Preparation, identification method and application of dandelion polysaccharide and its selenium nanocomposite
A dandelion and selenium nanotechnology, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, to achieve the effect of green and simple preparation method, clear structure, and inhibition of tumor cell proliferation
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Embodiment 1
[0055] Embodiment 1 The preparation method of dandelion polysaccharide
[0056] The preparation method of described dandelion polysaccharide comprises the following steps:
[0057] S1 cutting: Cut 2kg of dry dandelion roots with scissors to 1-3cm, quickly wash with cold water, and dry to obtain dandelion root segments;
[0058] S2 water extraction: add 10 times its weight of water to the dandelion root segment obtained in step S1, heat to 80°C for extraction, extract for 3 hours, collect the extract, and dry the medicinal residues to obtain the extract and medicinal residues;
[0059] S3 graded alcohol precipitation:
[0060] After the extract obtained in step S2 was concentrated under reduced pressure at 60°C, ethanol was added until the volume concentration of ethanol was 50%, and after standing at room temperature for 24 hours, centrifugation was performed to collect the precipitate and supernatant to obtain crude polysaccharide TM1 and supernatant 1;
[0061] S4 purifica...
Embodiment 2
[0062] Example 2 Dandelion polysaccharide TM1-2
[0063] The dandelion polysaccharide TM1-2 is obtained by purifying the dandelion polysaccharide TM1 obtained in Example 1, and the specific method is as follows:
[0064] Ion-exchange column chromatography: Take 200 mg of dandelion polysaccharide TM1 obtained in Example 1, dissolve it in 5 mL of deionized water, and load it on a DEAE-FF column. Two peaks appear under the eluent conditions of different salt concentrations, and 0.05 M The elution peak of the NaCl elution part (using the phenol-sulfuric acid method to track the elution curve during the elution process, and collecting the sugar part according to the elution curve), the obtained eluent was concentrated, dialyzed (molecular weight cut-off was 3000 Da), frozen After drying, a polysaccharide TM1-2 was obtained.
Embodiment 3
[0065] Example 3 Preparation of dandelion polysaccharide-selenium nanocomposite TM1-2-Se
[0066] Take 108 mg of dandelion polysaccharide TM1-2 in Example 2 and dissolve it in 18 mL of ultrapure water, add 1 mL of 60 mM sodium selenite solution and 2 mL of 20 mg / mL Tween 80 solution, stir at room temperature for 2 h, and slowly add 2 mL dropwise. 240 mM ascorbic acid solution was stirred at room temperature for 24 h. After the reaction, the reaction solution was placed in a dialysis bag with a molecular cutoff of 1000 Da, and the ultrapure water was dialyzed for 48 hours, and the water was changed every 6 hours. .
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