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Trichoderma koningiopsis, root soaking liquid and application

A technology of trichoderma pseudocorning and soaking roots, which is applied in the direction of application, chemicals for biological control, biocides, etc., can solve the problems of unsuitable ginseng growth, pesticide residues, low soil bulk density, etc., and achieve the protection of soil microbial populations structure, enhance the biological abundance, and the effect of high seedling survival rate

Active Publication Date: 2022-01-11
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the problems of low soil bulk density, easy compaction, and low nutrient content in ordinary farmland, ginseng directly planted with ginseng has serious diseases, resulting in low yield and poor quality of ginseng
Moreover, some plots still have the problem of pesticide residues, which are not suitable for the growth of ginseng

Method used

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  • Trichoderma koningiopsis, root soaking liquid and application
  • Trichoderma koningiopsis, root soaking liquid and application
  • Trichoderma koningiopsis, root soaking liquid and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] This example provides screening, isolation and identification methods for Trichoderma koningiopsis Tri112 strain.

[0067] The Trichoderma pseudokoningensis provided by the invention is isolated from the ginseng rhizosphere soil of the ginseng planting base in Zuojia Town, Jilin City, Jilin Province.

[0068] The rhizosphere soil adopts the dilution coating plate method. Take 10g of ginseng rhizosphere soil and add it to a conical flask filled with 90mL of sterile water, shake it on a shaker at 150r / min for 30min, draw 1mL of uniformly mixed soil from the conical flask and suspend it Liquid, serially diluted with sterile water, respectively from 10 -3 、10 -4 、10 -5 Take 200 μL of the three gradients and add it to the PDA medium plate to spread evenly. Place the plate at 25°C for 3 days, pick the mycelium of a single Trichoderma colony on the plate and transfer it to the PDA medium for purification. Purified strains were stored at 4°C.

[0069] Cultivated on PDA medi...

Embodiment 2

[0075] In this example, the antagonism experiment of Trichoderma pseudokoningii on pathogenic bacteria of ginseng was carried out.

[0076]Pathogens of 5 major diseases of ginseng were selected: Alternaria panax, Sclerotinia schinseng, Colletotrichum panacicola, Ilyonectria robusta, and root rot of ginseng (Fusarium oxysporum), the above five ginseng pathogenic strains were provided by the medicinal plant cultivation team of the Institute of Special Products, Chinese Academy of Agricultural Sciences. The preserved Trichoderma Tri112 and pathogen strains were respectively transferred to PDA plates, and after constant temperature cultivation at 25°C in the dark for 7 days, the bacteria cakes were obtained from the edge of the colony with a 5mm hole puncher. The two-point confrontation plate culture method was adopted, and they were placed on the symmetrical sides of the PDA plate respectively, with a distance of 3 cm between the bacteria cakes. Only pathogenic bacteria were inoc...

Embodiment 3

[0080] In this example, an experiment on the growth-promoting effect of Trichoderma pseudokoningii on ginseng cultivated in farmland soil was carried out.

[0081] Pot test method was used to measure the growth-promoting effect of Trichoderma pseudocornifolia Tri112 strain on ginseng. The preparation of the culture medium of the strain: activate the slant of Trichoderma pseudocorniensis Tri112 test tube, take two 5mm bacterial cakes and inoculate them into 100mL potatoes packed in a 250mL triangular bottle In glucose liquid culture medium (PDB), at 170r / min, cultivate 48h at 25 ℃, obtain seed liquid; Trichoderma pseudokoningi Tri112 seed liquid is inoculated in fermentation medium with 10% (volume ratio) and cultivated, at 170r / min Min, cultivated at 25°C for 96 hours to obtain the culture medium; filter the Tri112 culture medium of Trichoderma pseudokoningeni through 2 layers of sterile gauze, count the filtrate through a hemocytometer, and disperse the spore suspension into 6...

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Abstract

The invention discloses trichoderma koningiopsis, a root soaking solution and application, and relates to the technical field of ginseng disease control. The taxonomic name of the strain is trichoderma koningiopsis, and the name of the biological material is Tri112. The trichoderma koningiopsis not only has an antagonistic effect on ginseng pathogenic bacteria, but also has a remarkable growth promoting effect on the growth of ginseng roots, stems and leaves. Farmland soil cultivation ginseng is improved through trichoderma koningiopsis, the soil microbial population structure is protected to a great extent, the biological abundance of the soil microbial population is effectively improved, and soil is more suitable for cultivation of ginseng. The microbial inoculum is applied in the process of planting ginseng in farmland soil, ginseng seedlings are planted, and the relatively high seedling survival rate is guaranteed.

Description

technical field [0001] The invention relates to the technical field of ginseng disease prevention and control, in particular to Trichoderma pseudokoningeni, root soaking liquid and application thereof. Background technique [0002] Ginseng is a valuable perennial medicinal plant with a long growth and development period and relatively strict growth environment conditions. The cultivation mode of ginseng is mainly divided into ginseng planted in forests and ginseng planted in farmland. Since the country has clearly prohibited logging and planting ginseng, the current ginseng planting mode is mainly to grow ginseng in farmland. Due to the problems of low soil bulk density, easy hardening, and low nutrient content in ordinary farmland, ginseng directly planted with ginseng has serious diseases, resulting in low yield and poor quality of ginseng. Moreover, some plots still have the problem of pesticide residues, which are not suitable for the growth of ginseng. [0003] At pre...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCC12N1/14A01N63/38
Inventor 王秋霞马莹莹金桥关一鸣潘晓曦刘宁杨可欣
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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