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A kind of bifidobacteria that can relieve colitis and its application

A bifidobacterium, ulcerative colitis technology, applied in the direction of bifidobacteria, application, bacteria used in food preparation, etc., can solve the problems of no ulcerative colitis prevention and treatment, to protect the intestinal barrier function, Inhibiting inflammation and reducing the effect of disease activity index

Active Publication Date: 2022-08-09
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no effective measures for the prevention and treatment of ulcerative colitis. Existing therapeutic drugs such as aminosalicylic acid, glucocorticoids, and immunosuppressants all have certain side effects. Therefore, research on new alternative treatments method is very important

Method used

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  • A kind of bifidobacteria that can relieve colitis and its application
  • A kind of bifidobacteria that can relieve colitis and its application
  • A kind of bifidobacteria that can relieve colitis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089]Example 1: Screening, identification, culture, observation and preservation of Bifidobacterium pseudocatenulatum N2

[0090] 1. Filter

[0091] Take 1 g of healthy infant fecal samples from Harbin, apply gradient dilution to MRS solid medium (including rapid screening), and place them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; pick the surface wet , The raised, white and yellowed colonies were streaked on the MRS solid medium, purified and cultured under anaerobic conditions at 37 °C, and this operation was repeated 3 times to obtain a purified single colony; pick a single colony MRS solid medium was streaked and cultured anaerobic at 37°C for 36h.

[0092] 2. Identification

[0093] Extract the genome of the strain obtained by screening, amplify and sequence the 16S rDNA of the strain (the nucleotide sequence of the amplified 16S rDNA is shown in SEQ ID NO. 1), and carry out the obtained sequence in NCBI-Blast The nucl...

Embodiment 2

[0099] Example 2: Preparation of Bifidobacterium pseudocatenulatum N2 bacterial suspension (1) Dip the bacterial liquid of Bifidobacterium pseudocatenulatum N2 from a glycerol tube and spread on MRS solid medium Line, cultured at 37°C for 48h in an anaerobic environment to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, cultured at 37°C for 48h in an anaerobic environment for activation and culture, repeat this operation 3 times to obtain the activated bacteria liquid. (2) The activated bacterial liquid obtained in step (1) is inoculated into the MRS liquid medium according to the inoculum amount of 2% (v / v), cultured at 37° C. for 24 hours to obtain a fermentation liquid, and the fermentation liquid is centrifuged to collect bacteria The cells were resuspended in physiological saline, and the number of viable cells was adjusted to 5×10 9 CFU / mL, made into bacterial suspension.

Embodiment 3

[0100] Example 3: Tolerance of Bifidobacterium pseudocatenulatum N2 to the digestive tract: the strain was adjusted to 1.0×10 9 CFU / mL, placed in gastric juice and incubated for 2h, then transferred to intestinal juice and incubated for 2h. The survival rate of the experimental strains was calculated as follows:

[0101]

[0102] Note: N1 is the surviving strain after treatment, and N0 is the total untreated strain.

[0103] Bifidobacterium pseudocatenulatum (Bifidobacterium pseudocatenulatum) N2 had a higher survival rate in the digestive tract and had no significant difference with BB-12. This result shows that Bifidobacterium pseudocatenulatum N2 can survive in the digestive tract.

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PUM

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Abstract

The present invention disclosed a rapid screening method and application of bifidobacterium alleviated alleviated colon, which belongs to the field of biotechnology.Fake small chain bisidobacteria storage is located at the Chinese typical cultivation center, Wuhan, the preservation date is July 5, 2021, the preservation number is: CCTCC M2021820.Fake small chain bisidobacterium can significantly improve the weight loss, colon length and disease activity index during the DSS induction of colitis, enhance the integrity of intestinal barrier, inhibit tissue damage, marrow peroxidase (MPO) activity, PGE2 level level levelAnd to promote inflammation factor.Compared with the model group mice, the fake small chain dualobacterium KLDS N2 significantly lowered the expression of DSS stimulating mice to promote mice, the expression, and the expression of sticky protein and closely connected protein mRNA.

Description

[0001] Technical Field: The present invention relates to a strain of Bifidobacterium dentis that can relieve colitis and its application, and belongs to the field of biotechnology. technical background: [0002] Ulcerative colitis is a chronic idiopathic intestinal inflammatory disease. The early clinical manifestations are blood-type diarrhea. With the continuation of the disease, symptoms such as diarrhea, blood in the stool, weight loss, and vomiting may occur. In addition, complications such as toxic colonic dilatation, intestinal perforation, polyps, and enteritis may be caused. The incidence of ulcerative colitis in my country is on the rise. [0003] Ulcerative colitis is mainly characterized by inflammation of the colorectal mucosa and submucosa. Under normal circumstances, the body is jointly protected by a physical barrier composed of intestinal epithelial cells and surface mucus, a chemical barrier composed of immune-mediated antibacterial substances, and a microbi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/04A61P1/00A23L33/135A23L2/52C12R1/01
CPCC12N1/20A61K35/745A61P1/04A61P1/00A23L33/135A23L2/52A23V2002/00A23V2400/535A23V2200/3204Y02A50/30
Inventor 李柏良赵莉梁胜男董佳欢霍贵成刘飞王松
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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