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A bioelectrochemical method for real-time in situ detection of BOD in organic wastewater

A technology of bioelectrochemistry and organic wastewater, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve the problems of increased BOD, decreased accuracy and stability of biosensors, etc., and achieve shortened test time and high test accuracy Effect

Active Publication Date: 2022-05-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some researchers have tried to reduce the reactor volume to shorten the time it takes for BOD to be completely consumed, the proportion of BOD consumed by planktonic microorganisms in this process has increased, resulting in a decrease in the accuracy and stability of the biosensor.

Method used

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  • A bioelectrochemical method for real-time in situ detection of BOD in organic wastewater
  • A bioelectrochemical method for real-time in situ detection of BOD in organic wastewater
  • A bioelectrochemical method for real-time in situ detection of BOD in organic wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Using the sludge water of the anoxic section of the sewage treatment plant as the inoculum, the bioelectrode for the detector was cultivated in the MFC mode:

[0069] An external resistor 18 is connected between the anode and the cathode of the bioelectrochemical reactor, and the loop is connected with copper wires to construct a microbial dye battery reactor (ie, an MFC reactor) 16, such as Pic 4-1 shown. The MFC reactor was inoculated with sludge water in the anoxic section. The inoculation process lasted for five days, and then it was transferred to the sequencing batch mode for cultivation, and the matrix solution was changed every day. The voltage of the MFC was recorded by a digital data collection instrument (KEYSIGHT model 34970A).

[0070] specific:

[0071] The reactor configuration with a cubic two-chamber structure was constructed into a bioelectrochemical reactor. The anode chamber 14 of the reactor had an inner diameter of 35 mm, a length of 40 mm and a...

Embodiment 2

[0077] The response current curve generated by the LSV (ie linear sweep voltammetry) scan of the bioelectrode in the solution containing the fixed organic components with different BOD concentrations (here, the sodium acetate solution is used as an example), the most suitable one is selected according to the curve data. The BOD detection process signal output potential.

[0078] After the electrode biofilm is matured, the external resistance of the MFC reactor is removed, and the bioelectrochemical reactor (including the mature bioelectrode and reference electrode placed in the anode compartment, and the counter electrode placed in the cathode compartment) are connected with the potentiostat. connected to construct a bioelectrochemical sensor 17, such as Figure 4-2 shown.

[0079] The bioelectrochemical reactor was connected with a potentiostat in a three-electrode system, graphite electrode (bioelectrode) 12, foamed nickel air electrode 13 and silver / silver chloride electro...

Embodiment 3

[0082] Taking the present invention as the detection method of the bioelectrochemical sensor in the fixed organic component BOD solution (taking sodium acetate solution as an example), the detection mark is measured:

[0083] The bioelectrochemical reactor was connected with a potentiostat in a three-electrode system, graphite electrode (bioelectrode), foamed nickel air electrode and silver / silver chloride electrode were used as working electrode, counter electrode and reference electrode, respectively. by adding 12.5mL -1 Trace Minerals and 5mL L -1 The standard BOD-containing solution was prepared by adding sodium acetate to 50mM PBS of vitamins, and the concentration of the standard BOD solution was set to 0.5mgBOD L respectively. -1 , 1mgBOD L -1 , 2mgBOD L -1 , 4mgBOD L -1 , 10mgBOD L -1 , 20mgBOD L -1 ,40mgBOD L -1 ,80mgBOD L -1 ,100mgBOD L -1 ,120mgBOD L -1 , 160mgBOD L -1 , 200mgBOD L -1 , 240mgBOD L -1 and 320mg BOD L -1 . The current at -0.1V potential...

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Abstract

The invention discloses a bioelectrochemical method for real-time and in-situ detection of BOD in organic wastewater, including 1): operating a bioelectrochemical reactor in a closed circuit in an MFC mode, and adding an inoculum solution to the anode chamber; 2): cultivating in a matrix solution Bioelectrode until the electrode biofilm matures; 3): The bioelectrochemical reactor is connected to a potentiostat to construct a bioelectrochemical sensor, and a linear voltammetry scan is performed, wherein the bioelectrode is placed in a single organic solution containing different BOD concentrations, Select the current at different potentials for fitting, determine the optimal signal output potential, and obtain the linear equation between the output current signal and the concentration of a single organic compound; 4): Obtain the linear equation between the output current signal and the concentration of each organic compound, and calculate the relative singleness of each organic compound Relative electricity production rate of organic matter; 5): Calculate the relative electricity production rate of organic wastewater, and the sensor outputs electrical signals in organic wastewater to obtain the BOD concentration of organic wastewater.

Description

technical field [0001] The invention belongs to the fields of biosensors and water pollutant detection, and relates to a pollutant detection technology based on bioelectrochemical reaction. The bioelectrochemical sensor can realize real-time in-situ BOD detection by using the detection method, and in particular relates to a real-time in-situ detection of sewage in sewage. Bioelectrochemical methods for BOD. Background technique [0002] BOD is the amount of dissolved oxygen required for the respiration and consumption of organic pollutants by aerobic microorganisms in wastewater, and is a key indicator for evaluating the level of organic pollution and biodegradability in wastewater. Organic pollutants are one of the common pollutants in organic wastewater, which cause hypoxia in the water body to damage the water body ecology, and can spread along the food chain, threatening human health. Existing organic pollution treatment technologies, such as electrochemical oxidation a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327G01N27/30
CPCG01N27/48G01N27/327G01N27/308
Inventor 成少安林朱凡
Owner ZHEJIANG UNIV
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