Preparation method of pH-responsive zanthoxylum seed antibacterial peptide microcapsule
A technology of Zanthoxylum bungeanum and antimicrobial peptides, which is applied in the field of preparation of pH-responsive Zanthoxylum bungeanum antimicrobial peptide microcapsules, can solve problems such as the influence of antimicrobial peptide activity, and achieve the effects of prolonging antibacterial time, low cost, and extending food shelf life
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Embodiment 1
[0029] To prepare a prickly ash seed protein antibacterial peptide, the specific steps are as follows:
[0030] Select the Zanthoxylum bungeanum protein (content: 245.70mg / g) obtained by alkali treatment, accurately weigh 5g of Zanthoxylum bungeanum protein and dissolve it in a sufficient amount of ethanol solution, preheat it in a water bath, and adjust the enzymatic hydrolysis temperature to 32°C for liquefaction For enzymatic hydrolysis, use HCl to adjust the pH value of the solution to 2, add pepsin for enzymatic hydrolysis, and continuously add HCl and NaOH during the enzymatic hydrolysis process to maintain the pH value of the solution at 2, after enzymatic hydrolysis for 3 hours, put it in a 90°C water bath to extinguish Enzyme, cooling, pour the cooled mixture into a beaker, place in an ultrasonic cleaner with cooling water circulation, ultrasonically extract antibacterial peptides, ultrasonic power is 150W ultrasonic 5min, after ultrasonication, centrifuge at 8000r / min...
Embodiment 2
[0032] Take 4 groups (three parts for each group) of prickly ash seed protein (content: 245.70mg / g, 5g for each part) and dissolve them in a sufficient amount of solvent, preheat in a water bath, adjust the enzymolysis temperature to 32°C for liquefaction and enzymolysis, At the same time, use HCl to adjust the pH value of the solution to 2, add pepsin for enzymolysis, and continuously add HCl and NaOH to maintain the pH value of the solution during the enzymolysis process. Then centrifuge at 8000r / min for 10min, take the supernatant, and vacuum freeze-dry. (Wherein group ac also adopts ultrasonic-assisted extraction after cooling the enzyme, the mixture after cooling is about to be poured into the beaker, placed in the ultrasonic cleaner of cooling water circulation, ultrasonic extraction antimicrobial peptide, ultrasonic power is 150W ultrasonic 5min), statistics each Group productivity, specific preparation process and productivity are shown in Table 1.
[0033] Table 1
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Embodiment 3
[0036] The Zanthoxylum bungeanum protein antibacterial peptide obtained in Example 1 is divided into 4 groups, and the concentration is 1.0wt% shellac resin sodium salt mixes evenly respectively, then inhales in the syringe, uses the high-voltage electrostatic spray capsule forming device, sprays the mixed solution to Mass concentration of 1% CaCl 2 The solution was allowed to stand and solidify for 30-45 minutes. After the microcapsule suspension was centrifuged at high speed, it was fully washed with deionized water, and then vacuum freeze-dried for 24 hours to obtain 4 groups of Zanthoxylum bungeanum antimicrobial peptide microcapsules. Select pork with a uniform appearance, divide it into small pieces of about 10g, select 10g of fresh pork and put it directly into a food-grade polyethylene (PE) plastic plate and cover it with plastic wrap as a control group, and then mix 4 groups of pepper seed antimicrobial peptide microcapsules evenly Sprayed on the surface of fresh pork...
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