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Small-molecule fluorescent probe for aluminum ion detection and cell imaging, and application thereof

A cell imaging and fluorescent probe technology, applied in the field of detection, can solve the problems of high toxicity, small toxic and side effects, and large molecular weight, and achieve the effects of small toxic and side effects, rapid colorimetric judgment, and small detection limit.

Active Publication Date: 2021-11-19
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the aluminum ion probes in the prior art, which mainly contain large conjugated groups or Schiff base organic compounds (containing more than 3 benzene rings) through complex synthesis, and have the disadvantages of large molecular weight and high toxicity, the present invention provides A small molecule fluorescent probe and its application for aluminum ion detection and cell imaging are disclosed. The fluorescent probe is a simple conventional small molecule (1 benzene ring, molecular weight less than 198), and has little toxic and side effects, and a wide range of raw materials

Method used

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  • Small-molecule fluorescent probe for aluminum ion detection and cell imaging, and application thereof
  • Small-molecule fluorescent probe for aluminum ion detection and cell imaging, and application thereof
  • Small-molecule fluorescent probe for aluminum ion detection and cell imaging, and application thereof

Examples

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Embodiment 1

[0069] A small molecule fluorescent probe for aluminum ion detection and cell imaging, the small molecule fluorescent probe is 2,5-dihydroxyterephthalic acid (abbreviated as DB), and its molecular formula is: C 8 h 6 o 6 , the structural formula is:

[0070]

[0071] The described small molecule fluorescent probe for aluminum ion detection and cell imaging, under ultraviolet light irradiation, DB emits yellow fluorescence, adding Al 3+ After the fluorescence is shown in green, and the Al 3+ The higher the concentration, the more obvious the green fluorescence;

[0072] The described small molecule fluorescent probe for aluminum ion detection and cell imaging, DB in the fluorescence spectrum has Al 3+ When , the fluorescence intensity is significantly enhanced, accompanied by a blue shift phenomenon, and with the Al 3+ As the concentration increases, the fluorescence intensity increases continuously;

[0073] The described small molecule fluorescent probe for aluminum ...

Embodiment 2

[0076] A test paper detection method for small molecule fluorescent probes for aluminum ion detection and cell imaging, comprising the following steps:

[0077] 1) The qualitative filter paper is cut into strips of pH test paper size;

[0078] 2) Put the filter paper cut in the previous step into 2×10 -4 Soak in mol / L DB aqueous solution to ensure that the filter paper is attached with DB probe compound for testing aluminum ions;

[0079] 3) Take out the filter paper soaked or sprayed in the previous step with tweezers, and place it in the air to dry naturally;

[0080] 4) Set 1.0×10 -8 mol / L-1.0×10 -3 The solution to be tested with different aluminum ion concentrations in mol / L was sprayed onto the DB test paper obtained in the previous step, and placed under 365nm ultraviolet light to observe the color change, and the color changed from yellow to bright green.

Embodiment 3

[0082] A test paper detection method for small molecule fluorescent probes for aluminum ion detection and cell imaging, comprising the following steps:

[0083] 1) Cut the quantitative filter paper into strips of pH test paper size;

[0084] 2) Spray 2×10 directly on the filter paper cut in the previous step -4 mol / L DB aqueous solution until wetted, to ensure that the DB probe compound is attached to the filter paper to detect aluminum ions;

[0085] 3) Take out the soaked or sprayed filter paper in the previous step with tweezers, place it in a vacuum drying oven for 30-60 minutes at a temperature of 45°C, and make DB test paper after drying;

[0086] 4) Set 1.0×10 -8 mol / L-1.0×10 -3 The solution to be tested with different aluminum ion concentrations in mol / L was dropped onto the DB test paper obtained in the previous step, and placed under 365nm ultraviolet light to observe the color change, and the color changed from yellow to bright green.

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Abstract

The invention discloses a small-molecule fluorescent probe for aluminum ion detection and cell imaging, and application thereof. The fluorescent probe is a simple and conventional small molecule (1 benzene ring, the molecular weight is less than 198), the toxic and side effects are small, and the raw material source is wide. When the aluminum ions are detected, only simple ultraviolet irradiation is needed, interference of coexisting ions is avoided, rapid colorimetric judgment can be achieved, and the content of the aluminum ions can be more accurately quantified through a fluorescence spectrum. The existence of the aluminum ions can be rapidly detected on the test paper by combining the fluorescent probe molecule with ultraviolet irradiation, a solution to be detected can be prepared into a solution to be quantitatively detected by using fluorescence spectrum, and the detection limit is relatively small.

Description

【Technical field】 [0001] The invention relates to the technical field of detection, in particular to a small molecule fluorescent probe for aluminum ion detection and cell imaging and applications thereof. 【Background technique】 [0002] Due to its excellent chemical and physical properties, metal aluminum is widely used in various fields of production and life, such as water treatment plants, food additives, drugs, antiperspirants, deodorants, aluminum cookware, cans, bleached flour, anti-acid agent, light alloy production, etc. Due to the impact of acid rain and human activities, free Al in the environment 3+ The exposure of aluminum is increasing day by day. Although aluminum is a non-essential element for living systems, Al 3+ size (0.051nm) and charge, making it a Mg 2+ (0.066nm), Ca 2+ (0.099nm) and Fe 3+ (0.055nm), Zn (0.060nm) and other competitive inhibitors of several essential elements with similar characteristics, resulting in inability to scavenge Al due to...

Claims

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Application Information

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IPC IPC(8): C07C65/03C09K11/06G01N21/64
CPCC07C65/03C09K11/06G01N21/6428G01N21/643G01N21/6486C09K2211/1007
Inventor 吕红颖蒋炳丽张啸峰陈飞遐周川童罗小辞吴杰
Owner GUILIN MEDICAL UNIVERSITY
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