System and article for prolonging generation of nitric oxide based on microencapsulated powder of spinach extract and acidic microencapsulated gel

A nitric oxide and microencapsulation technology, applied in the field of medicine, can solve problems such as high toxicity, and achieve the effects of simple operation, simple preparation process and high biological safety.

Active Publication Date: 2018-06-01
HEBEI JINGDING BIOMEDICINE TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the prior art (patent application CN201310355902.2, CN201310356220.3) has systems and methods for time-delayed production of nitric oxide using microencapsulated nitrite and acidified hydrogels, the NO donors used are nitrite Nitrates, and nitrites generally have certain toxicity, especially when the dose is large
In addition, the application of the delay system and operation method relies on one or several activation volumes of water, and there are certain limitations in specific operations

Method used

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  • System and article for prolonging generation of nitric oxide based on microencapsulated powder of spinach extract and acidic microencapsulated gel
  • System and article for prolonging generation of nitric oxide based on microencapsulated powder of spinach extract and acidic microencapsulated gel
  • System and article for prolonging generation of nitric oxide based on microencapsulated powder of spinach extract and acidic microencapsulated gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The preparation of embodiment 1 spinach extract

[0059] 1.1 Extraction of spinach

[0060] Wash the edible part of spinach with tap water and deionized water, dry it, chop it into small pieces, take 10g, put it into a large beaker, add 50mL of deionized water, adjust the pH of the extract to 3-4 with oxalic acid, grind it , put it in a 70°C water bath and incubate for 30min, filter the extract into a 100mL volumetric flask, dilute to 100mL with deionized water, adjust the pH of the extract to 3-4 with oxalic acid, then concentrate to about 10mL, transfer to a 250mL volumetric flask In a beaker, add 5mL saturated borax solution and 100mL (70-80°C) hot water, adjust the pH of the extract to 3-4 with oxalic acid, put it in a boiling water bath, heat for 15min, and shake it constantly, take it out, cool to room temperature, and then add 10mL of potassium ferrocyanide solution, 10mL of zinc acetate solution and 2g of activated carbon powder, after each addition, mix thorou...

Embodiment 2

[0065] The preparation of embodiment 2 microencapsulated spinach extract

[0066] The spinach extract prepared in Example 1 was concentrated, and a solution composed of spinach extract, hydrochloric acid-L-cysteine ​​and sodium metabisulfite (vitamin C stabilizer), zein and a volatile solvent was prepared by spray drying. Particles of zein-based spinach extract containing nitrite equivalent to 10% by weight. Zein is a proline-rich protein obtained from corn, which can be used as a substrate for coating and encapsulation in processed food and pharmaceuticals. It is listed as generally recognized as safe (GRAS) by the US Food and Drug Administration (FDA). The solution was 10% zein (Flo Chemicals, 29 Puffer St., Ashburnham, MA 01430 (Lot F40000111C6)) dispersed in a mixture consisting of ethanol:water (90:10). The solution also contains stabilizer hydrochloric acid-L-cysteine ​​and sodium pyrosulfite (vitamin C stabilizer), the content of hydrochloric acid-L-cysteine ​​is 0.1%...

Embodiment 3

[0067] Example 3 Release of Microencapsulated Spinach Extract and Acidic Microencapsulated Powder in Solution

[0068] A solution composed of citric acid, zein and a volatile solvent was prepared in the same manner as in Example 2 to prepare an acidic microencapsulated powder with zein as a matrix. It contains citric acid equivalent to 10% by weight.

[0069] Place 40ml (40ml) of water in a beaker, dissolve 10mg each of the above-prepared microencapsulated spinach extract and acidic microencapsulated powder in it, add it to the solution at zero time (0) at the specified time, and use amiNO The inNO-T nitric oxide measurement system (Innovative Instruments, Inc., Tampa, FL 33637) of the -700 probe detects the NO concentration in the solution and records the NO content in the solution. The production of NO is generated after adding particles, about At 20 minutes, the produced NO was formed from the liquid, and then NO gradually increased, reached a peak at about 1 hour, and the...

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Abstract

The invention relates to a nitric oxide releasing system with time-delay. The system comprises a microencapsulated spinach extract and acidic microencapsulated powder, and the acidic microencapsulatedpowder is provided with enough acidity for converting the spinach extract into nitric oxide. The spinach extract is used as a NO donor for continuously releasing NO on the body surface of a patient for a long time.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a microencapsulated medicament containing spinach extract as an active ingredient, a system and an article containing the microencapsulated medicament of the spinach extract. Background technique [0002] In mammals, NO is an endogenous physiological regulator in many physiological processes of the nervous system, immune system, and cardiovascular system. Its effects include relaxation of vascular smooth muscle, resulting in vasodilation of arteries and increased blood flow. NO is a neurotransmitter that has been implicated in neuronal activity and various functions ranging from avoidance learning to male and female genital erection (Kim et al., J. Nutrition 134(2004) 2873S). NO also partially regulates the cytotoxic effect of macrophages on microorganisms and tumor cells. In addition to mediating normal physiological functions, NO is also involved in different pathophysiolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K9/50A61K9/00A61P17/02A61P17/14A61P15/08
CPCA61K9/0014A61K9/0034A61K9/5052A61K9/5084A61K36/185
Inventor 陈振兴
Owner HEBEI JINGDING BIOMEDICINE TECH LTD
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