Method for recovering methane production performance of anaerobic fermentation acid inhibition system by using photosynthetic bacteria

A technology of anaerobic fermentation and photosynthetic bacteria, applied in microorganism-based methods, fermentation, bacteria, etc., can solve the problems of complex composition of microbial agents, narrow recovery range, long recovery time, etc., to improve sewage treatment efficiency and reproduction speed. Fast, quick release effect

Active Publication Date: 2021-08-27
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems of this method are: the operation steps are cumbersome, and the addition amount of strongly basic anion exchange resin in the anaerobic digestion reactor is difficult to determine
The problem with this method is that the composition of the microbial agent is complex, the ratio is cumbersome, the cultivation is difficult, and it is difficult to popularize and apply it on a large scale in actual engineering.
The problem with this method is that the recovery range is narrow (the lower limit of the recoverable pH is only 6.6), and the recovery time is long (it takes 30-45 days), which limits the further application of this method.

Method used

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  • Method for recovering methane production performance of anaerobic fermentation acid inhibition system by using photosynthetic bacteria
  • Method for recovering methane production performance of anaerobic fermentation acid inhibition system by using photosynthetic bacteria
  • Method for recovering methane production performance of anaerobic fermentation acid inhibition system by using photosynthetic bacteria

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Embodiment 1

[0034] The invention provides a method for utilizing photosynthetic bacteria to restore the methanogenic performance of the anaerobic fermentation acid inhibition system, the process flow chart is as follows figure 1 As shown, the specific implementation is as follows:

[0035] (1) Cultivation of photosynthetic bacteria: use the improved RCVBN medium to enrich and cultivate the photosynthetic bacteria purchased on the market. After about 7-10 days, a large number of microbial cells were obtained, centrifuged at 12000r for 1min, and the supernatant was removed. Obtain photosynthetic bacteria cells.

[0036] (2) Start the reactor and simulate acidification conditions: use the anaerobic tower sludge of a brewery sewage treatment station as inoculation sludge, use 500mL blue cap bottle as anaerobic batch reactor, inoculate artificial simulated wastewater, and use 1000 mg COD The low load of / L / d starts three groups of the same reactors, and the gas production rate and methane pro...

Embodiment 2

[0040] Cultivation of photosynthetic bacteria: Use the improved RCVBN medium to enrich and cultivate the photosynthetic bacteria purchased in the market. After about 7 days, a large number of microbial cells were obtained, centrifuged at 12000r for 1min, and the supernatant was removed to obtain photosynthetic bacteria cells .

[0041] Start the reactor to simulate acidification conditions: use the anaerobic tower sludge of a brewery sewage treatment station as the inoculation sludge, use 1000mL blue cap bottle as the anaerobic batch reactor, inoculate artificial simulated wastewater, and use 1200mg COD / L / d Three groups of the same reactors were started at a low load, and the gas production rate and methane production rate increased slowly. After about 18 days, the reactor reached a stable operating state, and the organic load was increased to 6000mg COD / L / d, such as figure 2 As shown in Figure 4, the pH of the reactor dropped sharply to about 6.0, the methane production rat...

Embodiment 3

[0045] Cultivation of photosynthetic bacteria: The photosynthetic bacteria purchased in the market were enriched and cultivated with beer wastewater. After about 10 days, a large number of microbial cells were obtained, centrifuged at 13000r for 1min, and the supernatant was removed to obtain photosynthetic bacteria cells.

[0046] Start the reactor to simulate acidification conditions: using anaerobic sludge from an industrial wastewater treatment station as inoculation sludge, three sets of the same UASB reactors were used to treat high-concentration organic wastewater. After about 18 days, the reactor reached a stable operating state. At this time, the organic load suddenly increases, such as Figure 2 ~ Figure 4 As shown, the pH of the reactor dropped sharply to about 6.0, the methane production rate decreased to 30%, the corresponding gas production also decreased significantly, and the system was obviously acidified.

[0047] Dosing photosynthetic bacteria for recovery: ...

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Abstract

The invention discloses a method for recovering methane production performance of an anaerobic fermentation acid inhibition system by utilizing photosynthetic bacteria, which comprises the following steps: taking the photosynthetic bacteria as a bacteria source, carrying out expanded culture and centrifugal treatment to obtain photosynthetic bacteria thalli, putting the photosynthetic bacteria thalli into an anaerobic fermentation reactor, and carrying out auxiliary illumination, and judging and recovering the normal gas production and methane production capability of the anaerobic fermentation acid inhibition system by detecting the change of indexes. According to the method, acid inhibition can be relieved within 12 days, normal fermentation performance can be recovered, the microbial community structure in the system cannot be greatly influenced, and the original microbial function in the system can be kept.

Description

technical field [0001] The invention belongs to the technical field of water treatment technology, and in particular relates to a method for recovering the methane production performance of an anaerobic fermentation acid inhibition system by using photosynthetic bacteria. Background technique [0002] As a mature wastewater treatment process, anaerobic fermentation has been widely used in wastewater treatment in food processing, textile chemical industry, paper printing and other industries. However, in the actual operation of the traditional anaerobic fermentation process, the accumulation of volatile fatty acid (VFA) is often caused by the excessive organic load of the system or the C / N imbalance in the influent, which in turn leads to the problem of acid inhibition, which limits the Further promotion and application of this process. [0003] China Patent No. 201210490706.1 is an application for "A Method for Alleviating the Acid Inhibition of the Methanogenic Phase of An...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12N1/20C02F11/04C02F3/34C02F3/28C12R1/01
CPCC12P5/023C12N1/20C02F3/28C02F3/34C02F11/04Y02E50/30
Inventor 黄津辉赵维鑫
Owner NANKAI UNIV
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