Micro-fluidic cell chip and virus isolated culture method based on same

Abstract

The invention discloses a micro-fluidic cell chip and a virus isolated culture method based on same. The micro-fluidic cell chip comprises a metal frame, a lower cover plate, a chip frame, a core chip and an upper cover plate, wherein a plurality of cell culture chambers are formed in the core chip; a main runner is formed in the surface of the core chip; and the main runner is communicated with the plurality of cell culture chambers. According to the virus isolated culture method based on the micro-fluidic cell chip, a method of co-culturing multiple cell lines is adopted, a to-be-analyzed sample and different cell lines are sequentially incubated, clinical samples can be obviously saved, and the sample utilization rate is improved to the greatest degree. Meanwhile, the consumption of a culture reagent can be reduced, and the separation and identification time is shortened. Because the micro-fluidic cell chip provided by the invention contains the plurality of built-in cell culture chambers, culture of at least ten different cell lines can be simultaneously realized theoretically, the screening flux of virus sensitive host cells is obviously increased, and high-flux, automatic and rapid screening of the virus sensitive cell lines is realized.

Description

technical field [0001] The invention belongs to the field of biological sciences, and in particular relates to a microfluidic cell chip and a virus isolation and culture method based on the cell chip. Background technique [0002] At present, new and re-emerged viral infectious diseases still pose a serious threat to human life and property. Therefore, the rapid identification of viruses is crucial to the early diagnosis of diseases and the prevention and control of epidemics. As we all know, the virus plaque test based on living cell culture is the gold standard for virus diagnosis and follows the "Koch's law". When a new viral infectious disease occurs, an important issue is to identify the sensitive host cell line allowed by the virus by plaque test, which is especially critical for subsequent virus amplification, virus isolation and culture, and vaccine production. [0003] Existing conventional virus isolation and culture methods are usually carried out in polystyrene...

AI Technical Summary

Problems solved by technology

This traditional culture method usually requires a large number of cells (10 5 -10 8 1), the use of medium and other reagents is large, the experimental period is long (7-10 days), and the sensitivity is low; for major viral infectious diseases, the clinical samples are often more precious, when the viral load in the sample is low, The possibility of failure of isolation and culture is extremely high; if the isolated and cultured virus lacks an in vitro culture system (such as human norovirus), it is impossible to develop an effective antiviral strategy

At present, when the virus is isolated and identified through cell culture and infection, it is mainly to inoculate different cell lines in separate cell culture flasks or in the same microplate and then infect them separately. The operation steps are cumbersome and prone to cross-contamination. Realize rapid and high-throughput screening of virus-sensitive host cells and rapid isolation and culture of viruses

[0004] In actual work, it is difficult to obtain infected samples of major viral infectious diseases. Even if the virus-containing material is obtained, the viral load is very low after treatment, and it is almost impossible to isolate and culture by conventional methods. Virus taxonomy, identification, and vaccine development pose major obstacles and waste precious virus samples

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