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Hydrogel loaded with photosynthetic bacteria as well as preparation method and application of hydrogel

A technology of photosynthetic bacteria and hydrogel, applied in medical science, bandages, etc., can solve the problems of non-bacterial wound healing, poor barrier effect, etc., and achieve the effect of favorable healing, high similarity, and improved wound healing process

Active Publication Date: 2021-09-24
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a hydrogel loaded with photosynthetic bacteria and its preparation method and application, so as to solve the problems of poor barrier effect, no sterilization and wound healing promotion of traditional dressing materials

Method used

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  • Hydrogel loaded with photosynthetic bacteria as well as preparation method and application of hydrogel
  • Hydrogel loaded with photosynthetic bacteria as well as preparation method and application of hydrogel
  • Hydrogel loaded with photosynthetic bacteria as well as preparation method and application of hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The present invention has prepared the biological hydrogel dressing of carrying photosynthetic bacteria, comprises the following steps:

[0040] (1) Preparation of acellular porcine dermal matrix powder

[0041] a. Preparation of acellular dermal matrix

[0042] Take the dorsolateral full-thickness skin of a pig with a weight of about 120kg from the market, and soak the sample in distilled water for 2 hours. All samples were peeled to remove subcutaneous fat, connective tissue, and epidermis, and then dermal sections were decellularized. The specific steps of the decellularization protocol: process the dermal slices with 0.25% trypsin / 1% Triton X-100 decellularization protocol, and incubate at room temperature on a vortex shaker at 300 rpm, the conditions are: 0.25% trypsin incubation 6h, once; deionized water, 15min, three times; 70% ethanol, 11h, once; 3% hydrogen peroxide, 15min, once; deionized water, 15min, twice; 1% TritonX-100 at 0.26% EDTA / 0.69% Tris, 6h, onc...

Embodiment 2

[0060] The properties of the hydrogel were characterized as follows:

[0061] (1) Add the acellular matrix powder prepared in step (1) of Example 1 into three times of water to obtain different concentrations of unloaded hydrogels (see figure 1 shown), put the gel at -80°C for freezing, take it out and place it at room temperature (a is inverted, b is oblique) for a period of time, and observe the shape of the gel. The results are as follows figure 1 As shown, too much or too little hydrogel concentration will affect the gel morphology. In the end, only the hydrogels with concentrations of 15mg / ml and 20mg / ml had good morphology, and the optimal concentration was selected as the material for subsequent experiments. Take pictures of the 20mg / ml concentration hydrogel, the results are as follows figure 2 As shown, 20mg / ml has a more excellent form, so as to be determined as the subsequent experimental concentration.

[0062] (2) Ultraviolet measurement of unloaded hydrogel (...

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Abstract

The invention provides hydrogel loaded with photosynthetic bacteria as well as a preparation method and application of the hydrogel. The hydrogel is bacterium-carrying hydrogel formed by loading photosynthetic bacteria in an acellular matrix. The acellular matrix is an acellular pig dermal matrix, and the photosynthetic bacteria are purple non-sulfur photosynthetic bacteria. The invention provides application of the hydrogel in medical dressings for resisting bacteria and promoting wound healing. The bacterium-carrying hydrogel can efficiently sterilize, has excellent antibacterial performance and high water absorption rate, and can absorb tissue fluid exuded from a wound. Cell adhesion and tissue adhesion are not easy to occur, secondary infection and mechanical injury of skin wounds caused by dressing change are avoided, and healing of the wounds is facilitated. The hydrogel can be used for treating multidrug-resistant bacterial infection, realizing skin incision closure and improving the healing process.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a hydrogel loaded with photosynthetic bacteria and its preparation method and application. Background technique [0002] With the continuous emergence of drug-resistant strains, traditional antibiotics have been increasingly unable to meet the needs of clinical wound infection treatment. Since its discovery, microorganisms, especially Staphylococcus aureus MRSA, have been raging around the world. Once the skin wound is infected with MRSA, a severe inflammatory reaction will occur, damage the tissue, the wound is not easy to heal, and even cause death, which seriously threatens human health. . Most of the conventional clinical treatment methods are antibiotics, but this systemic medication has the side effects of causing superinfection and inducing drug resistance. In addition, the existing antibacterial agents such as various metal ions and nanoparticles are mostly exogenous...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0066A61L26/0057A61L2300/30A61L2300/404A61L2300/412
Inventor 李振华刘会芳赵尔曼张金超
Owner HEBEI UNIVERSITY
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