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Microalgae preservative and application thereof

A preservative, microalgae technology, applied in microorganisms, preservation microorganisms, unicellular algae and other directions, can solve the problems of easy apoptosis, difficult preservation and transportation, corruption and so on

Pending Publication Date: 2021-09-14
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microalgae antistaling agent provided by the present invention has a good freshness-preserving effect on microalgae, and more than 90% of the vitality of chlorella can be kept at room temperature for more than 3 months, which effectively solves the problem of easy apoptosis, corruption, and difficulty in preservation of algae when stored at room temperature. Transportation and other issues

Method used

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  • Microalgae preservative and application thereof
  • Microalgae preservative and application thereof
  • Microalgae preservative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 Microalgae preservative of the present invention

[0042] Material A: Trichoderma multispora solution

[0043] Material B: Pichia pastoris liquid

[0044] Material C: Streptococcus lactis extract

[0045] D material: Pulilan polysaccharide aqueous solution (mass fraction is 20-30%)

[0046] E material: radish polysaccharide aqueous solution (mass fraction is 20-30% 20-30% mass concentration)

[0047] A material:

[0048] Trichoderma multispora was inoculated into the culture medium according to 5% ratio (V:V) (sodium nitrate 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sodium chloride 60g , 30g of sucrose, 1L of purified water, fully dissolved and then sterilized at 118°C for 30 minutes), cultured aseptically at 25-30°C for 48 hours, and centrifuged in a centrifuge (6000-10000 rpm, 5-10 minutes) , and centrifuge to get the supernatant, which is Trichoderma multispora culture solution.

...

Embodiment 2

[0054] Embodiment 2 Microalgae preservative of the present invention

[0055] Material A: Trichoderma multispora solution

[0056] Material B: Pichia pastoris liquid

[0057] Material C: Streptococcus lactis extract

[0058] D material: Pulilan polysaccharide aqueous solution (mass fraction is 20-30%)

[0059] E material: radish polysaccharide aqueous solution (mass fraction is 20-30% 20-30% mass concentration)

[0060] A material:

[0061] Trichoderma multispora was inoculated into the culture medium according to 5% ratio (V:V) (sodium nitrate 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sodium chloride 60g , 30g of sucrose, 1L of purified water, fully dissolved and then sterilized at 118°C for 30 minutes), cultured aseptically at 25-30°C for 48 hours, and centrifuged in a centrifuge (6000-10000 rpm, 5-10 minutes) , and centrifuge to get the supernatant, which is Trichoderma multispora culture solution.

...

Embodiment 3

[0067] Embodiment 3 Fresh chlorella algae are stored at room temperature

[0068] With freshly collected seawater chlorella (algae body density 500-2000 / ml), add 2% microalgae antistaling agent of embodiment 1 in every liter of algae liquid, and be placed in dark and store at room temperature, weekly The algae density, vigor, color, miscellaneous bacteria content, and properties of the algae liquid were measured. The results are shown in Table 1.

[0069] The experimental group added the microalgae preservative of Comparative Example 1, and the other preservation techniques were the same as above.

[0070] Contrast group 1 without adding the microalgae preservative of embodiment 1, and other preservation techniques are the same as above.

[0071] Compared with the addition of 0.5% sodium nitrite (mass concentration) preservative in the two groups, other preservation processes are the same as above.

[0072] Table 1

[0073]

[0074] It can be seen that after adding the m...

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Abstract

The invention relates to the technical field of microalgae, in particular to a microalgae preservative, and a preparation method and application thereof. The microalgae preservative is prepared from a trichoderma polysporum culture solution, a pichia pastoris fermentation solution, a lactic streptococci fermentation solution, Pleilan polysaccharide and Pulullan. The microalgae preservative disclosed by the invention has obviously better effects on the aspects of keeping the activity of microalgae cells and inhibiting bacteria than a formula-lacking contrast, so that the components in the microalgae preservative disclosed by the invention have a mutual promotion effect, complement each other, generate a synergistic effect in microalgae preservation, and are indispensable.

Description

technical field [0001] The invention relates to the technical field of microalgae, in particular to a microalgae fresh-keeping agent and its application. Background technique [0002] Seawater chlorella has potential application value in many fields such as food and feed, medicine and health care, cosmetics and biomass energy, and biological pollution is one of the bottleneck problems restricting the large-scale cultivation of seawater chlorella, among which algae-eating zooplankton most serious hazard. At present, domestic and foreign research on this aspect is in its infancy, and most of the research is still on the screening of simple control methods, lacking an in-depth understanding of the relationship between zooplankton and marine chlorella. Seawater chlorella cells are rich in protein, vitamins and chlorophyll, and have high nutritional and economic value. It has been widely used in pharmaceutical industry, cosmetic industry and animal feed industry. Recent studie...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/04C12R1/89
CPCC12N1/12C12N1/04
Inventor 叶蕾黄成潭潘军黄敏
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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