Homogalacturonan, compound, as well as preparation method and application

A technology of galacturonic acid oligosaccharides and uronic acid polysaccharides, which is applied in the field of polysaccharides, can solve the problems of no galacturonic acid polysaccharides, etc., and achieve the effect of improving anti-tumor immunity, eliminating sub-health status, and high yield

Active Publication Date: 2021-10-08
天津银杉科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No reports of galacturonic acid polysaccharides and their derivatives were found in the existing literature search

Method used

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  • Homogalacturonan, compound, as well as preparation method and application
  • Homogalacturonan, compound, as well as preparation method and application
  • Homogalacturonan, compound, as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Preparation of low polylactonic acid polysaccharides:

[0081] (1) Rinse, boiling, petroleum decolorization, petroleum ether degreasing 2 times, can provide by-products such as citrus essential oils, teolithins; ultrasonic pulverization, efficiently destroy the peel cell wall structure, and promote subsequent steps of crude skin polysaccharide release;

[0082] (2) Acidification pretreatment: dissolved with dilute hydrochloric acid or sulfuric acid, the pH is controlled at 5.0-6.0, 30 min, forms a stable water-soluble pectin gel, filtrate and slag;

[0083] (3) Enzymatic solution: add 6 mg / g of cellulase, maintain temperature 38 ° C, enzymatic hydrolysis time of 70 min;

[0084] (4) Alkali hydrolysis-microwave method: in a closed culture tank, aqueous solution of the gruge gel, add an isometric 4 mol / l NaOH, control pH at 12.0-13.0, microwave heating to 80-100 ° C, microwave energy can cause water Molecular resonance, the covalent bond interruption of long chain polysac...

Embodiment 2

[0094] Preparation of low polylactonic acid polysaccharide:

[0095] (1) Rinning apple peel;

[0096] (2) Alkali hydrolysis: The fruit skin is dissolved in 2L water, and 3 mol / l NaOH 400 ml was added, stirred for 30 min, cooled to room temperature;

[0097] (3) Enzymatic solution: add 5 mg / g of cellulase, maintain temperature 38 ° C, enzymatic hydrolysis time 90 min;

[0098] (4) Hg thick content does not reach 70%, no pre-protein step, use a wine yeast ( Saccharomycescerevisiae Fermentation method, extended fermentation degradation time: adding wine active dry yeast to a 2% sucrose solution, stir well, 2-4 hours in 25-30 ° C, activate it; in a water solution of 2-5% concentration, add 1-5% activated yeast, 30 ° C, fermented 24-48 hours;

[0099] (5) Fine extraction is performed using an ultrafiltration membrane, the ultrafiltration membrane is 1-10 nm, maintaining low pressure difference 0.05-0.5MPa continuously high flow extraction, and the molecular weight is 10 3 -10 4 ;

...

Embodiment 3

[0103] Low polysaccharide prepared using Example 1.

[0104] There is 625 parts by weight of oligoniduronic polysaccharide, 25 parts by weight of Astragalus, 10 parts by weight of the red dates and 8 parts by weight of the red dates.

[0105] Wash the Astragalus, fried hawthorn, red dates and tannin, ultrasonic smash, sieve, 10 times double steam soaked for 30 minutes, small fry 1 hour, filtrate, made into a dipper, then the dipper paste and oligonal lactose A hydrolycal polysaccharide is thoroughly mixed to make the powder.

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Abstract

The invention provides homogalacturonan, a compound as well as a preparation method and application. The invention provides a preparation method of homogalacturonan, which comprises the steps of 1, pretreating to obtain stable water-soluble pectin gel; 2, carrying out treatment by an alkaline hydrolysis-microwave method; 3, carrying out methoxyl removal treatment; and 4, refining with an ultrafiltration membrane. The invention also provides a compound applying the homogalacturonan as well as a preparation method and application. The compound disclosed by the invention has a good medical effect, and the preparation method is simple in process, high in yield and low in cost.

Description

Technical field [0001] The present invention relates to the field of polysaccharide, and more particularly to oligofaclonate polysaccharides, composites, and preparation methods and applications thereof. Background technique [0002] Homogalacturonan, HG) is extracted from the "modified citrus pectin, mcp) produced by apple, sunflower dishes, citrus, lemon skin, etc. After the glue polysaccharide is hydrolyzate, the molecular weight is smaller, the non-branched fiber, its chemical composition is uniform, easy to dissolve, and easy to absorb. [0003] Small molecules citrus pectin (LCP) is the second generation of products after the "modified pectin" of Santley, USA, is a mixture, chemical composition is unstable, and molecular weight span is 10kda- 20kda, esterification degree is 10% to 25%. Small molecule citrus pectin LCP is a mixture of various polysaccharides, containing rats, galacturonic glycan I (rhamngalacturonan I, RGI), Rat, galacturonic glycan II (Rhamngalacturonan II,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125A23L33/105A61K36/752A61P1/00A61P29/00A61P37/04A61P39/06A61K31/715
CPCC08B37/0045C08B37/0003A61K36/481A61K36/734A61K36/725A61K36/752A61K31/715A61P29/00A61P39/06A61P1/00A61P37/04A23L33/125A23L33/105A23V2002/00A61K2300/00A23V2250/51A23V2200/30A23V2200/308A23V2200/324A23V2200/32A23V2250/21
Inventor 沈彦伟俞露
Owner 天津银杉科技有限公司
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