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Biological mask based on amniotic basement membrane and preparation method

A technology of biological facial mask and amniotic membrane basement membrane, applied in the field of biomedical materials, can solve the problems of skin damage, single, chemical composition facial mask stimulation, etc., and achieve the effects of repairing tissue and scars, and simple preparation process

Pending Publication Date: 2021-12-28
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, it is necessary to provide a biological mask based on amnion basement membrane that solves the existing chemical component mask to stimulate and damage the skin and has a single effect for the defects in the prior art.

Method used

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  • Biological mask based on amniotic basement membrane and preparation method

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preparation example Construction

[0036]In addition, the present application also provides a preparation method of the amniotic basement membrane-based biological mask, which includes the following steps: cross-linking the mask carrier with the surface of the epithelial cell layer of the amnion basement membrane through a chemical condensation reaction.

[0037] In some of the embodiments, the facial mask carrier is made of natural polymer material or amniotic membrane without sponge layer, fibroblast layer and compact layer.

[0038] In some of the embodiments, the natural polymer material includes one or more of collagen, elastin, hyaluronic acid, gelatin, fibrinogen, and hydrogel.

[0039] In some of these embodiments, the amniotic basement membrane is the amniotic membrane. After removing the spongy layer, fibroblast layer and dense layer and retaining the epithelial cell layer and basement membrane, the amniotic membrane is crushed, lysed, separated and acid-base balanced. and made.

[0040] Specifically...

Embodiment

[0048] Example - in vitro cell experiment test Cytotoxicity experiment of biomembrane based on amnion basement membrane The mouse fibroblasts in the logarithmic growth phase were inoculated in a 96-well culture plate, and each hole was inoculated with 1.5 / mL×10 4 / mL cell suspension 200 μL, cultured for 24 hours. The cytotoxicity test was carried out with the MTT cell proliferation and cytotoxicity detection kit: the experiment was divided into an experimental group and a control group. The experimental group was a multifunctional biological amnion mask prepared by the above preparation method, and the positive control group was a common chemical Component facial mask, the experimental group and the control group were operated according to the steps of the kit, and finally the absorbance at 570nm was measured with a microplate reader. The relative cell proliferation percentage (RGR) was calculated, and the level of cytotoxicity was assessed. The results are shown in Table 1: ...

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Abstract

He invention provides a biological mask based on amniotic basement membrane. The biological mask based on the amniotic base membrane comprises the amniotic base membrane and a mask carrier, the mask carrier is cross-linked with the surface of an epithelial cell layer of the amniotic base membrane through a chemical condensation reaction, and the amniotic base membrane retains the base membrane and the epithelial cell layer of the amniotic base membrane, various secreted bioactive components and different glue elements can achieve anti-inflammation, tissue and scar repairing, nutrition, breathability, calcification resistance and no immunogenicity, and the technical problems that an existing chemical component mask stimulates and damages skin, and a biological mask is single in effect are solved. In addition, the invention further provides a preparation method of the biological mask based on the amniotic basement membrane.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a biological mask based on amnion basement membrane and a preparation method thereof. Background technique [0002] As a common beauty product, facial mask is widely loved by consumers because of its convenient use and obvious effect. In recent years, its development has shown a rapid growth trend. Its main focus is: (1) Develop biological facial masks to reduce chemical components Masks, get more audiences. The advantages of biological facial masks are self-evident. They have good hydrophilicity and oxygen permeability, and have a good fit with the skin, without irritation or damage to the skin. (2) Develop natural, green and non-irritating mask materials and products. Bio-mask has the function similar to the skin, no irritating substance and odor, not easy to grow bacteria, does not contain fluorescent substances, easy to clean, and is a pure natural ...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/02A61K8/65A61K8/73A61Q19/00
CPCA61K8/982A61K8/0212A61K8/65A61K8/735A61Q19/00A61K2800/805
Inventor 周辉王月虹
Owner GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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