Culture type virus preserving fluid capable of being sterilized by irradiation

Abstract

The invention relates to a culture type virus preserving fluid capable of being sterilized by irradiation. The culture type virus preserving fluid mainly comprises the following main components of sodium chloride, magnesium sulfate, potassium chloride, magnesium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, 4-hydroxyethyl piperazine ethanesulfonic acid, arginine, cysteine, glutamine, glycine, histidine, isoleucine, leucine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine, glucose, sucrose, vitamin C, vitamin E, amphotericin B, gentamicin, streptomycin, penicillin and phenol red. The preserving fluid can maintain the phenol red indicator to be red under the irradiation intensity as high as 20kGy, and plays a role of a pH indicator after subsequent sampling; and the preserving fluid can be used for directly preserving virions collected in throat swabs, nose swabs or specific part tissue samples, and is used for subsequent culture or nucleic acid extraction experiments.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a cultured virus preservation solution that can be sterilized by radiation. Background technique [0002] At present, the most important detection method for SARS-CoV-2 infection is nucleic acid detection. Real-time fluorescent RT-PCR technology or reverse transcription-polymerase chain reaction is mostly used clinically, and the samples used are nasopharyngeal swabs, sputum, alveolar lavage fluid and feces of infected patients, all of which contain SARS-CoV- 2 specific RNA sequences. In some scenarios, medical staff also need to isolate, cultivate virus particles, and conduct further experiments such as identification. If you need to culture virus, you must choose a non-inactivated virus preservation solution, such as Hanks solution, PBS buffer, saline, etc. However, traditional Hanks and PBS buffers can only play a buffering role and do not provide nutrients to the cells...

AI Technical Summary

Problems solved by technology

However, cultivating the virus preservation solution in the 10,000-class clean room is very prone to bacterial contamination

Not only that, if irradiation sterilization is carried out immediately after filling, although the effect of sterilization is achieved, high-intensity irradiation (higher than 10kGy) will cause the phenol red in the culture solution to lose its color, thus losing the instant detection of pH Ability

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