Method for detecting cronobacter in infant formula milk powder

A Cronobacter, infant formula technology, applied in biochemical equipment and methods, microbial determination/inspection, measurement devices, etc., to save economic and labor costs, time-consuming, and low-cost effects

Active Publication Date: 2021-09-21
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the defects of existing detection methods and achieve rapid and accurate detection of Cron

Method used

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  • Method for detecting cronobacter in infant formula milk powder
  • Method for detecting cronobacter in infant formula milk powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Configure different concentrations of Cronobacter bacteria liquid (10 0 -10 2 cfu / mL), were inoculated into sterilized milk powder (100g), and stored at room temperature for 30 days, 60 days, and 90 days, and were isolated and identified according to the method of the present invention and the method of GB4789.40-2016. The results are shown in Table 1.

[0049]

[0050] The results show that the artificial simulated milk powder samples with different concentrations of pollution are likely to cause false negatives by using the GB4789.40-2016 method, while the enrichment medium of the present invention can well overcome the damage to bacteria during dry storage, even if the pollution level is low, Compared with the GB4789.40-2016 method, the detection rate of Cronobacter has better accuracy and enrichment effect, and its sensitivity is higher than that of the GB4789.40-2016 method.

Embodiment 2

[0052] Configure different concentrations of Cronobacter bacteria liquid (2 × 10 0 -2×10 2 cfu / mL) and a fixed concentration of Escherichia coli (2×10 5 cfu / mL), Salmonella (2×10 5 cfu / mL), Staphylococcus aureus (2×10 5 cfu / mL), prepared as 10 0 cfu / mL Cronobacter-10 5 cfu / mL (E. coli or Salmonella or Staphylococcus aureus), 10 1 cfu / mL Cronobacter-10 5 cfu / mL (E. coli or Salmonella or Staphylococcus aureus), 10 2 cfu / mL Cronobacter-10 5 Cfu / mL (Escherichia coli or Salmonella or Staphylococcus aureus) combined simulated samples were inoculated into sterilized milk powder (100 g), stored at room temperature for 90 days, and separated according to the method of the present invention and the method of GB4789.40-2016 Identification, the results are shown in Table 2.

[0053]

[0054] The results show that the method of the present invention can well overcome the interference of common miscellaneous bacteria in milk powder such as Escherichia coli, Salmonella ...

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Abstract

The invention relates to a method for detecting cronobacter in infant formula milk powder, and belongs to the technical field of detection of biological products. The innovation lies in improvement of enrichment and separation of cronobacter in infant formula milk powder. A liquid culture medium (dry damage repair factors comprise 0.4-0.8 g/L of mannitol and 2.5-5.0 g/L of trehalose, and selective enrichment factors comprise 80-150 g/L of sucrose, 4-8 g/L of vancomycin and 0.3-0.6 g/L of sodium deoxycholate) is adopted for efficient enrichment, and a solid culture medium (selective components comprise 0.5-1.0 g/L of sodium deoxycholate and 80-100 mg/L of 5-bromo-4-chloro-3-indole-D-alpha-glucoside) is adopted for selective separation. The method has the advantages of high enrichment efficiency, strong selectivity, convenience in operation, short time and the like, and overcomes the defects of long time, low enrichment effect, complicated operation and the like of the existing detection method. The method is suitable for detecting cronobacter in formula milk powder, shortens the detection time, improves the detection accuracy and efficiency, reduces the detection cost, and has important significance in guaranteeing the safety of infant formula milk powder.

Description

technical field [0001] The invention belongs to the technical field of detection of biological products, and relates to a detection method of Cronobacter in formula milk powder for infants and young children. Background technique [0002] Cronobacter is an important food-borne pathogen that can cause meningitis, fatal colitis, and bacteremia in newborn infants. Epidemiological data surveys show that infant formula milk powder is the main vector of infection , so Cronobacter is a must-test item in infant formula milk powder. [0003] At present, my country's national food safety standard GB4789.40-2016 and the United States Food and Drug Administration (FDA) test the detection method of Cronobacter in infant formula milk powder. The selective isolation and biochemical identification of Cronobacter and solid medium will take about 6-8 days. Studies have found that media such as VRBGA and EE recommended by the FDA have poor selectivity and cannot effectively screen out Cronoba...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/04C12Q1/045G01N2333/195Y02A50/30C12Q1/10
Inventor 叶应旺凌娜蒋秀婷董晶晶王洋
Owner HEFEI UNIV OF TECH
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