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Multifunctional magnetic control nano chain with biological catalysis effect

A nano-chain, magnetron technology, used in biochemical equipment and methods, determination/inspection of microorganisms, catalysts for physical/chemical processes, etc.

Active Publication Date: 2021-09-10
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the study of deep enhancement of biochemical reactions by integrating three-dimensional magnetic fields and simultaneous multiple catalytic mechanisms for different tasks by only one nanomaterial has not been reported yet.

Method used

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  • Multifunctional magnetic control nano chain with biological catalysis effect
  • Multifunctional magnetic control nano chain with biological catalysis effect
  • Multifunctional magnetic control nano chain with biological catalysis effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Synthesis of Materials

[0038] The magneto-control nano chain is a new material form due to its nano-rotor characteristics, strong modification / adsorption, and the characteristics of magnetic field response, in recent years have received more and more attention. The main reactants of its synthesis are tetrasoxide three iron nanospheres (both diameter 100-400 nm) and TEOS (orthodoxate), solvent is anhydrous ethanol, and the catalyst is concentrated water. For commonly used synthetic reactions, we added 50 mg of three-neck nanospheres and 60 ml of a three-necked iron flask and 60 ml of absolute anhydrous ethanol, and 3.5 ml of concentrated ammonia water was added, and the mechanical stirring 700 rpm was maintained for 20 min, so that ammonia was uniformly dispersed in the system. Further, a coordination is formed on the surface of the tetrasoxide. The speed is then lowered to 300 rpm, and 240-400 μl of TEOS is quickly added, and the reaction is 15-30 min, and the...

Embodiment 2

[0045] Example 2 Fe 3 O 4 @Sio 2 Community control of NanoChain Nano-chain Swirl

[0046] Compared with monomer control, group control is a more advanced concept - via magnetic field regulation makes a large number of magnetic particles exhibit a particular group arrangement, thereby having functions that can be achieved by robotic monomers. The structural regulation of the material and the parameter regulation of the magnetic field have an important impact on the form of group motion.

[0047] In our related research, Fengfeng is synthesized by magnetism synthesis. 3 O 4 @Sio 2 Nano chain. Under the action of the annular magnetic field, the nano-chain reflects the two interactions of "reunion-derived characterization" and "chain structure rotor vortex", exhibiting a larger "vortex" than a single nano-flap area. ". By regulating the magnetic field parameters, this scroll can be regulated by rotating speed, vortex size, drift, etc., that is, FE 3 O 4 @Sio 2 Population control of th...

Embodiment 3

[0053] Example 3 Fe 3 O 4 @Sio 2 NanoChain Nanoine for biological catalysis

[0054] The result of the group control is that the orientation of the chemical reaction system or the biological system is equivalent to coupled with "eyes, hands and brain", thereby possible for reaction catalysis. For example, we have studied Fe3O4 @ SiO2 nano chains, which generate vortex under magnetic field, which affects the surrounding flow fields, thereby accelerating spreading mass, increases the reaction rate, and acts as "nano-stirred child" Role. In addition, the ferroxide itself has the effect of promoting free radical production and transmission, so itself has a catalytic function - on the one hand, can interact with high frequency magnetic fields to promote reactions; on the other hand, ferroxation I have chemical catalytic capabilities. 3 mechanisms of physical stirring, high frequency magnetic fields and chemical reactions, making Fe 3 O 4 @Sio 2 The catalytic effect of the nano-chain is...

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Abstract

The invention provides a multifunctional magnetic control nano chain which is synthesized under the control of a three-dimensional magnetic field, can realize group movement and has a biological catalysis effect. The multifunctional magnetic control nano chain is composed of Fe3O4 nanospheres and SiO2. The invention also provides a synthesis control and function realization method. By controlling the interaction between reaction kinetics and a magnetic field in the synthesis process of the magnetic control nano chain, the nano chain with a controllable structure is obtained. Under the coupling of a rotating magnetic field and fluid mechanics, the group movement behavior of the nano chain can be analyzed and simulated, so that the assembly mechanism of the nano chain can be known, and principle support is provided for nano stirring. Three different mechanisms of the nano chain for biological catalysis are researched: a nano stirrer, a high-frequency magnetic field charge transfer medium and iron oxide chemical catalysis. The magnetic control nano chain shows an excellent reaction rate increasing effect when applied to 'glucose oxidase-horseradish peroxidase' and 'cell-free protein expression systems'.

Description

Technical field [0001] The present invention belongs to the field of biocatalytic, and more particularly to synthesis control and functional implementation of multi-functional magnetron nano-nano-chain of three-dimensional magnetic field control, a multifunctional magneto-controlled nano-nanchain having a biocatalytic effect. Background technique [0002] Magnetic response nanomaterials have extensive application prospects in the fields of drug active drug administration, magnetic resonance imaging, remote micro operation, catalysis, and controlled magnetic separation, and have received more and more research. Compared to zero-dimensional magnetic particles, a one-dimensional magnetic material such as nano-chain, nanorod, nanoire, etc. have unique anisotropy and adjustable structural properties, with more complex and diverse application prospects. In various methods of preparing one-dimensional magnetic nano chains, magnetic field-induced sol-gel method can promote the magnetic d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/745B01J35/08B01J37/03B01J37/34C12N15/70C12P21/00C12Q1/26C12Q1/28C12Q1/54
CPCB01J23/745B01J23/002B01J37/033B01J37/342C12Q1/54C12Q1/26C12Q1/28C12N15/70C12P21/00B01J35/33B01J35/23B01J35/51
Inventor 卢元刘东李尚松
Owner TSINGHUA UNIV
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