Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof

A -D-1, glucan technology, applied in the field of paeoni linear α-D-1,4-glucan and its preparation, can solve the problems of easy to produce adverse reactions, difficult development and other problems, and achieve a small molecular weight , Reduce oxidative stress, facilitate absorption and utilization

Active Publication Date: 2021-10-08
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in addition to insulin injection, the clinical treatment of type II diabetes is mainly oral hypoglycemic drugs, some of which are chemically synthesiz

Method used

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  • Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof
  • Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof
  • Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] Example 1

[0044] The peony skin was dried, pulverized, the resulting powder was removed from 80% ethanol, and then 100 g of Danpi powder was added to 3000 ml of distilled water. After soaking at room temperature for 2 h, stirred and extracted, centrifuged, collect the supernatant, residue heating water After stirring, centrifugally, centrifuged, and then concentrated, then 5x volume anhydrous ethanol sink, precipitate freeze drying, and a polysaccharide with a polysaccharide and a polysaccharide yield of 5.6%;

[0045] Take a 1% solution to the crude polysaccharide to add an equal volume of dichloromethane / n-butanol mixed liquid (the volume ratio of dichloromethane and n-butanol is 4: 1), stirred by the protein, repeatedly deprotein 2 times The polysaccharide solution after protein concentrates, using a dialysis bag of 1000 Da, which is concentrated, and the dialysate is concentrated, and the polysaccharide is purified by Q-Sepharose Fast Flow anion exchange resin, and ...

Example Embodiment

[0053] Example 2

[0054] The peony skin was dried, pulverized, and the resulting powder was removed from 80% ethanol degreasing for 24 h. After drying at room temperature, 100 g of Danki powder was added to 2000 ml of distilled water, and after soaking at room temperature for 3 h, stirring extracting 2 h, centrifuged, collecting supernatant, residue heating water 2 times, centrifugal, centrifuged, and concentrated, combined with the supernatant, add 4x volume anhydrous ethanol sink, precipitate freeze drying, and a polysaccharide with a polysaccharide, a polysaccharide rate of 4.8%;

[0055] Take a 2% solution to the crude polysaccharide to add diploidal dichloromethane / n-butanol mixed liquid (6: 1 of dichloromethane and n-butanol having a volume ratio of 6: 1), stirring the protein, repeated off protein 2 Secondary, the polysaccharide solution after protein is concentrated, and the dialysis pocket is used in the dialysis bag of 1000 Da, and the dialysate is concentrated. , The...

Example Embodiment

[0057] Example 3

[0058] The peony skin was dried, pulverized, the resulting powder was removed from 80% ethanol, then dried at room temperature, adding 100 g of Danpi powder to add 2500 ml of distilled water, after soaking at room temperature for 4 h, stirring extraction of 2 h, centrifugally, collected supernatant, residue heating water 2 times, centrifugal, and concentrated, combined with the supernatant, add 4x volume anhydrous ethanol sink, precipitate freeze drying, and a polysaccharide, a polysaccharide yield of 4.5%;

[0059] Take a 5% solution of the crude polysaccharide to add triple volume of dichloromethane / n-butanol mixed liquid (the volume ratio of dichloromethane and n-butanol is 3: 1), stirred by the protein, repeatedly deprotein 2 Secondary, the polysaccharide solution after protein is concentrated, and the dialysis pocket is used in the dialysis bag of 1000 Da, and the dialysate is concentrated. , The water washed eluting component was collected, and the gel c...

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Abstract

The invention discloses a cortex moutan linear alpha-D-1,4-glucan as well as a preparation method and application thereof, and belongs to the technical field of polysaccharide extraction. The molecular weight range of the obtained natural linear alpha-D-1,4-glucan is 1.5-3.3 kDa. Cortex moutan is subjected to drying and smashing, heating reflux degreasing, room-temperature water extraction, centrifugation, supernate vacuum concentration, alcohol precipitation, vacuum suction filtration and freeze drying, and crude cortex moutan polysaccharide is obtained; and the crude cortex moutan polysaccharide is dissolved in water to form a crude cortex moutan polysaccharide solution, adding an organic solvent to remove protein, and conducting dialyzing, separating and purifying, concentrating and freeze-drying to obtain the cortex moutan linear alpha-D-1,4-glucan. The cortex moutan linear alpha-D-1,4-glucan has a protection effect on oxidative damage of pancreatic beta cells induced by high glucose, shows the effect of reducing blood sugar, and has no toxic or side effect on normal cells.

Description

technical field [0001] The invention relates to paeonol linear α-D-1,4-glucan and its preparation method and application, belonging to the technical field of polysaccharide extraction. Background technique [0002] Diabetes is one of the main chronic diseases that seriously endanger human health. Diabetes and its complications have become a worldwide public health problem that seriously endangers human health. It has attracted great attention from all over the world. Most diabetic patients belong to type 2 diabetes mellitus (T2DM) . Oxidative stress is the most fundamental cause of type II diabetes and its complications. Oxidative stress caused by the imbalance of oxidation and anti-oxidation can cause damage to pancreatic β cells, which is an important mechanism for the occurrence and development of diabetes. Under the conditions of high glucose environment, high fat state, cytokines and other conditions, the oxidative stress response of pancreatic β cells can be induced, ...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/716A61P3/10A23L33/125
CPCC08B37/0009C08B37/0003A61K31/716A61P3/10A23L33/125A23V2002/00A23V2200/328
Inventor 李红燕解万翠杨锡洪董秀芳车红霞宋琳
Owner QINGDAO UNIV OF SCI & TECH
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