Method for evaluating safety of edible oil by using zebra fish

Abstract

The invention provides a method for evaluating the safety of edible oil by using zebra fish. The method comprises the following steps of constructing a zebra fish toxicity evaluation model, pre-treating the edible oil by extracting, degreasing and washing to obtain an extract of a detection product, treating via the zebra fish to obtain the toxicity evaluation data, and analyzing and evaluating the safety of the edible oil. The method provided by the invention is high in detection accuracy, scientific and reasonable in detection method and short in modeling period, so that the detection cost is greatly reduced, and practical feasibility is provided for popularizing and popularizing the detection method to the broad public, enterprises and governments.

Description

technical field [0001] The invention relates to the technical field of edible oil safety evaluation, in particular to a method for evaluating the safety of edible oil with zebrafish. Background technique [0002] Edible oil is the most common condiment used by residents, and it is also a medium for heating raw materials, which has the functions of seasoning and heat transfer. As one of the important nutrients needed by the human body, edible oil is an indispensable necessity in our daily life and plays an important role in the health of the human body. However, the current quality and safety problems have aroused the strong anger of consumers and the country's great attention. The quality and safety of edible oil mainly come from the various links of planting, harvesting, storage, processing and use of oil crops, specifically: [0003] (1) Aflatoxins (peanut oil, etc.), glucosinolates, oxazolidinethiones (rapeseed oil), gossypol (cottonseed oil), and benzopyrene (coconut...

AI Technical Summary

Problems solved by technology

[0006] (4) Heavy metal pollution

[0007] (5) The problem of benzopyrene, trans fatty acid and solvent in leaching crude oil brought about by processing exceeds the standard

[0009] (7) Oxidation and rancidity of oils and fats during oil storage, resulting in increased acid value and peroxide value

[0014] 1) Due to historical reasons, most of the detection indicators in the existing national standards are formulated with reference to foreign standards, but whether the toxicity of chemicals will show toxicity differences due to racial differences, there is currently no large sample data verification support, which means , there is uncertainty about whether a chemical that is harmless to Caucasians at standard doses is also harmless to Chinese people

[0015] 2) Unable to detect the synergistic toxicity of different chemical substances to the human body

Due to the unknown physical and chemical properties of different chemicals, there is uncertainty about whether they can produce synergistic effect or synergistic effect when interacting; it makes it more difficult to judge the synergistic toxicity of the sample to be tested than the toxicity of a single toxic substance

[0016] 3) Unknown additives cannot be detected, only known chemical substances can be detected

If there are analogues, derivatives of known chemical substances and their metabolites in the human body as unknown additives, it is difficult to detect

[0017] 4) There are many types of toxic substances, and considering cost factors such as time, manpower, and material resources, it is impossible to cover everything and achieve full inspection

[0020] After searching the literature, it is found that the safety evaluation of the zebrafish model is currently carried out, and the evaluation objects are all drugs and cosmetics, and there is no application in the field of edible oil.

Images