Method for simultaneously determining 20 free amino acids in cosmetics by dispersive solid-phase microextraction-amino acid automatic analyzer method

Abstract

The invention discloses a method for simultaneously determining 20 free amino acids in cosmetics by a dispersive solid-phase microextraction-amino acid automatic analyzer method. The method comprises the following steps of S01, preparing a standard solution; S02, pre-treating a sample; and S03, chromatographic conditions. According to the method for simultaneously determining the 20 free amino acids in the cosmetics by the dispersive solid-phase microextraction-amino acid automatic analyzer method, provided by the invention, a mobile phase gradient elution procedure is optimized, and extraction and purification conditions are investigated; the analysis method of the established automatic amino acid analyzer can be used for simultaneously and quantitatively determining 20 free amino acids (including 18 conventional amino acids and non-protein amino acids such as taurine and 5-aminolevulinic acid) in cosmetics; problems of many co-extracts, large matrix interference, quantitative errors caused by surfactant foam and the like in the cosmetic extraction process are solved, and an efficient, convenient, accurate and reliable analysis method is provided for cosmetic safety risk monitoring and quality control.

Description

technical field [0001] The invention relates to a method for simultaneously determining 20 kinds of free amino acids in cosmetics by a dispersed solid-phase microextraction-amino acid automatic analyzer method, belonging to the technical field of analysis and detection. Background technique [0002] There are a lot of free amino acids in the stratum corneum of human epidermal cells. These amino acids can completely penetrate the epidermal cells of the skin, give the skin vitality, improve the skin quality without affecting the skin barrier function, make the skin smooth, delicate and elastic, and play a role in moisturizing and maintaining skin stability. status plays an important role. Taurine and 5-aminolevulinic acid (hereinafter referred to as 5-ALA) are non-protein amino acids. Among them, taurine has the ability to revitalize skin cells, improve skin immunity, and resist external invasion of skin. 5-ALA widely exists in animal, plant and microbial cells. Its moisturi...

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Problems solved by technology

[0004] There are few reports on the detection methods of amino acids in cosmetics. At present, the line standard method of spectrophotometry after color development with ninhydrin is only used to determine the total amount of amino acids, and cannot determine the content of individual amino acids.

However, there are many types of amino acids, and one or more amino acids are usually added to cosmetic formulations. Therefore, the above industry standards have the limitation that they cannot effectively monitor the quality of raw materials and the stability of formulation processes, and are not suitable for the development of the industry and the daily safety risk monitoring of cosmetics.

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