Preparation, identification method and application of dandelion polysaccharide and its selenium nanocomposite

A dandelion and selenium nanotechnology, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, to achieve the effect of green and simple preparation method, clear structure, and inhibition of tumor cell proliferation

Active Publication Date: 2022-07-22
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on dandelion polysaccharides mainly focuses on the optimization of the extraction process of its crude polysaccharides and the screening of the activity of crude polysaccharides, and there are few research reports on the combination of refined polysaccharides and selenium nanoparticles and their anti-tumor activity.

Method used

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  • Preparation, identification method and application of dandelion polysaccharide and its selenium nanocomposite
  • Preparation, identification method and application of dandelion polysaccharide and its selenium nanocomposite
  • Preparation, identification method and application of dandelion polysaccharide and its selenium nanocomposite

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Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1 The preparation method of dandelion polysaccharide

[0056] The preparation method of described dandelion polysaccharide comprises the following steps:

[0057] S1 cutting: Cut 2kg of dry dandelion roots with scissors to 1-3cm, quickly wash with cold water, and dry to obtain dandelion root segments;

[0058] S2 water extraction: add 10 times its weight of water to the dandelion root segment obtained in step S1, heat to 80°C for extraction, extract for 3 hours, collect the extract, and dry the medicinal residues to obtain the extract and medicinal residues;

[0059] S3 graded alcohol precipitation:

[0060] After the extract obtained in step S2 was concentrated under reduced pressure at 60°C, ethanol was added until the volume concentration of ethanol was 50%, and after standing at room temperature for 24 hours, centrifugation was performed to collect the precipitate and supernatant to obtain crude polysaccharide TM1 and supernatant 1;

[0061] S4 purifica...

Embodiment 2

[0062] Example 2 Dandelion polysaccharide TM1-2

[0063] The dandelion polysaccharide TM1-2 is obtained by purifying the dandelion polysaccharide TM1 obtained in Example 1, and the specific method is as follows:

[0064] Ion-exchange column chromatography: Take 200 mg of dandelion polysaccharide TM1 obtained in Example 1, dissolve it in 5 mL of deionized water, and load it on a DEAE-FF column. Two peaks appear under the eluent conditions of different salt concentrations, and 0.05 M The elution peak of the NaCl elution part (using the phenol-sulfuric acid method to track the elution curve during the elution process, and collecting the sugar part according to the elution curve), the obtained eluent was concentrated, dialyzed (molecular weight cut-off was 3000 Da), frozen After drying, a polysaccharide TM1-2 was obtained.

Embodiment 3

[0065] Example 3 Preparation of dandelion polysaccharide-selenium nanocomposite TM1-2-Se

[0066] Take 108 mg of dandelion polysaccharide TM1-2 in Example 2 and dissolve it in 18 mL of ultrapure water, add 1 mL of 60 mM sodium selenite solution and 2 mL of 20 mg / mL Tween 80 solution, stir at room temperature for 2 h, and slowly add 2 mL dropwise. 240 mM ascorbic acid solution was stirred at room temperature for 24 h. After the reaction, the reaction solution was placed in a dialysis bag with a molecular cutoff of 1000 Da, and the ultrapure water was dialyzed for 48 hours, and the water was changed every 6 hours. .

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Abstract

The invention belongs to the technical field of medicine and health food, and particularly relates to a preparation method, identification method and application of a dandelion polysaccharide and a selenium nanocomposite thereof. The molecular weight range of the dandelion polysaccharide provided by the invention is 1000-100000Da. The preparation method of the invention is simple, the reaction conditions are mild, and large-scale production is possible; and the chemical structure of the obtained high-purity dandelion polysaccharide is identified, the structure is clarified, and the structure basis is provided for exploring its pharmacological activity mechanism. At the same time, the dandelion polysaccharide-selenium nanocomposite obtained by the present invention has high selenium content, uniformly dispersed particles, strong antitumor activity, and has potential application value in the field of medicine.

Description

technical field [0001] The invention belongs to the technical field of medicine and health food, and in particular relates to a preparation method, identification method and application of a dandelion polysaccharide and a selenium nanocomposite thereof. Background technique [0002] Selenium (Selenium, Se) is one of the eight essential trace elements for the human body. It has a variety of biological activities and plays an important role in preventing Keshan disease, cardiovascular disease and inhibiting tumor growth. Selenium is widely used in the food and pharmaceutical industries. application prospects. At present, the widely used selenium supplement method in the food and pharmaceutical industries is to add inorganic selenium supplements, such as sodium selenite, sodium hydrogen selenite, sodium selenate, etc. However, inorganic selenium has high toxicity, narrow range of activity and toxicity, relatively small minimum lethal dose, and low utilization rate. According ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61K33/04A61P35/00G01N21/3563G01N23/04G01N23/20G01N23/22G01N23/2273G01N24/08G01N27/00G01N30/02
CPCC08B37/0054C08B37/0003A61K33/04A61K31/715A61P35/00G01N30/02G01N21/3563G01N24/08G01N27/00G01N23/04G01N23/20G01N23/22G01N23/2273A61K2300/00
Inventor 郭远强许婧张少杰宋紫腾石丽娟周丽楠
Owner NANKAI UNIV
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