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Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof

A -D-1, glucan technology, applied in the field of paeoni linear α-D-1,4-glucan and its preparation, can solve the problems of easy to produce adverse reactions, difficult development and other problems, and achieve a small molecular weight , Reduce oxidative stress, facilitate absorption and utilization

Active Publication Date: 2021-10-08
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in addition to insulin injection, the clinical treatment of type II diabetes is mainly oral hypoglycemic drugs, some of which are chemically synthesized hypoglycemic drugs, but they have the disadvantages of difficulty in development and easy to produce adverse reactions. Hypoglycemic active ingredients extracted from

Method used

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  • Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof
  • Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof
  • Cortex moutan linear alpha-D-1,4-glucan as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Dry and crush the Cortex Moutan, degrease the obtained powder with 80% ethanol for 24 hours, dry at room temperature, take 100g of Cortex Cortex Moutan and add 3000mL of distilled water, soak at room temperature for 2 hours, stir and extract for 2 hours, centrifuge, collect the supernatant, and heat the residue with water Stir and extract twice, centrifuge, combine the supernatants and concentrate, add 5 times the volume of absolute ethanol for ethanol precipitation, freeze-dry the precipitate, and obtain crude polysaccharides extracted from paeonol with hot water, and the yield of polysaccharides is 5.6%;

[0045] Take paeonol crude polysaccharide to make a 1% solution, add an equal volume of dichloromethane / n-butanol mixture (the volume ratio of dichloromethane and n-butanol is 4:1), stir to remove protein, repeat deproteinization 2 times , the polysaccharide solution after protein removal is concentrated, and dialyzed with a dialysis bag with a molecular weight cut-of...

Embodiment 2

[0054] Dry and crush the Cortex Moutan, degrease the obtained powder with 80% ethanol for 24 hours, dry at room temperature, take 100g of Cortex Cortex Moutan and add 2000mL of distilled water, soak at room temperature for 3 hours, stir and extract for 2 hours, centrifuge, collect the supernatant, and heat the residue with water Stir and extract twice, centrifuge, combine and concentrate the supernatant, add 4 times the volume of anhydrous ethanol for ethanol precipitation, and freeze-dry the precipitate to obtain the crude polysaccharide extracted from paeonol with hot water, and the polysaccharide yield is 4.8%;

[0055] Get the crude polysaccharide of paeonol and prepare it into a 2% solution, add double volume of dichloromethane / n-butanol mixture (the volume ratio of dichloromethane and n-butanol is 6:1), stir to remove protein, repeat deproteinization 2 Second, the polysaccharide solution after protein removal is concentrated and dialyzed with a dialysis bag with a molecul...

Embodiment 3

[0058] Dry and crush the Cortex Moutan, degrease the obtained powder with 80% ethanol for 24 hours, dry at room temperature, take 100g of Cortex Cortex Moutan and add 2500mL of distilled water, soak at room temperature for 4 hours, stir and extract for 2 hours, centrifuge, collect the supernatant, and heat the residue with water Stir and extract twice, centrifuge, combine and concentrate the supernatant, add 4 times the volume of anhydrous ethanol for ethanol precipitation, freeze-dry the precipitate, and obtain the crude polysaccharide extracted from paeonol with hot water, and the polysaccharide yield is 4.5%;

[0059] Get the paeonol crude polysaccharide and prepare it into a 5% solution, add three times the volume of dichloromethane / n-butanol mixed solution (the volume ratio of dichloromethane and n-butanol is 3:1), stir to remove protein, repeat deproteinization 2 Second, the polysaccharide solution after protein removal is concentrated and dialyzed with a dialysis bag wit...

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Abstract

The invention discloses a cortex moutan linear alpha-D-1,4-glucan as well as a preparation method and application thereof, and belongs to the technical field of polysaccharide extraction. The molecular weight range of the obtained natural linear alpha-D-1,4-glucan is 1.5-3.3 kDa. Cortex moutan is subjected to drying and smashing, heating reflux degreasing, room-temperature water extraction, centrifugation, supernate vacuum concentration, alcohol precipitation, vacuum suction filtration and freeze drying, and crude cortex moutan polysaccharide is obtained; and the crude cortex moutan polysaccharide is dissolved in water to form a crude cortex moutan polysaccharide solution, adding an organic solvent to remove protein, and conducting dialyzing, separating and purifying, concentrating and freeze-drying to obtain the cortex moutan linear alpha-D-1,4-glucan. The cortex moutan linear alpha-D-1,4-glucan has a protection effect on oxidative damage of pancreatic beta cells induced by high glucose, shows the effect of reducing blood sugar, and has no toxic or side effect on normal cells.

Description

technical field [0001] The invention relates to paeonol linear α-D-1,4-glucan and its preparation method and application, belonging to the technical field of polysaccharide extraction. Background technique [0002] Diabetes is one of the main chronic diseases that seriously endanger human health. Diabetes and its complications have become a worldwide public health problem that seriously endangers human health. It has attracted great attention from all over the world. Most diabetic patients belong to type 2 diabetes mellitus (T2DM) . Oxidative stress is the most fundamental cause of type II diabetes and its complications. Oxidative stress caused by the imbalance of oxidation and anti-oxidation can cause damage to pancreatic β cells, which is an important mechanism for the occurrence and development of diabetes. Under the conditions of high glucose environment, high fat state, cytokines and other conditions, the oxidative stress response of pancreatic β cells can be induced, ...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61K31/716A61P3/10A23L33/125
CPCC08B37/0009C08B37/0003A61K31/716A61P3/10A23L33/125A23V2002/00A23V2200/328
Inventor 李红燕解万翠杨锡洪董秀芳车红霞宋琳
Owner QINGDAO UNIV OF SCI & TECH
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