Acidomyces acidothermus and application thereof in leaching copper-containing pollutants of waste circuit board
A pollutant and leaching technology, applied in the directions of water pollutants, microorganism-based methods, bacteria, etc., can solve the problems of environmental hazards, low production costs, affecting the operating environment, etc. simple effect
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Embodiment 1
[0042] Embodiment 1: the screening of bacterial strain
[0043] (1) The density obtained from Changzhou Sewage Treatment Plant is 0.027g·mL -1 Put 10mL of the sludge into 90mL LB liquid medium, and cultivate it at 140-180rpm, 30-35℃ for 4-6 days;
[0044] (2) Take the bacterial solution in step (1) and inoculate it into a new 100mL LB liquid medium with an inoculation volume of 5-10% by volume, and cultivate it in an environment of 140-180rpm and 30-35°C for 4-6 days;
[0045] (3) Take the bacterial solution in step (2) and inoculate it into a new 100mL LB liquid medium with an inoculation amount of 5-10% by volume, and cultivate it in an environment of 140-180rpm and 30-35°C for 4-6 days;
[0046] (4) Take 5 μL of the bacterial solution in step (3) and add it to the sterilized LB liquid medium, culture it in a shaker at 140-180 rpm and 30-40°C for 4-6 days, and then suck 100 μL with a pipette tip Liquid, spread on acidic LB solid medium;
[0047](5) Put the acidic LB solid...
Embodiment 2
[0050] Embodiment 2: the identification of bacterial strain
[0051] (1) Get the bacterial solution obtained in Example 1, and observe the morphological characteristics of the bacterial strain using an optical microscope. The result is as figure 1 As shown, the strain thallus is fluffy, the colony is round, and the colony is opaque.
[0052] (2) The strains were sent to Shanghai Sangon Bioengineering Co., Ltd. for strain identification using the 16SrDNA method.
[0053] After using ITS and NS universal primers for amplification, the primers and amplification system are as follows: the amplified bands were detected by electrophoresis. Electropherogram as figure 2 Shown in middle lane 3 (the band size is around 950bp). The bands were sequenced, and the sequencing results were compared on BLAST. After comparison, the similarity between the amplified sequence and Acidomyces was 100%.
[0054] ① Primers used:
[0055] ITS1: TCCGTAGGTGAACCTGCGG (SEQ ID NO: 1),
[0056] ITS4:...
Embodiment 3
[0063] Embodiment 3: the application of Acidomyces acidothermus bacteria in leaching copper
[0064] Effect of Adding Different Amounts of Bacteria Solution on Copper Leaching Effect
[0065] 1. The effect of 20mL bacterial solution on the leaching effect
[0066] (1) Inoculate the above-screened bacterial strains into acidic LB liquid medium, and cultivate them at 140-180rpm, 30-35°C until the bacterial liquid OD 600 The value is 0.8;
[0067] (2) Take 20mL of the bacterial solution obtained in step (1), add 1g of waste circuit board powder to it, stir at a speed of 180r / min on a magnetic stirrer, draw a sample every 4h, and measure the copper in the sample ion concentration;
[0068] (3) The sample liquid is centrifuged, and the supernatant after taking the high-speed low-temperature centrifuge is filtered to obtain the required clear and transparent leachate;
[0069] (4) After diluting the leaching solution in step (3) 250 times, utilize ICP to measure when adding 20mL...
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