Three-electrode photoelectrochemical chip and its application in the detection of glucose and mercury ions
A photoelectrochemical, three-electrode technology, applied in the direction of material electrochemical variables, can solve rare and other problems, and achieve the effect of simple use, simplified electrode modification process, and simple operation process
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Embodiment 1
[0092] A preparation method and detection of a photoelectrochemical cloth chip for enzyme-free glucose detection, comprising the following steps:
[0093] (1) Cloth chip obtained by screen printing technology
[0094] According to the method of Example 1 of Chinese invention patent ZL201510387790.8, a three-electrode cloth chip was prepared;
[0095] (2) Materials required for the modification of the working electrode (WE) of the synthetic cloth chip
[0096] First, polydiallyldimethylammonium chloride (PDDA) functionalized multi-walled carbon nanotubes (MWCNTs) (PDDA-MWCNTs) were prepared. Add 1g MWCNTs to 40mL H 2 SO 4 (95-98%) and HNO 3 (65-68%) in a mixed solution (v / v, 3:1), then ultrasonically shake the mixed solution for 8 hours at room temperature, and finally wash the solution to neutrality (pH 6.5-7.5) by repeated centrifugal washing, The obtained black solid was dried (80° C., 24 h) and stored at room temperature. 0.1 g of acidified MWCNTs was added into 20 mL...
Embodiment 2
[0105] Some important factors (PDDA-MWCNTs concentration, CdS QDs concentration, excitation wavelength, optical power density, voltage size, NaOH concentration) is optimized:
[0106] a) Preferred concentration of PDDA-MWCNTs
[0107] 1. The concentration of glucose to be tested is 100μΜ, the concentration of gradient PDDA-MWCNTs, the concentration of CdS QDs is 5.3g / L, the wavelength is 365nm, and the optical power density is 69.23mW cm -1 , the voltage is 0.1V, and the NaOH concentration is 0.1M.
[0108] 2. Several experimental groups were set up: the concentration of PDDA-MWCNTs was set to several different values (0g / L, 1g / L, 2g / L, 3g / L, 3.5g / L, 4g / L, 4.5g / L, 5g / L).
[0109] 3, steps and other materials are identical with embodiment 1, and test result is as follows figure 2 shown.
[0110] It can be seen from the experimental results that the photocurrent intensity increases with the increase of the concentration of PDDA-MWCNTs, and begins to decrease when the con...
Embodiment 3
[0137] The optimized conditions explored in Example 2 were used for quantitative detection of glucose on the photoelectrochemical cloth chip.
[0138] 1. Several experimental groups are set: glucose standard samples are set to several different values (0 μM, 0.05 μM, 0.1 μM, 1 μM, 10 μM, 50 μM, 100 μM, 500 μM, 1000 μM);
[0139] 2. Steps and other materials are the same as in Example 1, and the preferred parameters are the same as in Example 2.
[0140] Depend on Figure 8 It can be seen that there is a certain linear relationship between the photocurrent intensity (expressed by Y) and the logarithmic value of glucose concentration (expressed by X), the linear equation can be expressed as Y=4.033X+12.898, and the square value of the correlation coefficient is 0.9949.
[0141] The method used to calculate the detection limit is: X L =X b +3S b (X b is the average photocurrent signal of the blank control, S b is the standard deviation of the blank control) (five repeated...
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