The invention relates to the field of agricultural mechanical equipment, specifically to an agricultural weeding machine capable of preventing weed regeneration. The agricultural weeding machine comprises an outer shell, a bottom plate, a weeding mechanism, a conveying mechanism, a rolling mechanism, a soil loosening mechanism, a first rotating motor, a weeding rotating roller, a first fixed rod,cams, weeding part mounting cavities, rotating mounting rods, weeding parts, first driving connecting rods, a second rotating motor, a third rotating motor, rolling rollers, crushing knives, rolling balls, a soil loosening roller, a plurality of soil loosening rods, a second fixed rod, connecting rods, connecting supports, soil-loosening rotating shafts and soil-loosening crushing knives. The agricultural weeding machine provided by the invention is capable of completely removing the root parts of weeds, destroying the seed structure of the weeds and preventing weed regeneration in the weedingprocess; and the agricultural weeding machine is incapable of being damaged when encountering harder soil in the soil loosening process.