Microalgae preservative and application thereof
A preservative, microalgae technology, applied in microorganisms, preservation microorganisms, unicellular algae and other directions, can solve the problems of easy apoptosis, difficult preservation and transportation, corruption and so on
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Embodiment 1
[0041] Embodiment 1 Microalgae preservative of the present invention
[0042] Material A: Trichoderma multispora solution
[0043] Material B: Pichia pastoris liquid
[0044] Material C: Streptococcus lactis extract
[0045] D material: Pulilan polysaccharide aqueous solution (mass fraction is 20-30%)
[0046] E material: radish polysaccharide aqueous solution (mass fraction is 20-30% 20-30% mass concentration)
[0047] A material:
[0048] Trichoderma multispora was inoculated into the culture medium according to 5% ratio (V:V) (sodium nitrate 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sodium chloride 60g , 30g of sucrose, 1L of purified water, fully dissolved and then sterilized at 118°C for 30 minutes), cultured aseptically at 25-30°C for 48 hours, and centrifuged in a centrifuge (6000-10000 rpm, 5-10 minutes) , and centrifuge to get the supernatant, which is Trichoderma multispora culture solution.
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Embodiment 2
[0054] Embodiment 2 Microalgae preservative of the present invention
[0055] Material A: Trichoderma multispora solution
[0056] Material B: Pichia pastoris liquid
[0057] Material C: Streptococcus lactis extract
[0058] D material: Pulilan polysaccharide aqueous solution (mass fraction is 20-30%)
[0059] E material: radish polysaccharide aqueous solution (mass fraction is 20-30% 20-30% mass concentration)
[0060] A material:
[0061] Trichoderma multispora was inoculated into the culture medium according to 5% ratio (V:V) (sodium nitrate 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sodium chloride 60g , 30g of sucrose, 1L of purified water, fully dissolved and then sterilized at 118°C for 30 minutes), cultured aseptically at 25-30°C for 48 hours, and centrifuged in a centrifuge (6000-10000 rpm, 5-10 minutes) , and centrifuge to get the supernatant, which is Trichoderma multispora culture solution.
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Embodiment 3
[0067] Embodiment 3 Fresh chlorella algae are stored at room temperature
[0068] With freshly collected seawater chlorella (algae body density 500-2000 / ml), add 2% microalgae antistaling agent of embodiment 1 in every liter of algae liquid, and be placed in dark and store at room temperature, weekly The algae density, vigor, color, miscellaneous bacteria content, and properties of the algae liquid were measured. The results are shown in Table 1.
[0069] The experimental group added the microalgae preservative of Comparative Example 1, and the other preservation techniques were the same as above.
[0070] Contrast group 1 without adding the microalgae preservative of embodiment 1, and other preservation techniques are the same as above.
[0071] Compared with the addition of 0.5% sodium nitrite (mass concentration) preservative in the two groups, other preservation processes are the same as above.
[0072] Table 1
[0073]
[0074] It can be seen that after adding the m...
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