heat sealing welding process, which increases the processing cost of biotechnology bags. Production efficiency drops, PA is not suitable for use as the outermost heat-sealing layer
In addition, there are some patents related to cell culture composite membrane materials, but none of these membrane materials have a high gas barrier material layer
Therefore, the existing bioprocess bags are still difficult to obtain good gas barrier properties, and the water resistance, bending resistance, and puncture resistance are difficult to meet the needs, and also affect the cell culture effect and culture solution storage effect.

Method used

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  • ULDPE-containing multi-layer co-extrusion disposable biotechnological bag membrane material and preparation method thereof
  • ULDPE-containing multi-layer co-extrusion disposable biotechnological bag membrane material and preparation method thereof
  • ULDPE-containing multi-layer co-extrusion disposable biotechnological bag membrane material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1 adopts the film material for disposable biotechnology bags containing ULDPE prepared by co-extrusion blow molding method

[0062] In this example, the co-extrusion blow molding method is used to prepare a film material for a disposable biotechnology bag with a thickness of 250 μm. The composition of the film material includes the following figure 1 As shown, it includes heat seal layer 1, adhesive layer 2, gas barrier layer 3 / 4 / 5, adhesive layer 6 and liquid contact layer 7.

[0063] Among them, the heat-sealing layer LDPE adopts the Dow series of Dow, and the thickness of this layer is 50 μm.

[0064] The adhesive layers 2 and 6 are made of Dow Chemical Primacor resin with a thickness of 5 μm.

[0065] The gas barrier layers 3 and 5 are aromatic PA, which are made by American DuPont, and the thickness of the layers is 15 μm.

[0066] The gas barrier layer 4 is EVOH using Kuraray EVAL resin, and the thickness of this layer is 10 μm.

[0067] The ULDPE o...

Embodiment 2

[0079] Example 2 The membrane material for the disposable biotechnology bag containing ULDPE prepared by co-extrusion casting method

[0080] The composition and structure of the disposable bioprocess bag membrane material containing ULDPE provided by the present embodiment is as follows: figure 1 As shown, it includes heat seal layer 1, adhesive layer 2, gas barrier layer 3 / 4 / 5, adhesive layer 6 and liquid contact layer 7.

[0081] In this example, the co-extrusion casting method is used to prepare a disposable biotechnology bag film material with a thickness of 400 μm;

[0082] Heat-sealing layer 1 adopts the EVA of Celanese company, and the thickness of this layer is 100 μ m;

[0083] Adhesive layers 2 and 6 use Yangzi Petrochemical EVA with a thickness of 10 μm;

[0084] The gas barrier layers 3 and 5 on both sides are EVOH of the Kuraray EVAL series, with a thickness of 30 μm;

[0085] The intermediate gas barrier layer 4 is made of aromatic PA of the relevant brand o...

Embodiment 3

[0094] Embodiment 3 adopts the impact of different gas barrier layers on the performance of disposable bioprocess bags

[0095] In this example, the method provided in Example 2 is used to prepare a disposable biotechnology bag film material with a total thickness of 400 μm, and different gas barrier layers as shown in Table 5 are used respectively, where the thickness is the thickness of the gas barrier layer, The oxygen, water vapor permeability, tensile strength, transparency and cell culture effect (biocompatibility) of the prepared multilayer co-extruded disposable bioprocess bag membrane were investigated.

[0096] EVOH adopts Kuraray EVAL series, PA adopts aromatic PA, aliphatic PA, and aliphatic-aromatic PA of DuPont related brands in the United States, and PE uses related brands of Dow Company in the United States.

[0097] Among them, the oxygen, water vapor permeability, tensile strength, welding strength, transparency and cell culture effect detection methods are s...

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Abstract

The invention provides a ULDPE-containing multi-layer co-extrusion disposable biotechnological bag membrane material and a preparation method thereof. A nylon/ethylene-vinyl alcohol copolymer/nylon three-layer composite structure is adopted to prepare a gas barrier layer, ULDPE is used as a liquid contact layer, and an optimal heat sealing layer, an optimal bonding layer and an appropriate thickness are selected, so that the prepared disposable biological process bag has good gas barrier property, water resistance, strength, bending resistance, puncture resistance, excellent heat sealability and transparency, has good biocompatibility, high cell culture density and better cell culture effect, is simple in preparation process, simple and convenient to operate and high in efficiency, and can be used for preparing disposable bioreactor bags, liquid storage bags, stirring bags, weighing bags, feeding bags and the like.

Description

[0001] This application claims the priority of an earlier Chinese application, application number: 202110644601.6, with an application date of June 9, 2021; all its contents are regarded as a part of the present invention. technical field [0002] The invention belongs to the field of film materials for bioreactors, and in particular relates to a film material for multi-layer co-extruded disposable biotechnology bags containing ULDPE used in bioreactors and a preparation method thereof. Background technique [0003] Disposable biotechnology bags can be made into liquid storage bags, mixing bags, bioreactor bags, weighing bags, and feeding bags, etc., which are useful in the development and production of various types of cell suspension cultures, recombinant proteins, and other bioengineering products. Wide range of applications, no cleaning, disposable, high barrier and biocompatibility not only greatly improve work efficiency, but also avoid cross-contamination between some ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B32B27/08B32B27/34B32B27/30B32B27/32B32B7/12B29C48/00B29C48/10B29C48/14C12M3/00
CPCB32B27/34B32B27/32C12M23/14B32B27/40B32B27/12B32B5/02B29C55/28B29C41/32C12N5/0686C12N5/0603B32B2262/0261B32B2307/724B32B2250/24
Inventor 杨宇明徐江宁宋金沛
Owner ZHEJIANG JINYISHENGSHI BIOENG CO LTD
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