Method for rapidly preparing mineralized photosensitizer nano-drug and application of mineralized photosensitizer nano-drug
A nano-drug and photosensitizer technology, applied in the field of biomedical materials, can solve problems such as slow metabolism, low safety, and slow clearance, and achieve the effects of simple preparation method, inhibitory activity, and good biocompatibility
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Embodiment 1
[0041] (1) Configure the reaction system with different final concentrations of manganese ions. The configuration method is as follows: take 50 μL of β-(4-carboxyphenoxy)zinc phthalocyanine solution with a concentration of 89.2 μM and add it to 1930 μL DMEM cell culture medium, and add A certain amount of 1M manganese chloride aqueous solution (2-20μL), add water to supplement the volume of the system to 2mL, so that the final concentrations of manganese ions added are 1, 2, 4, 6, 8, 10mM, β-(4 carboxyphenoxy Base) The final concentration of zinc phthalocyanine was 2.2 μM.
[0042] (2) Put the above solution into a constant temperature incubator for mineralization, the reaction temperature is 37° C., and after 30 minutes of reaction time, blue turbid mineralization appears in the solution.
[0043] (3) Centrifuge the above mineralization solution at a speed of 5000-12000rpm for 5min to obtain a blue precipitate, wash the precipitate with deionized water 2-3 times, then wash wi...
Embodiment 2
[0046] (1) Configure manganese ion and calcium ion mineralization reaction systems in PBS: take 50 μL of β-(4carboxyphenoxy)zinc phthalocyanine solution with a concentration of 89.2 μM in each group and add it to 1850 μL PBS, and the manganese ion group Add 100 μL of 1M manganese chloride aqueous solution; add 100 μL of 1M calcium chloride aqueous solution to the calcium ion group, and the total volume of each group is 2000 μL; the final concentration of manganese in the manganese ion group is 50 mM, and the final concentration of calcium in the calcium ion group is 50 mM. In the group, the final concentration of zinc β-(4carboxyphenoxy)phthalocyanine was 2.2 μM.
[0047] (2) The above solutions were put into a constant temperature incubator for mineralization, the reaction temperature was 37° C., and after 60 minutes of reaction time, blue turbid mineralized substances appeared in the solutions respectively.
[0048] (3) Centrifuge the above mineralization solution at a speed...
Embodiment 3
[0051] (1) Configure reaction systems with different final concentrations of manganese ions. The configuration method is as follows: take 200 μL of Prussian blue solution with a concentration of 116.4 μM and add it to 1760 μL DEMEM cell culture medium, and add a certain amount of 1M manganese chloride aqueous solution (2-40 μL) to each group. , add water to supplement the volume of the system to 2 mL, so that the final concentrations of manganese ions added are 1, 2, 4, 6, 8, 10, 20 mM respectively, and the final concentration of Prussian blue is 11.6 μM.
[0052] (2) Put the above solution into a constant temperature incubator for mineralization, the reaction temperature is 37° C., and after 15 minutes of reaction time, blue turbid mineralization appears in the solution.
[0053] (3) Centrifuge the above mineralization solution at a speed of 5000-12000rpm for 5min to obtain a blue precipitate, wash the precipitate with deionized water 2-3 times, then wash with absolute ethanol...
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