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Rare earth up-conversion energy transfer nano sensing platform, construction method and application

A rare earth up-conversion and energy transfer technology, applied in the field of spectroscopy applications, can solve the problems of complex FRET construction process, uneven morphology or size, unstable detection signal, etc., achieving excellent light absorption performance, excellent fluorescence quenching performance, Uniform size effect

Pending Publication Date: 2021-11-12
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these nanoreceptors usually or partially have problems such as complex preparation, uneven shape or size, and colloidal instability, which lead to complex FRET construction process and unstable detection signals.

Method used

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  • Rare earth up-conversion energy transfer nano sensing platform, construction method and application
  • Rare earth up-conversion energy transfer nano sensing platform, construction method and application
  • Rare earth up-conversion energy transfer nano sensing platform, construction method and application

Examples

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preparation example Construction

[0068] In some embodiments, in step S2, the preparation method of the polydopamine nanoparticles comprises the following steps:

[0069] adding dopamine into a mixed solvent of ethanol and water, and vigorously stirring to obtain a third reaction liquid;

[0070] Add concentrated ammonia water (volume is 0.2-2% of the above mixed solvent) to the third reaction liquid, continue to stir and react at room temperature for 24 hours to obtain the fourth reaction liquid;

[0071] Centrifuging the fourth reaction solution to collect the bottom sediment;

[0072] After the precipitate is washed with pure water, it is ultrasonically dispersed into an aqueous solution to obtain the polydopamine nanoparticles.

[0073] Further, the mass ratio of dopamine, ethanol and water is 0.01:3:1-0.1:3:1.

[0074] In summary, the preparation process of the polydopamine nanoparticles used in the present invention is quite simple, the size is uniform and adjustable, the surface has abundant functiona...

Embodiment 1

[0082] Example 1 Construction of UCPs-PDA-based upconversion fluorescence resonance energy transfer system and its detection and analysis of antibiotics (such as: chloramphenicol)

[0083] (1) Preparation and water-soluble modification of rare earth up-conversion luminescent materials

[0084] With 1mmol Ln(CF 3 COO) 3 The aqueous solution of (Y:Yb:Er=78:20:2) is the precursor, octadecene and oleic acid are the solvents, heated to 150°C under magnetic stirring to form oleic acid rare earth salt, cooled to room temperature, and then added to the system Add methanol (contains: NH 4 F and NaOH), stirred for 0.5h, then heated to 100°C to remove methanol, then raised the temperature of the system to 290°C under argon atmosphere, reacted for 1.5h, cooled to room temperature, added ethanol to precipitate out, and washed with ethanol for 3 Second, the precipitate is oil-soluble UCPs. Subsequently, weigh 300mg of polyacrylic acid (PAA), 30ml of diethylene glycol, and pass nitrogen ...

Embodiment 2

[0091] Example 2: Construction of UCPs-PDA-based upconversion fluorescence resonance energy transfer system and its detection and analysis of food-borne toxins (such as: aflatoxin b1, AFB1)

[0092] (1) Preparation and water-soluble modification of rare earth up-conversion luminescent materials

[0093] With 1mmol Ln(CF 3 COO) 3 The aqueous solution of (Y:Yb:Er=78:20:2) is the precursor, octadecene and oleic acid are the solvents, heated to 150°C under magnetic stirring to form oleic acid rare earth salt, cooled to room temperature, and then added to the system Add methanol (contains: NH 4 F and NaOH), stirred for 0.5h, then heated to 100°C to remove methanol, then raised the temperature of the system to 290°C under argon atmosphere, reacted for 1.5h, cooled to room temperature, added ethanol to precipitate out, and washed with ethanol for 3 Second, the precipitate is oil-soluble UCPs. Subsequently, weigh 300mg of polyacrylic acid (PAA), 30ml of diethylene glycol, and pass...

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Abstract

The invention discloses a rare earth up-conversion energy transfer nano sensing platform, a construction method and application. The rare earth up-conversion energy transfer nano sensing platform comprises: an amino-modified nucleic acid aptamer; a fluorescent donor material, wherein one end of the nucleic acid aptamer is covalently modified on the surface of the fluorescent donor material; and a fluorescent donor material modified with the nucleic acid aptamer is assembled on the surface of the fluorescent acceptor material through [pi]-[pi] interaction, wherein the fluorescence donor material is a rare earth up-conversion luminescent material, and the fluorescence acceptor material is polydopamine. The fluorescent receptor polydopamine adopted by the invention has the advantages of simple preparation, uniform size, good water solubility, excellent fluorescence quenching performance and the like, can effectively quench up-conversion luminescence, and has higher quenching efficiency, so that the detection sensitivity is improved; and the constructed rare earth up-conversion energy transfer nano sensing platform has higher signal-to-noise ratio and sensitivity on target detection.

Description

technical field [0001] The invention relates to a rare earth up-conversion energy transfer nano-sensing platform and its construction method, as well as its application in target detection, and belongs to the field of spectrum application technology. Background technique [0002] Fluorescence resonance energy transfer (FRET) is a highly sensitive, high spatial resolution, non-destructive homogeneous detection technology that combines fluorescence spectroscopy with resonance energy transfer technology. FRET means that the fluorescence donor and acceptor are in the range of 1-10nm. When the emission spectrum of the donor overlaps with the absorption spectrum of the acceptor, due to the dipole-dipole interaction, the excited state energy of the donor is in the form of non-radiation delivered to the recipient. This technology has been used in the field of analytical chemistry for more than 40 years, and it has greatly promoted the development of biomedical analysis while contin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/02C09K11/85
CPCG01N21/6428C09K11/7773C09K11/025G01N2021/6432
Inventor 王宇辉郑建萍邓晨
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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