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A kind of protein-free, DMSO-free cell cryopreservation medium, application and preparation method thereof

A cryopreservation solution and protein-free technology, applied in the field of cell culture, can solve the problems of cumbersome programmed freezing operation and unclear composition of cryopreservation solution, and achieve the effects of clear composition, long shelf life and wide application range

Active Publication Date: 2021-12-31
苏州依科赛生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a protein-free, DMSO-free cell cryopreservation solution, its application and its preparation method, aiming to solve the problem that the composition of the cryopreservation solution in the prior art is not clear, the addition of DMSO components has cytotoxicity, and the technology of cumbersome programmed freezing operation is required defect

Method used

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  • A kind of protein-free, DMSO-free cell cryopreservation medium, application and preparation method thereof
  • A kind of protein-free, DMSO-free cell cryopreservation medium, application and preparation method thereof
  • A kind of protein-free, DMSO-free cell cryopreservation medium, application and preparation method thereof

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Embodiment 1

[0035] A protein-free, DMSO-free cell freezing medium:

[0036] Including the following components: various amino acids, various vitamins, various inorganic salts, sucrose, propylene glycol, sodium carboxymethylcellulose.

[0037] Preferably, the components of the cell cryopreservation solution are: calcium chloride dihydrate 0.2g / L, potassium chloride 0.2g / L, sodium chloride 6.8g / L, anhydrous disodium hydrogen phosphate 0.1g / L , Sodium dihydrogen phosphate monohydrate 0.0543g / L, zinc sulfate heptahydrate 0.000432g / L, sodium bicarbonate 1.8g / L, D-glucose 2g / L, pyruvate 0.06g / L, DL-lipoic acid 0.00015g / L L, 1,4-butanediamine dihydrochloride 0.000081g / L, linoleic acid 0.000042g / L, L-alanine 0.034g / L, L-alanine-L-glutamine 0.54253g / L , L-arginine hydrochloride 0.1475g / L, L-asparagine 0.04g / L, L-aspartic acid 0.13g / L, L-asparagine methyl ester hydrochloride 0.075g / L, L-cystine hydrochloride 0.025g / L, L-cysteine ​​hydrochloride monohydrate 0.02g / L, L-glutamic acid 0.0147g / L, glyc...

Embodiment 2

[0055] A protein-free, DMSO-free cell freezing medium:

[0056] Including the following components: various amino acids, various vitamins, various inorganic salts, sucrose, propylene glycol, sodium carboxymethylcellulose.

[0057] Preferably, the components of the cell cryopreservation solution are: calcium chloride dihydrate 0.2g / L, potassium chloride 0.2g / L, sodium chloride 6.8g / L, anhydrous disodium hydrogen phosphate 0.1g / L , Sodium dihydrogen phosphate monohydrate 0.0543g / L, zinc sulfate heptahydrate 0.000432g / L, sodium bicarbonate 1.8g / L, D-glucose 2g / L, pyruvate 0.06g / L, DL-lipoic acid 0.00015g / L L, 1,4-butanediamine dihydrochloride 0.000081g / L, linoleic acid 0.000042g / L, L-alanine 0.034g / L, L-alanine-L-glutamine 0.54253g / L , L-arginine hydrochloride 0.1475g / L, L-asparagine 0.04g / L, L-aspartic acid 0.13g / L, L-asparagine methyl ester hydrochloride 0.075g / L, L-cystine hydrochloride 0.025g / L, L-cysteine ​​hydrochloride monohydrate 0.02g / L, L-glutamic acid 0.0147g / L, glyc...

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Abstract

The invention relates to a protein-free and DMSO-free cell freezing solution, its application and its preparation method. The cell freezing solution contains various amino acids, multiple vitamins, various inorganic salts, sugars, polyols, organic macromolecules molecular compound. The composition of the cell cryopreservation solution is simple and clear, and the cost is low, and high-safety macromolecular compounds, sugars, polyols and other components are used to protect the efficient storage of cells at low and ultra-low temperatures. The composition is clear, Stable and protein-free, it can avoid possible immune reactions in clinical applications and improve safety, and this product is generally suitable for cryopreservation of various types of cells. The present invention also provides a method for applying the cell cryopreservation solution. The cells preserved in the cryopreservation solution of the present invention can be revived after a short-term cryopreservation for one month, and the cell viability can be kept above 95%. The viability rate can still reach more than 90%, and the cell viability and function are not affected.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a protein-free and DMSO-free cell cryopreservation solution and its application. Background technique [0002] With the development of medical technology and the advancement of cell therapy methods, the requirements for in vitro cell culture are also getting higher and higher. The cell culture medium and cryopreservation solution in the prior art usually contain animal-derived substances with complex components. There are potential risks and increased chances of contamination. Therefore, the introduction of serum-free and protein-free cell culture media and cryopreservation solutions is extremely important for the research and development of medical research, and also provides the necessary technical basis for subsequent gene editing and individualized cell therapy. [0003] Cell cryopreservation, that is, by adding cryoprotectants to the solution, the cells are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 于宝利陈旭陈刚
Owner 苏州依科赛生物科技股份有限公司
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