Mixed gel for injection and preparation method and application thereof

A mixed gel and injection technology, applied in the field of medical and cosmetic materials, can solve the problems of prolonged hyaluronic acid retention time, fast polylactic acid hydrolysis rate, collapse or adhesion of microspheres, etc., and achieve good biocompatibility of degradation products. , The filling effect is good, the effect of maintaining mechanical properties

Active Publication Date: 2021-12-03
BEIJING DATSING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the retention time of hyaluronic acid obtained by this method is significantly prolonged in the body, the preparation method is too cumbersome and is not conducive to popularization and application.
Some researchers proposed to add polylactic acid microspheres to hyaluronic acid gel, but the hydrolysis rate of polylactic acid is fast and unstable, and the microspheres may collapse or stick after mixing with hydrogel, and the validity period in vivo is short. Moreover, the degradation product of polylactic acid is lactic acid, which is acidic and prone to inflammation and granuloma formation.

Method used

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  • Mixed gel for injection and preparation method and application thereof

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Effect test

Embodiment 1

[0035] This embodiment provides a hyaluronic acid mixed gel containing P34HB microspheres for injection, the preparation method of which is as follows:

[0036] Dissolve 5 g of P34HB with a molecular weight of 100,000 in the organic solvent dichloromethane, dissolve at 60°C for 30 minutes, add it to a 1% polyvinyl alcohol (PVA) aqueous solution, stir at a constant speed for 4 hours, heat to remove the organic reagent, and add a large amount of purified water to wash PVA was removed three times, the filter cake was collected by filtration, freeze-dried at low temperature for 24 hours, and P34HB microspheres of 30-50 microns were sieved.

[0037] Dissolve 0.4g of hyaluronic acid in 2ml of water for injection, add 0.2ml of 20% sodium hydroxide solution, add 10 microliters of 1,4-butanediol diglycidyl ether, and add 1M hydrochloric acid after 24 hours of cross-linking to adjust The osmotic pressure is adjusted by pH and PBS swelling, and a gel with a sodium hyaluronate mass fracti...

Embodiment 2

[0040] This embodiment provides a hyaluronic acid mixed gel containing P34HB-PEG microspheres for injection, the preparation method of which is as follows:

[0041] Dissolve 5 g of copolymer P34HB-PEG with a molecular weight of 100,000 (molar ratio is 4:1) in dichloromethane, an organic solvent, and then add it to a 1% aqueous solution of polyvinyl alcohol (PVA), stir at a constant speed for 4 hours, and remove by heating For organic reagents, a large amount of purified water was added to wash three times to remove PVA, the filter cake was collected by filtration, freeze-dried at low temperature for 24 hours, and 30-50 micron P34HB-PEG microspheres were screened out.

[0042] Dissolve 0.4g of hyaluronic acid in 2ml of water for injection, add 0.2ml of 20% sodium hydroxide solution, add 10 microliters of 1,4-butanediol diglycidyl ether, and add 1M hydrochloric acid after 24 hours of cross-linking to adjust The osmotic pressure is adjusted by pH and PBS swelling, and a gel with a ...

Embodiment 3

[0045] This embodiment provides a hyaluronic acid mixed gel containing P34HB-collagen microspheres for injection, the preparation method of which is as follows:

[0046] 5 g of copolymer P34HB-collagen (molar ratio 4:1) with a molecular weight of 120,000 was dissolved in the organic solvent methylene chloride, and then added to a 1% polyvinyl alcohol (PVA) aqueous solution, stirred at a constant speed for 4 h, and heated Organic reagents were removed, a large amount of purified water was added to wash three times to remove PVA, the filter cake was collected by filtration, freeze-dried at low temperature for 24 hours, and 30-50 micron P34HB-collagen microspheres were screened out.

[0047] Dissolve 0.4g of hyaluronic acid in 2ml of water for injection, add 0.2ml of 20% sodium hydroxide solution, add 10 microliters of 1,4-butanediol diglycidyl ether, and add 1M hydrochloric acid after 24 hours of cross-linking to adjust The osmotic pressure is adjusted by pH and PBS swelling, an...

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Abstract

The invention provides mixed gel for injection and a preparation method and application thereof. The mixed gel for injection is prepared from a gel matrix and polyhydroxyalkanoate microspheres, the molecular weight of the polyhydroxyalkanoate microspheres is 50000-500000 Dalton, the average particle size of the polyhydroxyalkanoate microspheres is 30-50 microns, and the mass fraction of the polyhydroxyalkanoate microspheres in the mixed gel is 15%-30%. The mixed gel formed by mixing the PHA microspheres with specific molecular weight and average particle size with the gel matrix is good in filling effect, can maintain mechanical properties in the degradation process, and can stimulate collagen hyperplasia, activate skin, effectively and progressively smooth wrinkles and realize skin contour remodeling after being injected into the dermis layer of the skin, so that the effect of filling for a long time is achieved; and in addition, the degradation product 3-hydroxybutyric acid is a natural energy source of human cells, and can promote fiber cell growth and collagen regeneration, so that the beautifying and filling effects are more obvious.

Description

technical field [0001] The invention relates to the technical field of medical aesthetic materials, in particular to a mixed gel for injection and its preparation method and application. Background technique [0002] In recent years, with the rapid development of medical and aesthetic technology, injectable gels can achieve minimally invasive treatment or filling beauty at the injection site, which has received extensive attention. [0003] At present, hyaluronic acid (HA) and its salts are the main gels for injection on the market. Hyaluronic acid is an acidic mucopolysaccharide macromolecular substance widely present in connective tissues of humans and animals. Glue is injected into the folds and depressions of the dermis or the desired plumpness in the form of fillers, which can achieve the effect of removing wrinkles and modifying the face. Now it has the potential to replace collagen, placenta and botox. However, hyaluronic acid is easy to diffuse in the body and be de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/50A61L27/52A61L27/20A61L27/24
CPCA61L27/18A61L27/50A61L27/52A61L27/20A61L27/24A61L2400/06C08L67/04C08L5/08C08L71/02C08L77/04
Inventor 李晓盟牛睿李俊昊周天泓刘紫薇翟晖代金明
Owner BEIJING DATSING BIO TECH
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