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Salt-tolerant alkali-resistant aerobic denitrifying bacterium and application thereof

An aerobic denitrification and bacterial technology, applied in the field of environmental microorganisms and water treatment, can solve the problems of denitrification efficiency reduction and other issues

Inactive Publication Date: 2021-09-03
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, studies have shown that the optimum pH for the enzyme activity of denitrifying bacteria is neutral or slightly alkaline, and the denitrification process is a process of continuous alkali production, and the currently screened strains will have reduced denitrification efficiency in a strong alkaline environment. Phenomenon

Method used

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  • Salt-tolerant alkali-resistant aerobic denitrifying bacterium and application thereof
  • Salt-tolerant alkali-resistant aerobic denitrifying bacterium and application thereof
  • Salt-tolerant alkali-resistant aerobic denitrifying bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Screening of strains resistant to salt and alkali and aerobic denitrification.

[0025] Specific steps are as follows:

[0026] 1) Prepare aerobic denitrifying bacteria enrichment medium: yeast extract 0.5g / L, peptone 0.5g / L, sodium chloride 30g / L, glucose 0.5g / L, starch 0.5g / L, sodium pyruvate 0.3 g / L, dipotassium hydrogen phosphate 0.3g / L, magnesium sulfate 0.05g / L, dissolve in deionized water, adjust the pH to 7, and steam sterilize at 121°C for 30min.

[0027] 2) Inoculate 10 mL of high-salt activated excess sludge (Wastewater Treatment Plant of Tianjin Dagu Chemical Industry Co., Ltd.) into a 250 mL Erlenmeyer flask containing 100 mL of the above-mentioned liquid medium, and culture on a shaking table at 30° C. and 140 r / min for 5 days. A total of three generations of culture and enrichment were carried out to obtain a mixed bacterial solution.

[0028] 3) Prepare BTB medium for the isolation of aerobic denitrifying bacteria: potassium nitrate 1g / L, sodium succin...

Embodiment 2

[0034] Denitrification experiment of strains under high salt (salinity: 3%) conditions

[0035] The specific implementation steps are as follows:

[0036] Inoculate the above-mentioned denitrifying bacterial strain MSD4 into 100mL liquid denitrifying DM medium (potassium nitrate 0.72g / L, sodium succinate 2.8g / L, sodium chloride 30g / L, dipotassium hydrogen phosphate 1g / L, seven Magnesium sulfate water 1g / L) in a 250mL Erlenmeyer flask, placed in a shaker at 30°C and 140r / min for culture, and an uninoculated blank medium was used as a blank control. After inoculation, take a small amount of bacterial liquid according to 0h, 24h, 48h, 72h, 96h and 120h, and a part of the samples are used to determine the optical density of the bacteria (OD 600 ), taking time as the abscissa, OD 600 The growth curve of the denitrifying bacteria is plotted on the ordinate, and the growth cycle of the strain is observed. The rest of the bacterial liquid was centrifuged at 10 000 rpm for 2 min, an...

Embodiment 3

[0041] Denitrification experiments of strains under different salinity conditions

[0042] The specific implementation steps are as follows:

[0043] The strain MSD4 was inoculated in a denitrified DM medium with a salinity (calculated as NaCl) of 3%, and pre-cultured in a shaker at 30°C and 140r / min. After the strain grows to the logarithmic growth phase, take 30mL of the bacterial liquid and insert it into fresh 300mL denitrification DM medium with a salinity (calculated as NaCl) of 0%, 3%, 5%, 7%, 10% and 12%. , the initial pH was set to 7, and the shaking culture was carried out in a shaker at 30°C and 140r / min. The culture medium that was not inoculated with bacterial solution was used as a blank control for experiments under the same conditions. A small amount of bacterial liquid was taken at 0 h and 120 h after inoculation, and a part of it was directly used to measure the optical density (OD) of the bacterial cells. 600 ), the rest was centrifuged at 10 000 rpm for ...

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Abstract

The invention discloses a salt-tolerant alkali-resistant aerobic denitrifying bacterium and application thereof, and relates to the technical field of environmental microorganisms and water treatment. The bacterial strain is called after Pseudomonas sp. MSD4. Through screening and separating, the salt-tolerant alkali-resistant aerobic denitrifying bacterium is obtained, the salt-tolerant range of the salt-tolerant alkali-resistant aerobic denitrifying bacterium is 0-10%, and the pH-resistant range of the salt-tolerant alkali-resistant aerobic denitrifying bacterium is 5-11. The bacterial strain has a wide application prospect in processes, including high-salinity waste water treatment, salt-alkali repairing and the like.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms and water treatment, in particular to a salt-resistant, alkali-resistant, aerobic denitrifying bacterium and its application. Background technique [0002] In recent years, with the rapid development of social economy, a large amount of high-nitrogen wastewater has been discharged into water bodies, causing eutrophication of water bodies. The removal of nitrogen in water has become an important environmental problem that needs to be solved urgently. The biological method has become the most environmentally friendly and economical treatment method because of its advantages of effective denitrification and no secondary pollution. The traditional biological denitrification process includes two processes of nitrification and denitrification. Among them, the denitrification bacteria are facultative anaerobic bacteria, while the nitrification bacteria are aerobic bacteria. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C09K17/14C12R1/38
CPCC02F3/34C02F3/02C09K17/14Y02W10/10
Inventor 黄津辉曾小英
Owner NANKAI UNIV
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