Traditional Chinese medicine compound composition for treating hypertension and quality detection method thereof

A composition and high blood pressure technology, applied in the direction of drug combination, medical formula, measuring device, etc., can solve the problems of unclear mechanism, inability to effectively relieve dizziness and headache, and low quality of clinical research, achieving good repeatability and stability, The effect of treating spontaneous hypertension with high precision

Active Publication Date: 2021-12-10
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although a variety of treatment measures have been adopted clinically, such as systemic and comprehensive treatments such as improving lifestyle, lowering blood pressure, lowering lipids, and surgical operations, they still cannot effectively relieve symptoms such as dizziness and headache in some hypertensive patients and delay the delay in heart, brain, kidney and other target organs. damage process
Hypertension is one of the dominant diseases of traditional Chinese medicine, but the quality of clinical research is not high, and the mechanism is still unclear

Method used

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  • Traditional Chinese medicine compound composition for treating hypertension and quality detection method thereof
  • Traditional Chinese medicine compound composition for treating hypertension and quality detection method thereof
  • Traditional Chinese medicine compound composition for treating hypertension and quality detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] The preparation method of Pinggan Yishen Granules specifically comprises the following steps:

[0094] (1) Take 15g Gastrodia elata, 15g Uncaria vine, 15g Shayuanzi, 15g Tribulus terrestris, 10g Chuanxiong, 10g Achyranthes bidentata, 12g Eucommia ulmoides, 12g mistletoe, 10g Jiaoshanzhi, add water to decoct and extract twice, add up for the first time Water 6 times the weight of medicinal materials, first soak for 0.5h, then decoct to extract for 0.5h, filter; add 6 times the amount of water for the second time, decoct and extract for 0.5h, filter. The filtrate was concentrated under reduced pressure to a relative density of about 1.20 (60°C), centrifuged in a straight-tube high-speed centrifuge (speed: 16000r / min, flow rate: 4-6L / min), and the supernatant after centrifugation was taken.

[0095] (2) Take an appropriate amount of dextrin, put it in a fluidized bed, set the inlet air temperature to 100°C, and when the temperature of the material rises to 70°C, start to f...

Embodiment 2

[0096] Example 2 TLC identification of Pinggan Yishen Granules, Pinggan Yishen Granules are prepared from Example 1, the specific steps are as follows:

[0097] Step 1: To identify Uncaria TLC, the specific steps are as follows:

[0098] 1) Take 15g of the test sample of Pinggan Yishen Granules, dissolve it to 40mL, adjust the pH to 10 with concentrated ammonia water, extract with chloroform, twice in total, 30mL each time, combine the chloroform extract, steam After drying, add 1 mL of methanol to the residue to dissolve it, and use it as the test solution for Pinggan Yishen. Get Uncaria japonicus preparation, prepare the negative test solution of Uncaria uncaria in the same way;

[0099] 2) Take another 2 g of Uncaria reference drug powder, add 2 mL of concentrated ammonia water, soak for 30 minutes, add 50 mL of chloroform, heat and reflux for 2 hours, let cool, filter, take 10 mL of filtrate, evaporate to dryness, add 1 mL of methanol to dissolve the residue, and use as ...

Embodiment 3

[0136] The content determination method of embodiment 3 Pinggan Yishen Granules

[0137] (1) Chromatographic conditions: chromatographic column: HederaC18ODS-2 chromatographic column (4.6mm×250mm, 5μm); mobile phase: methanol: acetonitrile (1:1) as phase A, 0.1% phosphoric acid as item B, carry out gradient according to Table 1 Elution; column temperature: 35°C; flow rate: 1.0mL min -1 . Detection wavelength: 280nm; injection volume: 10μL. The number of theoretical plates is not less than 3000 based on the gastrodin peak;

[0138] Table 1 Gradient elution table

[0139] time (min) Mobile phase A(%) Mobile phase B(%) 0～5 7→13 93→87 5～15 13→20 87→80 15～23 20→30 80→70 23～28 30→37 70→63 28～35 37→65 63→35

[0140] (2) Preparation of reference substance solution: Accurately weigh gastrodin, geniposide, ferulic acid and sagirin reference substance, add 70% methanol to dissolve, sonicate for 10min, constant volume, shake well, and ma...

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Abstract

The invention discloses a traditional Chinese medicine compound composition for treating hypertension and a quality detection method thereof. The traditional Chinese medicine compound composition consists of gastrodia elata, uncaria rhynchophylla, semen astragali complanati, tribulus terrestris, ligusticum wallichii, radices cyathulae, eucommia ulmoides, mistletoe and fried gardenia jasminoides. According to the traditional Chinese medicine compound composition for treating the hypertension and the quality detection method thereof, the optimal raw material composition and dosage part ratio are screened out through a large number of experiments according to characteristics of spontaneous hypertension and principles of syndrome differentiation and treatment of traditional Chinese medicine, and a prepared traditional Chinese medicine compound can obviously reduce systolic pressure and has a good effect of treating the spontaneous hypertension. According to the traditional Chinese medicine compound composition for treating the hypertension and the quality detection method thereof, a detection method is screened through the large number of experiments, thin-layer chromatography qualitative identification and high performance liquid chromatography are adopted for quantitative detection, the content of uncaria rhynchophylla, gastrodia elata, ligusticum wallichii and radices cyathulae in a formula can be comprehensively and objectively detected and analyzed, and methodological detection shows that the high performance liquid chromatography is high in precision, and good in repeatability and stability.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a compound traditional Chinese medicine composition for treating hypertension and a quality detection method thereof. Background technique [0002] Spontaneous hypertension is caused by a combination of genetic and environmental factors. It is a progressive cardiovascular syndrome of many etiologies that results in changes in the function and structure of the heart and blood vessels. Therefore, the primary goal of essential hypertension treatment is to minimize the overall risk of cardiovascular death and disability. Among the nearly 1,000 daily outpatients in the Cardiology Department of our hospital, hypertensive patients account for about 30%. Although a variety of treatment measures have been adopted clinically, such as systemic and comprehensive treatments such as improving lifestyle, lowering blood pressure, lowering lipids, and surgical operations, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8988A61K9/16G01N30/02G01N30/90A61P9/12
CPCA61K36/8988A61K36/74A61K36/481A61K36/185A61K36/236A61K36/21A61K36/46A61K36/744A61K9/1652A61P9/12G01N30/02G01N30/90A61K2236/331A61K2300/00Y02A50/30
Inventor 方祝元吴磊王琼戴国梁张思奇
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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