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Placenta protein 13 test strip and quantitative detection method

A quantitative detection method and placental protein technology, which are applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of unsuitable rapid and accurate quantitative detection, high background value, low sensitivity, etc., and achieve reliable diagnostic results and simple operation. , the effect of small size

Pending Publication Date: 2021-10-01
DONGGUAN DEYI BIOLOGICAL MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes a long time, has low sensitivity, high background value, cumbersome operation, is prone to false positive and false negative results, and requires professionals to operate, which is not suitable for the clinical needs of rapid and accurate quantitative detection of PP13
At present, there are few products for detecting PP13 in China, and there is an urgent need to establish a fast, convenient and accurate product or method for detecting PP13 in clinical practice.

Method used

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  • Placenta protein 13 test strip and quantitative detection method
  • Placenta protein 13 test strip and quantitative detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of placental protein 13 quantitative detection test strip and detection method of the present invention comprise the following steps:

[0033] The test strip comprises a PVC bottom plate 1, and the PVC bottom plate 1 is provided with a sample pad 2, a binding pad 3, a nitrocellulose membrane 4 and a water-absorbing pad 5 in sequence, and the sample pad 2 is lapped on the binding pad 3, and the The bonding pad 3 and the water-absorbing pad 5 are lapped on both ends of the nitrocellulose membrane 4 respectively.

[0034] Quantum dot-labeled anti-placental protein 13 antibody and quantum dot-labeled rabbit anti-bovine serum albumin and dinitrophenol conjugate (rabbit anti-DNP-BSA) are immobilized on the binding pad 3;

[0035] The nitrocellulose membrane 4 is provided with a detection line and a quality control line, the detection line is fixed with an antibody against another epitope of placental protein 13, and the quality control line is fixed with bovine serum a...

Embodiment 2

[0040] In this embodiment, colloidal gold, fluorescent microspheres, gold magnetic nanometers, upconversion luminescence, time-resolved fluorescence, etc. can also be used as tracers. When different tracers are used, the corresponding tracer detection instrument should be changed to an instrument capable of quantitatively detecting the optical intensity of the tracer. Wherein, when the tracer is colloidal gold, the detection instrument is a colloidal gold immunoassay analyzer. When the tracer is a fluorescent microsphere, the detection instrument is a fluorescent immunoassay analyzer. When the tracer is gold magnetic nanometer, the detection instrument is gold magnetic nanometer immune analyzer. When the tracer is an upconversion luminescent material, the detection instrument is an upconversion luminescence immunoassay analyzer. When the tracer is time-resolved fluorescence, the detection instrument is a time-resolved fluorescence analyzer.

[0041] The rest of this embodim...

Embodiment 3

[0043] In this embodiment, quantum dot-labeled goat anti-rabbit IgG is immobilized on the binding pad 3, and rabbit IgG is immobilized on the quality control line;

[0044] The rest of this embodiment is the same as that of Embodiment 1, and the features not explained in this embodiment are explained in Embodiment 1, and will not be repeated here.

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Abstract

The invention discloses a placenta protein 13 test strip and a quantitative detection method, and is characterized in that the test strip comprises a PVC base plate, the PVC base plate is sequentially provided with a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the sample pad is lapped on the combination pad, the combination pad and the water absorption pad are lapped at two ends of the nitrocellulose membrane respectively, an anti-placenta protein 13 antibody marked by a tracer and a quality control line ligand 1 marked by the tracer are fixed on the combination pad; and the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is fixedly provided with an antibody for resisting another epitope of the placenta protein 13, and the quality control line is fixedly provided with a ligand 2 which can be specifically combined with a ligand 1 of the quality control line.

Description

technical field [0001] The invention relates to the technical field of immunochromatographic quantitative detection, and in particular discloses a placental protein 13 test strip and a quantitative detection method. Background technique [0002] Placental protein 13 (placental protein 13, PP13) is one of the 56 members of the placental protein family discovered so far. It was extracted and isolated from human placenta tissue for the first time in 1983, and then through DNA sequence analysis and gene cloning and expression techniques, it was found that placental protein family 13 is a specific protein widely expressed in human placenta tissue. PP13 is a protein dimer with a molecular mass of 32ku. The primary structure of PP13 has a molecular mass of 16.5ku. It consists of two subunits with the same molecular mass. The two subunits are bound by disulfide bonds and bound to β-galactose The protein family has a high degree of homology. [0003] As pregnancy progresses, the le...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/558G01N33/68
CPCG01N33/543G01N33/558G01N33/68G01N2333/471
Inventor 罗进才叶伟滔
Owner DONGGUAN DEYI BIOLOGICAL MEDICAL TECH CO LTD
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