Preparation method of O-type foot-and-mouth disease virus-like particle antigen, prepared O-type foot-and-mouth disease virus-like particle antigen and application thereof

Abstract

The invention relates to a preparation method of O-type foot-and-mouth disease virus-like particles, which comprises the following steps: recombining a VP0 gene, a VP3 gene and a VP1 gene of an O-type foot-and-mouth disease virus to a pETSUMO vector, forming fusion protein genes with SUMO, amplifying the three fusion protein genes, cloning into the same pET28a expression vector to obtain a recombinant expression vector pET28a-SUMO-VP0-SUMO-VP3-SUMO-VP1, transforming the expression vector into escherichia coli to express fusion protein, and removing SUMO, VP0, VP3 and VP1 proteins from the expressed fusion protein by enzyme digestion under the condition of 10U / mL Ulp1 protease concentration to form the O-type foot-and-mouth disease virus-like particles through self-assembly. The method can be used to efficiently prepares the O-type foot-and-mouth disease virus-like particles, and is suitable for large-scale industrial production.

Description

technical field [0001] The present invention belongs to the fields of molecular biology and virology. The invention specifically relates to a method for preparing an O-type foot-and-mouth disease virus-like particle antigen, the prepared O-type foot-and-mouth disease virus-like particle antigen and applications thereof. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious animal disease that can spread quickly and over long distances. significant economic loss. Animals affected by FMD include cattle, sheep, goats and pigs. The causative agent, foot-and-mouth disease virus (Foot-and-mouth disease virus, FMDV), is a kind of aphthous virus of picornavirus family. The virus is divided into seven serotypes (A, O, C, Asia1, SAT1, SAT2, and SAT3), of which O-type FMDV is the most prevalent. Vaccine immunization is an effective measure to control the disease and protect livestock from harm. Traditional vaccines include inactivated vaccines a...

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Problems solved by technology

However, there are also the following disadvantages: 1. The virus must be produced in highly sealed equipment to prevent contamination of the surrounding environment; during the production process, the virus may not be completely killed or fully attenuated, which will cause the vaccine to contain highly toxic pathogens. The disease substance, which in turn causes the disease of the immunized animals and causes the disease to spread on a large scale

2. The validity period is very short, and often need to be frozen, which increases the difficulty of promoting the use of vaccines in rural areas

3. Inactivated vaccine immunity is indistinguishable from animals infected with FMDV, which restricts the export of animals

4. Production depends on FMDV, so FMDV cannot be completely eliminated

The current use of inactivated vaccines and attenuated vaccines for prevention and control can no longer adapt to the current healthy development of animal husbandry

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