No-load phase-inversion hydrogel microneedle, cobra sinensis neurotoxin phase-inversion hydrogel microneedle, and preparation method and application thereof
A Chinese cobra and neurotoxin technology, applied in the field of microneedles, can solve the problems of respiratory depression, side effects, pain and disgust of patients, etc., and achieve the effect of neat arrangement, vertical integrity of needles, and good mechanical properties.
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Embodiment 1
[0048] Example 1: Prescription screening of optimal needle excipients for phase inversion hydrogel microneedles of Chinese cobra neurotoxin
[0049] In the practical application of the microneedle transdermal drug delivery system, the patch part of the microneedle should be closely attached to the skin, which requires the patch to have a certain degree of flexibility, while the needle part of the hydrogel microneedle can only pierce the skin. The stratum corneum of the skin can release the drug in the skin to play a role, which also requires the microneedle to have sufficient mechanical strength, so it is necessary to screen the prescription for the auxiliary materials of the patch part and the needle body part.
[0050] The most important auxiliary material suitable for the preparation of phase inversion hydrogel microneedles is polyvinyl alcohol. Due to the limitations of practical applications, composite materials made of two or more polymer materials often have the properti...
Embodiment 2
[0082] Example 2: Preparation and performance verification of Chinese cobra neurotoxin phase inversion hydrogel microneedles
[0083] 2.1. Preparation of NANT-PTH-MNs
[0084] 2.1.1. Preparation of PVA solution
[0085] Refer to the method described in step 1.2.1 to prepare a 16% (w / w) PVA aqueous solution.
[0086] 2.1.2. Preparation of PVA / Dex solution
[0087] First absorb a certain amount of the above-prepared PVA solution, then add a certain amount of Dex powder into it, stir to make it evenly mixed, and make a mixed solution with a PVA / Dex ratio of 84 / 16 (w / w).
[0088] 2.1.3. Preparation of PVA / Dex needle drug-loading solution
[0089] When preparing the needle drug-loading solution, the different adding methods of the drug will cause the distribution of the drug in the mixed solution to be different. Experiments have found that adding Chinese cobra neurotoxin powder directly to the polyvinyl alcohol / dextran mixed solution, It will lead to uneven distribution of the...
Embodiment 3
[0147] Example 3: Study on the Analgesic Performance of Chinese Cobra Neurotoxin Phase-inversion Hydrogel Microneedles
[0148] Studies have shown that Chinese cobra neurotoxin has a good analgesic effect and has a good application prospect in the treatment of joint pain and cancer pain. Taking the NANT-PTH-MNs prepared in Example 2 as the test group, observe the pain threshold of the mice in each group after administration to thermal stimulation and the pain effect on the chemically stimulating acetic acid to explore the application of NANT-PTH-MNs. Whether MNs can produce good analgesic effect on mice will provide a reference for the clinical application of NANT-PTH-MNs.
[0149] 3.1. Animal materials
[0150] Kunming mice are half male and half female, weighing 18-22g, provided by the Experimental Animal Center of Anhui Medical University, certificate number: SCXK (Anhui) 2017-001. Within 12 hours before the experiment, no food or water was allowed.
[0151] 3.2. Experim...
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