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Application of inactivated lactic acid bacteria compound in preparation of product for treating and/or preventing diabetes

A technology of lactic acid bacteria and compound, applied in the field of hypoglycemic, can solve the problems of complex production process of live bacteria preparation, inferior hypoglycemic effect and high cost

Pending Publication Date: 2021-09-14
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, live bacteria preparations are generally used in probiotic preparations, and the production process of live bacteria preparations is relatively complicated and the cost is high.
The inactivated lactic acid bacteria preparation has no biological activity, so its hypoglycemic effect is not as good as that of live bacteria preparations

Method used

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  • Application of inactivated lactic acid bacteria compound in preparation of product for treating and/or preventing diabetes
  • Application of inactivated lactic acid bacteria compound in preparation of product for treating and/or preventing diabetes
  • Application of inactivated lactic acid bacteria compound in preparation of product for treating and/or preventing diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of Inactivated Lactic Acid Bacteria Complex

[0035] MRS solid medium (both MRS broth medium and agar were purchased from Beijing Aoboxing Biotechnology Co., Ltd.) was sterilized at 121°C for 25 minutes, placed on a slant to cool to room temperature, lined aseptically in an ultra-clean workbench, and then Add Lactobacillus plantarum CICC 24202 (Lp) and Lactobacillus breve YM 1301Lp (Lb) cultured in glycerol, and culture in an incubator at 37°C for 24-48 hours, and repeat the activation 2-3 times. Sterilize the MRS liquid culture medium (250mL Erlenmeyer flask liquid volume is 50mL) in the Erlenmeyer flask at 115°C for 20min, cool to 30-40°C, pick the well-growth colonies on the slant culture medium, and inoculate Cultured in MRS liquid medium.

[0036] After culturing the liquid medium for 18 hours, mix Lp and Lb according to the volume ratio of 1:1, count them, and adjust to 10 with sterile normal saline. 8 CFU / mL, which is the oral live bacterial preparati...

Embodiment 2

[0039] Animal experiment

[0040] 50 male Kunming mice, weighing 18g±2g, were raised in a clean animal room, kept at a constant temperature of 24±1°C, with a relative humidity of 50%-70%, simulated light for 12h day and night cycles, standard experimental feed and free drinking water, and adapted to Experiments were started one week after sexual feeding. The mice were randomly divided into two groups: the normal control group (N.control) and the model group. The normal control group (10) was given ordinary feed, and the model group (40) was given high-fat diet (High Fat Diet, HFD). feed. After feeding with high-fat feed for 4 weeks from the second week, in addition to the normal control group, the model group was given multiple small doses of streptozotocin (STZ, purchased from Sigma, USA, 70 mg / kg, intraperitoneal injection) to induce Type 2 diabetes mellitus (T2DM) mouse model occurs, (STZ is dissolved in 0.1mol / L citric acid-sodium citrate buffer solution, pH = 4.4, is pr...

Embodiment 3

[0044] The fasting blood glucose of the type 2 diabetes model mice was ≥11.0mmol / L, which was considered as successful modeling. Relevant studies have shown that feeding high-fat diets will cause obesity, hyperinsulinemia and insulin resistance in mice, rather than obvious hyperglycemia or diabetes, and the injection of STZ will directly lead to the destruction of pancreatic β cells, resulting in insulin deficiency rather than insulin resistance Therefore, insulin resistance can be induced by high-fat diet, and then injected with low doses of STZ to damage islet function and cause blood sugar to rise, which can simulate the pathogenesis of type 2 diabetes.

[0045] The mice after successful modeling in Example 2 were randomly divided into 4 groups, namely the model group, the positive drug group, the live bacteria group and the inactivated bacteria group, and the treatment methods were the same as in Example 2. The fasting blood glucose was measured as the initial blood glucos...

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Abstract

The invention provides application of an inactivated lactic acid bacteria compound in preparation of a product for treating and / or preventing diabetes, and belongs to the technical field of blood sugar reduction. Lactobacillus plantarum CICC 24202 and lactobacillus brevis YM 1301 are mixed and inactivated, and in a type 2 diabetes model induced by combination of high-fat feed and multiple times of small-dose streptozotocin, the improvement degree of an inactivated bacteria group on type 2 diabetes can reach the level of a viable bacteria group; and the food intake and water intake of type 2 diabetes model mice can be reduced, the body weight of the mice can be increased to a certain extent, blood sugar can be effectively reduced, insulin sensitivity can be improved, and insulin resistance can be improved. Moreover, the inactivated bacteria obviously reduce fat accumulation of the mice with type 2 diabetes, improve renal dysfunction caused by diabetes to a certain extent, and can be applied to preparation of a product for treating and / or preventing diabetes.

Description

technical field [0001] The invention belongs to the technical field of blood sugar reduction, and in particular relates to the application of an inactivated lactic acid bacteria compound in preparing products for treating and / or preventing diabetes. Background technique [0002] With economic development and dietary enrichment, diabetes mellitus (DM) has become the third major non-communicable disease that endangers human health. According to the latest data from the Global Diabetes Overview (Ninth Edition) provided by the International Diabetes Federation, as of 2019, approximately 463 million adults worldwide suffer from diabetes, of which 116.4 million are adults in China. [0003] Diabetes mellitus is a chronic disease in which lipid and glucose metabolism in the body are disturbed due to absolute or relative insufficiency of insulin in the body, or decreased sensitivity of peripheral tissues to insulin. According to its etiology and mechanism, it can be divided into typ...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P3/10A61P5/50A61P13/12A61P3/06A23L33/135
CPCA61K35/747A61P3/10A61P5/50A61P13/12A61P3/06A23L33/135A23V2002/00A61K2300/00A23V2200/3262A23V2200/328A23V2250/206
Inventor 李发荣黄春英薛楠魏倩倩周蓉存党媛婕高婧茹万娟周杨晶张宝善
Owner SHAANXI NORMAL UNIV
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