Pseudomonas nitroreductans, bacterial agent and their application and method for degrading acetaldehyde
A Pseudomonas, nitro technology, applied in the field of microorganisms, can solve the problems of acetaldehyde tolerance and harsh growth environment requirements, and achieve the effects of in-situ pollutant degradation, less interference, and easy operation.
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[0054] According to a specific embodiment of the present invention, the inorganic salt comprises sodium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium chloride, magnesium sulfate, calcium chloride and ferrous sulfate.
[0055] Wherein, the present invention does not specifically limit the amount of the inorganic salt added, which may be determined according to the type and content of the inorganic salt in the acetaldehyde-containing pollutant. For example, with NH 4 Cl 0.5-4g / L, NaCl4-8 g / L, KH 2 PO 4 0.2-0.8g / L, K 2 HPO 4 ·3H 2 O 0.2-0.8g / L, CaCl 2 0.01-0.05g / L, MgSO 4 Taking the mixed inorganic salt solution of 0.1-0.5 g / L as an example, based on the acetaldehyde-containing pollutants and / or the pollutants contained per kilogram, the addition amount of the inorganic salt mixed solution may be 1-3 mL / day.
[0056] In the present invention, when the acetaldehyde-containing pollutant is soil, in order to further promote the degradati...
preparation example
[0082] This preparation example was used to prepare the activated seed solution used in the following examples.
[0083] Take the LB medium, sterilize it at 121 °C for 20 min, and cool it to room temperature. The nitro-reducing Pseudomonas strain of the present invention was taken from a -80°C refrigerator, inoculated into the sterilized LB medium, and cultured at 170rpm and 30°C for 12 hours to obtain an activated seed solution.
Embodiment 1
[0085] This example is used to illustrate the effect of culture conditions on the ability of the strains of the present invention to degrade acetaldehyde.
[0086] The activated seed solution was inoculated into a carbon-free medium with an initial concentration of 500 mg / L of acetaldehyde according to different inoculum amounts (2%, 5%, 8%, 10%, 15%). Three parallels were set for each gradient, with blank medium as control. The shake flask was placed in a constant temperature shaker at a speed of 170 r / min and a temperature of 20 °C (considering the volatility of acetaldehyde, the temperature was controlled at 20 °C), and the samples were sampled at 0h, 6h, 12h and 24h. Aldehyde degradation rate and OD 600 .
[0087] The bacterial liquid cultured to the logarithmic phase was added to the medium with the initial concentration of 500 mg / L of sterilized and cooled formaldehyde at the optimal inoculum amount, and the shake flasks were placed in different pH values (5.0, 6.0, ...
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