Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polyene macrolide compound as well as preparation method and application thereof

A technology of macrolides and compounds, which is applied in the field of polyene macrolides and their preparation, can solve problems such as food safety and environmental pollution, the rapid development of traditional agriculture, and achieve broad development and application prospects and good control effects Effect

Active Publication Date: 2021-12-10
HUNAN INST OF MICROBIOLOGY
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A series of food safety and environmental pollution problems caused by chemical pesticide residues have become one of the bottlenecks restricting the rapid development of traditional agriculture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polyene macrolide compound as well as preparation method and application thereof
  • Polyene macrolide compound as well as preparation method and application thereof
  • Polyene macrolide compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Take the strain preservation solution of the Actinospora mutant strain, inoculate it on the ISP2 medium plate, and cultivate it for 15 days at a temperature of 28°C, and take an appropriate amount of Actinospora spores from the plate to inoculate Cultivate in the fermentation medium for 20 days at a temperature of 28°C to obtain a fermentation broth;

[0076] (2) Mix the fermented liquid obtained in step (1) with an equal volume of methanol and extract for 15 hours, repeat the extraction for 3 times, mix the extraction solutions obtained by the extraction for 3 times, and carry out vacuum distillation and concentration to obtain the extract , take 40g of the extract and fully dissolve it with methanol, then centrifuge to obtain a supernatant, and filter the supernatant to obtain an extract solution;

[0077] (3) The extract solution that step (2) obtains is loaded on the 100-200 mesh normal-phase silica gel column, and successively with the cyclohexane of 300-500mL,...

Embodiment 2

[0093] Example 2 The activity test and MIC value determination of active ingredient C to human pathogenic fungi

[0094] a. The antibacterial zone size of common human pathogenic fungi by the antibacterial component B containing active ingredient C in Example 1 was measured by agar diffusion method.

[0095] The tested strains were Candida albicans, Cryptococcus neoformans, and Saccharomyces.cerevisiae, respectively. Use the inoculation loop to pick a little bacterial liquid from the glycerol storage tube and streak on the plate to cultivate the activated strain overnight, and pick a single colony to inoculate Obtain a culture solution with good growth status in an appropriate amount of PDB medium, then spread it on the PDA medium, and after drying, punch a hole in the center of the plate, and add about 80 μL of the antibacterial component containing active ingredient C in Example 1 B, cultivate overnight, measure the diameter of the inhibition zone in the morning of the next ...

Embodiment 3

[0098] Example 3 Active ingredient C is tested for the activity of phytopathogenic fungi and the determination of MIC value

[0099] a. The diameter of the bacteriostatic zone of the antibacterial component B containing the active ingredient C in Example 1 against filamentous phytopathogenic fungi was detected by the agar diffusion method or the confrontation culture method.

[0100] When the tested strain is Fusarium graminearum, the pathogen of wheat scab, Fusarium graminearum, the confrontation culture method is adopted: culture Fusarium graminearum, the pathogen of wheat scab, on a PDA plate for 2-3 days, and then use a hole puncher on the outer edge of mycelial growth. Punch some holes, take out the mycelia block with an inoculation needle, place it in the center of a new PDA plate, inoculate the Actinospora mutant strain in Example 1 with an inoculation loop at a distance of 3 cm from the center, and in a temperature of 28° C. Cultivate under conditions for 3-5 days, obs...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fungus control technology, and discloses a polyene macrolide compound as well as a preparation method and application thereof. The structural formula of the polyene macrolide compound provided by the invention is shown as a formula (I). The preparation method of the polyene macrolide compound provided by the invention comprises the following steps: performing fermentation culture on actinosporium to obtain fermentation liquor, and separating and purifying the fermentation liquor to obtain the polyene macrolide compound. The invention also provides an application of the polyene macrolide compound to prevention and treatment of plant fungal diseases and an application to preparation of antifungal drugs. The polyene macrolide compound not only has a good prevention and treatment effect on plant fungal diseases, but also has an obvious inhibition effect on animal superficial and systemic fungal infection.

Description

technical field [0001] The invention relates to fungal control technology, in particular to a polyene macrolide compound and its preparation method and application. Background technique [0002] Soil-borne diseases caused by plant pathogenic fungi affect the quality and quantity of agricultural products, and will cause significant economic losses if not properly controlled. At present, chemical pesticides are still the main force in crop disease management. However, chemical pesticides not only pollute agricultural products, affect product quality, damage the ecological environment, and threaten human health, but also cause pests to develop resistance and reduce the control effect after long-term use. In recent years, with the continuous improvement of people's living standards, the demand for "organic food" and "pollution-free vegetables" continues to increase. A series of food safety and environmental pollution problems caused by chemical pesticide residues have become on...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H17/04C07H1/06A01N43/90A01P3/00
CPCC07H17/04C07H1/06A01N43/90Y02A50/30
Inventor 唐滢刘清术郭照辉雷平张翠央杜杰龙青山欧阳薇
Owner HUNAN INST OF MICROBIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com