Placenta protein 13 test strip and quantitative detection method

A quantitative detection method and placental protein technology, which are applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of unsuitable rapid and accurate quantitative detection, high background value, low sensitivity, etc., and achieve reliable diagnostic results and simple operation. , the effect of small size

Pending Publication Date: 2021-10-01
DONGGUAN DEYI BIOLOGICAL MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time, has low sensitivity, high background value, cumbersome operation, is prone to false positive and false negative results, and requires professionals to operate, which is not suitable for the clinical needs of ra

Method used

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  • Placenta protein 13 test strip and quantitative detection method
  • Placenta protein 13 test strip and quantitative detection method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example one

[0032] The amount of placental protein 13 of the present invention and the detection method of testing strips and detection methods include the following steps:

[0033] The test strip includes a PVC bottom plate 1, and a sample pad 2 is sequentially provided on the PVC floor 1, and the binding pad 3, the nitrocellulosic film 4, and the water absorbing pad are laminated on the bond pad 3, the The binding pad 3 and the water absorbing pad are disposed on both ends of the nitrocellulose film 4, respectively.

[0034] The binding pad 3 fixed a quantum point labeled anti-fed-free anti-fed-free antibiotic anti-bovine serum albumin and dinitrophenol coupling (rabbit anti-DNP-BSA);

[0035] The nitrocellulosic membrane 4 is provided with a detection line and a heat circuit, and the detection line fixes an antibody of another epitope of an anti-placental protein 13, and the quality wire is fixed to bovine serum albumin and dinitrophenol. Coupled (DNP-BSA).

[0036] Th...

Example Embodiment

[0039] Example 2

[0040] In the present embodiment, the tracer may also employ colloidal gold, fluorescent microspheres, gold magnetic nanos, up transfoliation, and time resolution fluorescence or the like. When different tracers are employed, the corresponding tracer detection instruments should be modified to quantify the optical intensity of the tracer. Among them, when the tracer is a colloidal gold, the instrument is a colloidal gold immunoassator. When the tracer is a fluorescent microsphere, the test instrument is a fluorescent immunoassator. When the tracer is a gold magnetic nano, the detection instrument is a gold magnetic nano-immunoassator. When the tracer is to forward the light material, the detection instrument is the upper turn-off immunoassator. When the tracer is time to resolve fluorescence, the detection instrument is a time-resolved fluorescent analyzer.

[0041] The remainder of the present embodiment is the same as that of the Embodiment, and the features o...

Example Embodiment

[0042] Example three

[0043] In the present embodiment, a quantum dot labeled sheep is fixed to the bonding pad 3, and rabbit IgG is fixed on the quality wire;

[0044] The remainder of the present embodiment is the same as that of the Embodiment, and the features of the embodiments are not explained in the present embodiment, and the interpretation of the first embodiment is not described herein.

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Abstract

The invention discloses a placenta protein 13 test strip and a quantitative detection method, and is characterized in that the test strip comprises a PVC base plate, the PVC base plate is sequentially provided with a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the sample pad is lapped on the combination pad, the combination pad and the water absorption pad are lapped at two ends of the nitrocellulose membrane respectively, an anti-placenta protein 13 antibody marked by a tracer and a quality control line ligand 1 marked by the tracer are fixed on the combination pad; and the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is fixedly provided with an antibody for resisting another epitope of the placenta protein 13, and the quality control line is fixedly provided with a ligand 2 which can be specifically combined with a ligand 1 of the quality control line.

Description

technical field [0001] The invention relates to the technical field of immunochromatographic quantitative detection, and in particular discloses a placental protein 13 test strip and a quantitative detection method. Background technique [0002] Placental protein 13 (placental protein 13, PP13) is one of the 56 members of the placental protein family discovered so far. It was extracted and isolated from human placenta tissue for the first time in 1983, and then through DNA sequence analysis and gene cloning and expression techniques, it was found that placental protein family 13 is a specific protein widely expressed in human placenta tissue. PP13 is a protein dimer with a molecular mass of 32ku. The primary structure of PP13 has a molecular mass of 16.5ku. It consists of two subunits with the same molecular mass. The two subunits are bound by disulfide bonds and bound to β-galactose The protein family has a high degree of homology. [0003] As pregnancy progresses, the le...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/558G01N33/68
CPCG01N33/543G01N33/558G01N33/68G01N2333/471
Inventor 罗进才叶伟滔
Owner DONGGUAN DEYI BIOLOGICAL MEDICAL TECH CO LTD
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