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A kind of hydroxyapatite nanorod with surface grafted egcg and its preparation method and application

A technology of hydroxyapatite and surface grafting, which is applied in the field of nanomaterials, can solve the problems of limited clinical application and poor bioavailability, and achieve the effects of increasing the application range, supporting bone growth, and improving stability

Active Publication Date: 2022-07-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although EGCG has great anticancer potential, its poor bioavailability limits its clinical application

Method used

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  • A kind of hydroxyapatite nanorod with surface grafted egcg and its preparation method and application
  • A kind of hydroxyapatite nanorod with surface grafted egcg and its preparation method and application
  • A kind of hydroxyapatite nanorod with surface grafted egcg and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of a kind of hydroxyapatite surface grafting EGCG of the present embodiment, the specific preparation steps are as follows:

[0037] (1) Octadecylamine (1.0 g, 2.0 g, 3 g, 4 g, 6 g, 12 g) was dissolved in oleic acid (12 ml), and the heating temperature was 100°C. Add 48ml ethanol and stir to mix, then add 25ml Ca(NO 3 ) 2 .4H 2 O (0.25mol / L) solution, after stirring for 10min, add 25ml Na 3 PO 4 ·12H 2 O (0.15mol / L) aqueous solution, stirred for about 10min. Then transfer to a 150ml polytetrafluoroethylene hydrothermal reactor, seal the reactor, react at 150°C for 10h, and cool the autoclave to room temperature. Centrifuged, washed several times with cyclohexane, ethanol and water, and dried to obtain samples OA-OM-HA-1, OA-OM-HA-2, OA-OM-HA-3, OA-OM-HA-4, respectively , OA-OM-HA-6, OA-OM-HA-12, select the appropriate size OA-OM-HA-4 for subsequent experiments.

[0038] (2) Disperse 50 mg of OA-OM-HA-4 nanorods in 20 mL of cyclohexane, and...

Embodiment 2

[0042] The preparation method of a kind of hydroxyapatite surface grafting EGCG of the present embodiment, the specific preparation steps are as follows:

[0043] (1) The preparation of OA-OM-HA is the same as that of Example 2.

[0044] (2) Disperse 50 mg of OA-OM-HA-4 nanorods in 20 mL of acetone, and add phosphoethanolamine solution (50 mg in 20 mL of deionized water) in an ice bath with vigorous stirring at 1000 r / min, and react for 12 h. Centrifuged, washed three times with deionized water, and dried to obtain the sample HA-NH 2 .

[0045] (3) EGCG (0.05 mM) was dissolved in 3 ml of dry DMSO and added to a single-necked flask. Next, 1,1'-carbonyldiimidazole (0.05 mM) was added and the reaction was allowed to 2 Atmosphere and stirring in the dark for 24h. Then, the HA-NH 2 (20 mg) was added to the above solution and the reaction was allowed to proceed in the dark at room temperature for 24 h. After centrifugation, the product was washed several times with DMSO and ab...

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Abstract

The invention belongs to the technical field of nanomaterials, and discloses a hydroxyapatite nanorod with surface grafted EGCG and a preparation method and application thereof. Firstly, oleic acid and octadecylamine were used as templates to synthesize monodisperse hydroxyapatite with controllable size, and its surface was aminated with phosphoethanolamine, and EGCG was grafted with the amino groups on the surface through amide bonds. The hydroxyapatite-grafted EGCG nanomaterials prepared by this method can induce osteosarcoma cell apoptosis, and have the potential to repair bone defects and support bone growth.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and particularly relates to a preparation method and application of EGCG grafted on the surface of hydroxyapatite. Background technique [0002] Osteosarcoma is difficult to remove, and there is a high possibility of bone cancer recurrence after resection. It is of great significance to develop a bone repair material that can not only repair bone defects caused by tumor resection, but also better inhibit the proliferation of osteosarcoma cells in the surgical resection site, which can speed up the healing process of bone defects and reduce the risk of cancer recurrence. [0003] Hydroxyapatite (HA) is an inorganic component of bone, and research on using HA as a bone repair material has always been a frontier focus in the field of medical materials research. Epigallocatechin gallate (Epigallocatechin gallate, EGCG) is the most abundant and medicinally active ingredient in catechins, and ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/50A61L27/54C01B25/32B82Y30/00B82Y40/00
CPCA61L27/12A61L27/50A61L27/54C01B25/327B82Y30/00B82Y40/00A61L2430/02A61L2400/12A61L2300/412A61L2300/416A61L2300/216C01P2004/16C01P2004/04C01P2002/72C01P2002/82
Inventor 邓春林任俭周烁烁
Owner SOUTH CHINA UNIV OF TECH
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